In order to breed the useful mutants in black raspberry, cultivated in Gochanggun, leaf-petiol explants from shootproliferating culture was regenerated after irradiated with 30, 50 and 70 Gy gamma-ray and seeds were irradiated with 100, 130, 150, 170 and 200 Gy gamma-ray. The LD50 dose of leaf-petiol explants and seeds in black raspberry was identified as 30 and 150 Gy after two months and one month, respectively. The mutants such as leaves with increasing width, chlorophyll content and increasing anthocyanin were selected in regenerated explants and seeds irradiated. The increased rate of leaves width and chlorophyll were 33.3% and 23.1% compared to the control. Also, antocyanin concentration in leaf of mutnats from irradiated seeds was increased till 17.3%. For the breeding of black raspberry, valuable mutants obtained will be expected for improving photosynthesis efficiency and functional components, and will be useful for developing new cultivars.