Recently, Hypsizigus marmoreus is noticed as new mushroom item in Korea. However, this mushroom has the long incubation period about 80 days including pre-incubation period. This study was attempted to establish the optimal post-incubation temperature and period of Hypsizigus marmoreus for bottle cultivation. The most effective post-incubation temperature and period were 25℃ and 20days, respectively. The higher yield was obtained under 25℃ for 20 day after 30 days of pre-incubation. Therefore, total cultivation period was shortened from 106 days (control) to 78days. It is useful information to increase rotation time of cultivation for effective management in H. marmoreus.