The purpose of this study was to investigate the effects of sterilization, storage period and washing yuzu, according to the washing method, during the storage period. The results showed that the fungus size increased as the storage period increased, and no mold occurred in the yuzu washed with ozone water until 20 days. After 30 days of storage, a mold of 124.1±13.9 mm2 was observed. The no-treatment sample had a fungus of 814.5±72.8 mm2 in size on day 0 and the fungus the largest fungus was 6,362±636.7 mm2 on day 30. In the case of water treatment, the fungus was 286.4±31.5~4,836.4±484.6 mm2 in size. The results of the study confirmed that washing yuzu with ozone water has a sterilizing effect.

본 연구는 국내 원자력방사선 관련 전시 및 교육 발전 방향을 모색하기 위하여 국내 원자력 홍보관의 전시물 및 전시패널을 내용에 따라 크게 4가지 범주로 분류하고, 과학커뮤니케이션의 6가지 요소로 분석하여 비율을 파악하였 다. 연구 대상은 국내 원자력 홍보관 총 4곳을 목적 표집하였으며, 일본의 원자력 과학관 1곳을 선정하였다. 연구 결과, 국내 모든 기관에서는 4가지 범주 중에서 개념에 해당하는 내용이 높은 비율로 나타났다. 그리고 전시물에 포함되어 있는 과학커뮤니케이션 요소는 개념(CON)과 흥미(INT)가 주를 이루었으며, 반면에 과학의 본성(NOS), 인식(AW), 즐거 움(ENJ), 의견(OP)은 제한적으로 나타났다. 본 연구결과를 통하여 다음과 같은 결론을 내릴 수 있다. 원자력 홍보관은 관람객에게 원자력방사능에 대한 다양한 측면의 정보를 제공하여 관람객 스스로 올바른 판단을 하고 과학적 태도를 향상시키며 과학커뮤니케이션 요소의 이해를 높일 수 있도록 과학적 소양인 양성을 위한 전문 비형식 교육기관으로써의 역할을 수행할 수 있어야 한다. 그리고 전시물에 반영하기 어려운 과학커뮤니케이션을 충족시킬 수 있도록 전문 도슨트 또는 해설사 양성을 위한 전문 프로그램 개발과 적용에 대한 지속적인 연구가 필요하다.

Soybean [Glycine max (L.) Merr.] protein is excellent nutritional factors and is widely used for human and animal feed in the world. Glycinin(11S globulin) and ß-conglycinin(7S globulin) account for storage protein from 70% to 80% in soybean seed. ß-conglycinin is composed of α’, α and ß subunits, encoded by the genes Cgy1, Cgy2, and Cgy3, respectively. β-conglycinin protein exhibits poor nutritional and food processing properties. Lipoxygenase is responsible for the beany flavor and is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The objective of this research was to select F2 seeds with lipoxygenase-free and low content of ß-conglycinin for breeding of high quality soybean cultivar. A total of 582 F2 seeds were obtained from the cross of 10F1(lipoxygenase-free, normal content of 7S protein) and T311(lipoxygenase-present, low content of 7S protein). Lipoxygenase and 7S proteins in mature seeds were detected by SDS-PAGE. The segregation ratio of 3 : 1 for Shr locus (418 Shr_ : 164 shrshr) were observed. This result shows that the Shr locus is controlled by a single gene. Among 164 shriveled seeds, 6 F2 seeds with lipoxygenase-free, α’ subunit-free, and low content of α and ß subunit were selected. Among 418 normal seeds, 6 F2 seeds with lipoxygenase-free, α’ subunit-free, and low content of α and ß subunit were selected. These results will be used in breeding program for improving high quality soybean cultivar.

Soybean [Glycine max (L.) Merr.] proteins are widely used for human and animal feed in the world. Glycinin (11S globulin) and β-conglycinin (7S globulin) account for storage protein from 70% to 80% in soybean seed. 7S globulin protein exhibits poorer nutritional and food processing properties. β-conglycinin is composed of α’, α, and β-subunits. α′-subunit of 7S globulin are main antinutritional factors in soybean seed. The absence of α′-subunit were controlled by single recessive alleles, cgy1. Leaf chlorophyll-deficient mutants controlled by y9 locus have been observed frequently and are useful in genetic studies in soybean. So far, 19 single recessive gene yellow leaf mutants and one duplicate recessive gene mutant have been described. The y9 type found in T135 is yellow at emergence, becoming greenish-yellow by maturity. The objective of this research was to confirm the linkage or independent assortment between cgy1 gene and y9 gene. The F1 seeds from C-142 (α′-subunit of 7S globulin present, leaf chlorophyll -deficient: Cgy1Cgy1y9y9 genotype) x PI line (α′-subunit of 7S globulin absent, normal leaf type: cgy1cgy1Y9Y9 genotype) were obtained. F1 seeds obtained were planted in a greenhouse and F1 hybridity was checked on morphological traits. All F2 seeds were planted at field in May 2012. Leaf chlorophyll-deficient trait of F2 individual plants will be recorded at growth stage from field. Presence and absence of α′-subunit of 7S globulin protein will be checked by SDS-PAGE based on each F₂single plant. Chi-square analysis was used to test the goodness-of-fit of observed ratios with expected ratios for independent assortment or linkage.

Leaf chlorophyll-deficient mutants controlled by y9 locus have been observed frequently and are useful in genetic studies in soybean. So far, 19 single recessive gene yellow leaf mutants and one duplicate recessive gene mutant have been described. The y9 type found in T135 is yellow at emergence, becoming greenish-yellow by maturity. Soybean Kunitz trypsin inhibitor protein has been proposed as one of the major antinutritional factor. The absence of Kunitz trypsin inhibitor protein in mature seed is inherited as a recessive allele designated ti. The objective of this research was to confirm independent inheritance between ti gene and y9 gene. The F1 seeds from Gaechuck#1 (Kunitz trypsin inhibitor protein absent, normal leaf type) x C-142 (Kunitz trypsin inhibitor protein present, leaf chlorophyll -deficient) were obtained. F1 seeds obtained were planted in a greenhouse and F1 hybridity was checked on morphological traits. All F2 seeds were planted at field in May 2012. Leaf chlorophyll-deficient trait of F2 individual plants will be recorded at growth stage from field. Presence and absence of Kunitz trypsin inhibitor protein will be checked by SDS-PAGE based on each F₂single plant. Chi-square analysis was used to test the goodness-of-fit of observed ratios with expected ratios for independent assortment or linkage.

The hydrological frequency of the flood in July 2000 at Seosi stream basin in Gurye and the bed scour of the stream channel were estimated to investigate the bed scour related with Jeongjang bridge collapse. The storm over the basin in July 2000, 303mm/day was 103year frequency rainfall and the equivalent flood was 2580cms. As the results of 100year and 30year flood application, flood level 30.78～31.38m and mean velocity 3.79～4.03m/s were appeared. And the purification project of Seosi stream increased the velocity of the section near to Jeongjang bridge by the improvement of conveyance at the downstream. The local scour at pier was the major factor of bed scour at Jeongjang bridge site and the total scour at pier No.6 was increased from 2.32m to 2.45m by the purification project.