This study assessed the efficacy of aqueous chlorine dioxide (CIO2) and commercial chlorine sanitizer in terms of its ability to eliminate Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli 0157:H7 on radish sprouts (Raphanus sativus L.). Radish sprouts were inoculated with a cocktail containing one each of three strains of three different foodbome pathogens, then treated with distilled water (control) or chemical sanitizers (100 ppm commercial chlorine, and 50, 100, 200 ppm CIO2) for 1, 5, and 10 min at room temperature (22士2℃). Populations of S.Typhimurium, E. coli 0157:H7 and L. monocytogenes were counted at 4.64, 6.05, and 4.29 log CFU/g, respectively, after inoculation. Treatment with water did not significantly reduce the levels of any of the three foodbome pathogens. The levels of all three pathogens were reduced by treatment with chemical sanitizers; however, the observed 1evels of reduction of E. coli 0157:H7 and L. monocytogenes were not significant as compared with the controls. The levels of the three pathogens were reduced most profoundly when treated for 10 min with 200 ppm of CIO2, and the reduction levels of S. Typhimurium, E. coli 0157:H7, and L. monocytogenes were 1.17, l.63, and 0.9610g CFU/g, respectively. When chemically injured cells were investigated using SPRAB for E. coli 0157:H7 and by selective overlay methods for S. Typhimurium and L. monocytogenes, respectively, it was noted that commercial chlorine sanitizer generated more numbers of Í넌red pathogens than did CIO2. These data indicate that CIO2 treatment may prove useful in reducing the numbers of pathogenic bacteria in radish sprouts.