The utilization of methyl bromide (MB) for quarantine purposes has been hampered by its designation as an ozone-depleting substance under the Montreal Protocol. The International Plant Protection Convention's (IPPC) call for alternatives to MB and a reduction in its usage. There is an urgent need to explore and implement substitutes. Despite some substitute agents like EDN being developed for wood, EDN has been limited due to various factors such as occupational risks. This study focuses on evaluating the efficacy of Sulfuryl Fluoride (SF) as a viable alternative fumigant against Reticulitermes speratus, one of major wood destroying pests. Experimental trials conducted at ambient temperature (23°C) revealed promising results, with SF demonstrating LCT50 and LCT99 values of 30.87 mg·h/L and 42.53 mg·h/L, respectively. Under low-temperature conditions (5°C), SF remained effective but with slightly higher LCT50 and LCT99 values of 151.62 mg·h/L and 401.90 mg·h/L, respectively. The penetration test, conducted using R. speratus-infested pine wood cubes, further highlighted SF's efficacy, with LCT50 and LCT99 values of 31.59 mg·h/L and 53.34mg·h/L at 23°C, indicating powerful penetration capabilities. When tested at a loading ratio of 90% (v/v) at 5.0mg/L for 24 hours in a 500L chamber as a middle-scale trial, SF achieved a 100% mortality, showing its potential as a suitable replacement for MB. These findings suggest that SF could open new markets as an MB substitute and enhance safety at quarantine sites when applied to imported and exported timber.