본 연구는 의료기관 간호사의 양육스트레스, 역할갈등이 이직의도에 미치는 영향을 파악하기 위한 서술적 조사연구이다. 본 연구대상은 병원에서 근무하는 간호사로 자녀가 있는 간호사를 142명을 대상으로 하였으며, 수집된 자료의 분석은 SPSS/WIN 23.0 통계프로그램을 이용하여 분석하였다. 대상자의 양육스트레스의 정도는 4점 만점에 평균 2.50점, 역할갈등 정도는 5점 만점에 평균 2.48점, 이직의도 정도 는 5점 만점에 평균 2.71점이었다. 이직의도에 영향을 미치는 변수는 양육스트레스(β=.257 p<.001), 직무 만족(β=.242, p<.001), 역할갈등(β=.161 p<.001)으로 나타났으며 설명력은 총 45.6%이었다. 결론적으로 병원간호사의 이직의도는 양육스트레스, 역할갈등, 직장만족이 영향을 미치는 것으로 나타났다. 따라서 기혼간호사들의 양육스트레스와 역할갈등을 낮추기 위한 방안으로 양육과정에 있는 간호사들의 근무환경 개선 방안이 필요하다.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen Post-thawed in boar. Recently, polymorphism (g. 35756 T>C) of Estrogen Receptor 1 (ESR1) gene reported to be significant association with MOT. This study was conducted to evaluate the ESR1 gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.35756 T>C SNP of ESR1 was significantly associated with frozen semen motility and kinematic characteristics. The g.35756 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.001). The SNP was also significantly associated with ALH (P<0.05). Therefore, we suggest that the g. 35756 T>C polymorphism in the intron 1 region of the porcine ESR1 gene could potentially be applied in frozen semen programs to improve MOT trait, but only after validation in other populations.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen post-thawed in boar. Recently, polymorphism (g.158T>C) of phospholipase C zeta (PLCz) gene reported to be significant association with MOT. This study was conducted to evaluate the PLCz gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.158 T>C SNP of PLCz was significantly associated with frozen semen motility and kinematic characteristics. g.158 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.0001, p= 0.0002, p<0.0001 and p<0.0001, respectively). Therefore, we suggest that the intron region of the porcine PLCz, may be used as a molecular marker for Duroc boar post-thawed semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.

The purpose of this study was to evaluate the sodium-related dietary attitudes, behaviors, and nutritional knowledge in university students according to the practice of dietary guidelines for Koreans established by the Ministry of Health and Welfare, Republic of Korea. Based on the total practice score related to the dietary guidelines (PDG), we classified subjects into a “low practice of the dietary guidelines (LPDG)” group (n=94, male=43, female=51) (total score of PDG ≤13) and a “high practice of the dietary guidelines (HPDG)” group (n=56, male=32, female=24) (total score of PDG >13). Subjects were asked about general characteristics, lifestyle, salt-related dietary attitudes, behaviors, and nutritional knowledge using a questionnaire. The LPDG group had more subjects who were breakfast skippers (p<0.001) compared to the HPDG group. The LPDG group (31.26) had a significantly lower score in terms of salt-related dietary attitudes compared to the HPDG group (33.77) (p=0.0042). The score for salt-related dietary attitudes was significantly higher in the HPDG (32.52) group than in the LPDG (29.91) group (p=0.0041). There was no significant difference in the total score for salt-related nutrition knowledge between the groups. The correlation analysis indicated that the dietary guidelines practice score had a positive correlation with the salt-related dietary attitude score (r=0.3593, p<0.0001) and the dietary attitude score (r=0.3443, p<0.0001) after adjustments for sex. These results show that the degree of adherence to the dietary guidelines for adults may be related to sodium-related dietary attitudes, behaviors, and nutrition knowledge.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen post-thawed in boar. Recently, polymorphism (g.358A>T) of cluster-of-differentiation antigen 9 (CD9) gene reported to be significant association with MOT. Also, CD9 gene was expressed in the male germ line stem cells is crucial for sperm-egg fusion, and was therefore selected as candidate gene for boar semen. This study was conducted to evaluate the pig SNP (g.358A>T) of CD9 gene as a positional controlling for semen parameters of post-thawed boar semen. To results, the g.358A>T SNP of the CD9 gene was significantly associated with the traits such as MOT, curve linear velocity, straight line velocity, average path velocity and amplitude of lateral head displacement. Particularly, the g.358A>T SNP significantly has the highest association with MOT and animals with AA genotype (p<0.001). Therefore, we suggest that the g.358A>T in the intron 6 region of the porcine CD9 may be used as a molecular marker for Duroc boar Post-thawed semen quality, although its functional effect was not defined yet.

Cynegetis impunctata (Linnaeus, 1767), a phytophagous lady beetle, was recorded in South Korea for the first time recently. Previously, only the adult form of this species had been described. In this study, we provide a redescription of the adult and the first descriptions of the larva and pupa. Diagnostic characters for this species are given, and intraspecific color variation in Cynegetis impunctata adults is discussed.

The boar sperm has more lipid droplets and specialty of seminal plasma compared with other species, causing difficulties of freezing sperm and decreases for the utilization of frozen semen into the artificial insemination. However, several studies reported significant results for the recovery of sperm motility and reproductive by addition of cryoprotectants and seminal plasma after thawing. This study was designed to investigate the effects of supplementation of trehalose or glycerol in the LEY (lactose and egg yolk in BTS) solution for the conventional freezing and vitrification process. Two boars aged 16 months were used to collect semen for 2 times in a week. The samples were allotted to 3 freezing solutions (LEY + glycerol 10.5% + OEP 1.5%, LEY + trehalose 1M + OEP 1.5%, and sucrose 1.5M + trehalose 1 M + OEP 1.5%) after centrifugation at 800 g for 10 minutes. Semen was equilibrated in freezing solutions for 10 minutes and injected into plastic straws with 2∼3 air bubbles to minimize freezing damages. Vitrification was performed to locate sperm in 5 cm above LN2 for 5 minutes, and the conventional freezing was conducted with an automatic freezer. Motility and survival rates were measured by CASA (Computer assisted sperm an alyzing system) and FITC (Fluorescein isothiocyanate), respectively after thawing semen at 50℃ for 12 seconds. The results were analyzed by ANOVA with STATVIEW statistical program. The vitrificatioin solution (LEY + 10.5% glycerol + 1.5% OEP) presented higher motility (20.9%) than other solutions while the solution (LEY + 1M trehalose + 1.5% OEP) showed the lowest (motility : 5.2%). However, survival rates of vitrified sperms detected by FITC showed 1~4% live sperms in almost of dead sperms at all vitrification solutions’ groups, but survival rate of freezing solution of LEY + 1M trehalose + 1.5% OEP LEY and LEY + 10.5% glycerol + 1.5% OEP were showed 49%, and 79%, respectively. There were differences (P<0.05) survival rate of conventional freezing in LEY + 10.5% glycerol + 1.5% OEP and LEY + 1M trehalose + 1.5% OEP and the remaining showed no differences. The results suggested that vitrified boar semen was not enough to be utilized for the artificial insemination, but it showed possibility to utilize for ICSI and conventional freezing with glycerol would be useful method for artificial insemination in pig while we choose the outstanding semen against tolerance to freezing damages.

본 연구는 단일 입력 전이함수모형(Single-input transfer function model)을 적용하여 여수연안 2010년의 월평균 표면수온의 예측을 시도하였다. 전이함수모형을 수립하기 위한 입력시계열과 출력시계열은 각각 여수지방의 10년(2000-2009년)간의 월평균 기온자료와 표면수온자료를 이용하였다. 전이함수모형을 수립하기 위하여 입·출력 시계열을 사전백색화하고, 입·출력 시계열간의 각 시차에 대한 교차상관함수를 결정하였다. 교차상관함수는 음의 모든 시차에서 유의한 값을 갖지 않아 기온과 표면수온사이는 일방적 인과관계를 보였다. 또한 교차상관함수의 시차 0과 1에서 유의한 값을 보였다. 이러한 교차상관함수의 특징에 따라 입·출력시계열간 전이함수의 시차와 분모 및 분자의 차수(b, r, s)는 (0, 1, 0)으로 식별되었다. 구축된 전이함수모형에 따르면 기온과 표면수온 사이의 시차는 존재하지 않았다. 여기서 현재의 표면수온은 1개월 전의 표면수온과 선형관계가 있음을 보였으며, 잡음모형은 ARIMA(1,0,1)(2,0,0)12로 식별되었다. 전이함수모형에 의한 월평균 표면수온의 예측치는 실측치에 비하여 전반적으로 0.3-1.3℃ 높은 경향을 보였으며, 6.4 %의 평균절대백분율 오차를 포함하였다. 이러한 결과는 8.3 %의 평균절대백분율오차를 보인 ARIMA 모형에 비하여 향상된 예측성능을 보이는 것이며, 표면 수온의 시계열적 예측을 시도할 경우, ARIMA 모형보다 전이함수모형의 적용을 통하여 그 예측성능의 개선 가능성을 기대할 수 있음을 시사하고 있다.

본 연구는 날치 난소막에서 추출한 날치 난소막 추출물(Flying fish Roe Shell Extract, 이하 FRSE라 명함)의 물리화학적인 특성을 조사하기 위하여 수행되었다. 해양성 원료인 날치 난소막에서 FRSE를 제조하여 물리화학적인 특성을 분석하였다. 영양성분 조성을 분석한 결과 FRSE는 81.00%의 단백질과 9.12%의 회분, 그리고 4.48%의 수분으로 구성되어있는 것으로 나타났다. 아미노산 조성분석 결과 FRSE는 콜라겐 펩타이드의 특징인 OH-proline과 glycine이 검출되었으며, 포도당과 지방 대사에 관여하는 glutamic acid 와 aspartic acid 함량이 높게 검출되었다. 또한 열량분석 결과에서는 100g의 FRSE가 347 kcal의 열량을 지니고 있는 것으로 나타났다. FRSE의 분자량 분석 결과에서는 약 1,300 Da정도의 평균분자량 분포를 나타낸다는 것을 알 수 있었다.

들기름 및 미강유를 베이스로 개발한 혼합 식용유는 오메가-3 지방산인 리놀렌산 함량이 약 20% 정도로 매우 높다. 이는 기존의 일반 식용유 중 오메가-3 지방 함량이 매우 높은 콩기름과 카놀라유의 리놀렌산 함량이 각각 6% 및 10% 내외인 점을 감안하면, 이들 식용유에 비하여 새로 들기름과 미강유를 혼합한 식용유는 2~3배 높은 비율로 오메가-3 지방산을 제공할 수 있어서, 오메가-3 지방산 섭취가 부족한 현대인의 식단에서 중요한 오메가-3 지방산 급원으로서의 역할을 할 수 있을 것이다. 뿐만 아니라, 토코페롤 함량도 약 46.77 ㎎/ 100 g oil 정도인데, 가장 주목할 일은 토코페롤 동족류 중 비타민 E의 활성이 가장 강한 알파-토코페롤(31.71 ㎎/100 g oil)이 가장 많이 존재하여, 총 토코페롤의 64%를 차지하고 있어서 비타민 E로서의 기능이 높다. 혈중 콜레스테롤 함량을 낮추는 기능성이 알려진 파이토스테롤 함량이 들기름과 미강유를 혼합한 식용유에 약 7,200 ppm 함유되어 있어, 일반적인 식용유의 3,000 ppm의 약 2.3배에 달한다. 본 연구에서 개발한 들기름과 미강유를 혼합한 식용유는 일반적인 식용유인 콩기름과 거의 유사한 산화안정성을 갖는 것으로 확인되어, 일반 들기름에 비하여 현저히 높은 산화안정성이 확보되었다.

This study was conducted to investigate the changes of hormone levels of serum progesterone (P4) and estradiol-17 β (E2) in sows of Landrace (L), Yorkshire (Y) and F1 (L × Y) (respectively n=3) with excellent ability, and to provide a baseline data for improving reproductive performance. In this experiment, the sows at the age of 12 months or more were used. The sows were fed by two way methods, one is conventional methods and the other is 3 days-flushing feed before estrus. Each pig’s blood was collected in 3, 6, 9, 12, 15 and 18 days after the estrus for the analyses of P4 and E2. Serum was separated by centrifugation for 15 min. with 3,000 rpm. Progesterone and estradiol-17β were measured by immunochemical assay (ELIZA test). In conventional feeding, serum progesterone levels were significantly (p<0.01) higher in F1 than in L and Y. No significant differences in P4 concentrations were seen between the L and Y of sows. Serum E2 levels were similar the serum progesterone levels. In the case of flushing feed, the tendency of hormonal changes were similar to conventional methods. But almost of hormonal levels were a little higher than that of conventional methods. P4 level of L and Y in flushing feed were significantly different (p<0.01). Serum E2 level of Y in flushing feed was significantly different among the breeds (p<0.01). These results were similar to the tendency of hormonal changes in general sows and moreover, flushing feed is known to develop the swine production, these results proved the fact of the methods. And these results suggested that more studies about hormonal changes in sows according to seasonal and nutritional factors should be needed.

In this research we extracted water-soluble collagen peptide from flatfish skin and compared it with commercially available collagen peptide extracted from Tilapia scale currently placed on the market in the aspect of physiochemical property. The physical property and nutritional components of FSCP appeared almost similarly to those of TSCP, and also in calorie, FSCP marking 3.82 Kcal showed no differences from TSCP marking 3.84 Kcal. As for forming amino acids, in aspartic acid, serine, histidine, tyrosine, methionine, FSCP had higher content than TSCP, but in OH-proline, proline and alanine FSCP had lower content than TSCP. Especially the content of essential amino acids of FSCP marked 22.74% with a higher content compared with 13.64% of TSCP. In the distribution of molecular weight FSCP with 1,000 Da showed omparatively low compared with TSCP, and in emulsion property and stability FSCP and TSCP showed similar excellent trend.

Benthic macroinvertebrates were seasonally sampled from upstream and downstream of the Gunwi Dam construction site from 2006 to 2012. Totally 148 and 165 species were collected at the upstream and downstream sampling sites, respectively. At the upstream area after dam construction, there was no significant change of the numbers of non-insect taxa, but at the downstream area, the non-insect taxon numbers were increased gradually. The individual ratio of Ephemeroptera and Diptera had higher value than the other taxa in both of up and downstream. The trichopteran individual ratios were increased in upstream sites, but decreased in downstream sites from 2010. Also, non-insects and coleopteran individual ratios were decreased in upstream, but increased in downstream after completion of dam construction. The annual range of fluctuation in community indices narrowed after construction. According to a functional feeding group analysis, the individual ratios of FC (Filtering Collector) were increased, and GC (Gathering Collector) and SC (Scraper) were decreased in upstream sites annually. In contrast, the annual individual ratios of FC were decreased, and GC and SC were increased in the downstream sites.