In this paper, we propose adaptive convergence security policies and management technologies to improve security assurance in the home networking environment. Many security issues may arise in the home networking environment. Examples of such security iss

In a pilot-scale dyeing wastewater treatment using two-type fluidizing media, each thickness of biofilm was 15 and 30 μm, respectively. The numbers of protozoa inhabited in small-size (PEMT A) and big-size (PEMT B) media were 7.5 x 104 and 1.25 x 105 cells/ml, respectively, and dominant species were Entosiphon sulcatus var sulcatus in PEMT A and Chlamydomonas reinhardtii in PEMT B, respectively. Flask experiments using the two media revealed that the percentages of color removal were 25.8% in PEMT A and 27.1% in PEMT B after 72-h cultivation, indicating the necessity of bioaugmentation. Experiments for bioaugmentation effect on color removal were carried out in the pilot-scale treatment for 75 d by three-step operation under the control of wastewater loading rate and microbial input rate. Dye degradation occurred mainly in the second reaction tank, and the attachment of augmented dye-degrading microorganisms to media took at least 35 d. Final value of chromaticity in effluent was 227, meeting the required standard. Therefore bioaugmentation onto media was good for color treatment. In summary, thickness of biofilm formed on the media depended upon the size of media, resulting in different ecosystem inside the media. Hence, this affected microbial community and color treatment further. Accordingly, the reduction of operation cost is expected by efficient color-treatment process using bioaugmented media.

This study was performed to evaluate the asbestos exposure levels and to calculate excess lifetime cancer risk (ELCR) for the risk assessment of the asbestos fibers released from asbestos-cement slate roofing (ASR) building. Total number of ASR buildings was into 21,267 in Busan, and 82.03 percent of the buildings was residential houses, and 43.61 percent of the buildings was constructed in 1970s. For this study, ten buildings were selected randomly among the ASR buildings. The range of airborne asbestos concentration in the selected ten ASR buildings was from 0.0016 to 0.0067 f/mL, and the concentration around no-admitted ASR buildings was higher than that around admitted buildings. The ELCR based on US EPA IRIS (integrated risk information system) model is within 3.5E-05 ~ 1.5E-04 levels, and the ELCR of no-admitted ASR buildings was higher than 1.0E-04 (one person per million) level that is considered a more aggressive approach to mitigate risk. These results indicate that the cancer risk from ASR buildings is higher than other buildings, and systematic public management is required for control of no-admitted ASR buildings within near future.

The concentration of organic compounds was analyzed at each step of BAC process though BDOCtotal/rapid/slow. Further, bacteria communities and biomass concentrations measured FISH and ATP methods were analyzed. The bed volume (BV) of steady state is different from that of based on assessment of organic compounds removal. Bed volumes in DOC, BDOCrapid and BDOCtotal/slow removal at steady state were around 27,500 (185.8 day), 15,000 (101.4 day) and 32,000 (216.2 day), respectively. A biomass didn't change after the bed volume reached 22,500 (152.0 day) according to analyzing ATP concentration of bacteria. The concentration of ATP was 2.14 μg/g in BV 22,500 (152.0 day). The total bacterial number was 4.01±0.4×107 cells/g at the bed volume 1,150 (7.8 day) (the initial operation) and the number of bacteria was 9.27±0.2×109 at the bed volume 58,560 395.7 day) that increased more than 200 times. Bacterial uptrend was reduced and bacterial communities were stabilized since BV 18,720 (126.5 day). When BV were 1,150 (7.8 day), 8,916 (60.2 day), 18,720 (126.5 day), 31,005 (209.5 day), 49,632 (335.3 day), 58,560 (395.7 day), a proportion of total bacteria for the Eubacteria were 60.1%, 66.0%, 78.4%, 82.0%, 81.3% respectively. γ-Proteobacteria group was the most population throughout the entire range. The correlation coefficient (r2) between Eubacteria biomass and ATP concentration was 0.9448.

To find out the growth conditions for the maximum activity of nitrogenase which catalyzes nitrogen fixation in Rhodobacter sphaeroides, the promoter activities of nifA and nifH were analyzed and the results indicated that expression of both nifA and nifH was increased in response to deprivation of both O2 concentration and nitrogen source. The nifA mutant was constructed by deleting the gene to investigate the effect of NifA, the transcriptional regulator, on the nifH and nifA expression in R. sphaeroides. Analysis of expression of nif genes using the nifA::lacZ and nifH::lacZ fusions in the nifA mutant revealed that NifA acts as a positive activator for nifH and an autoregulator in its own expression. The promoter activities of nifA and nifH in the prrA mutant grown under anaerobic and NH4 +-free conditions were derepressed, comparing with those of the wild-type grown under the same conditions, indicating that the prrA product acts as a positive regulator in expression of nifA and nifH.

This study was performed to evaluate the asbestos exposure levels and to calculate excess lifetime cacer risks(ELCRs) in asbestos-containing buildings for maintenance and management. The range of airborne asbestos concentration of 33 buildings was 0.0018 ~ 0.0126 f/cc and one site exceeded indoor air-quality recommended limit 0.01 f/cc. And ELCRs based on US EPA IRIS(Integrated risk information system) model are 1.5E-06 ~ 3.9E-05 levels, and there was no site showed 1.0E-04 (one person per million) level or more, and 11 sites showed 1.0E-05 (one person per 100,000 people) level or more. To prevent the release of asbestos fibers, it needs operation and maintenance of asbestos-containing building materials, and there are some methods such as removal, repairment, enclosure and encapsulation. In conclusion, a risk-based air action level for asbestos in air is an appropriate metric for asbestos-containing building management.

A bacterial strain SY-97 that showed algicidal activity against Cochlodinium polykrikoides was isolated from coastal water of Uljin (eastern coast of Korea) in August, 2005. The isolated strain was identified as Brachybacterium sp. by morphological and biological tests, and analysis of 16S rDNA sequence. The optimal culture conditions for the growth of strain SY-97 were 30℃, initial pH 7.0, and salinity 2.0%. From the result of cell culture insert experiment, Brachybacterium sp. SY-97 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides (1.2 × 104 cells/㎖) cultures, 100% of C. polykrikoides cells was destroyed within 15 hours. The released algicides were heat-tolerant to 100℃ and stable in pH 6.0～10.0. These results suggest that Brachybacterium sp. SY-97 is potentially useful for controlling outbreaks of C. polykrikoides.

The bacterial community structure in biological activated carbon (BAC) process in drinking water treatment plant was investigated by Fluorescent in situ Hybridization (FISH) with rRNA-targeted oligonucleotide probe. Samples were collected at different three points in BAC process every month for one year. They were hybridized with a probe specific for the alpha, beta, gamma subclass of the class Proteobacteria, Cytophaga- Flavobacteria group and Gram-positive high G+C content (HGC) group. Total numbers of bacteria in BAC process counted by 4',6-diamidino-2-phenylindole (DAPI) staining were 5.4×1010 (top), 4.0×1010 (middle) and 2.8×1010 cells/ml (bottom). The number of the culturable bacteria was from 1.0×107 to 3.6×107 cells/ml and the culturability was about 0.05%. The faction of bacteria detectable by FISH with the probe EUB338 was about 83% of DAPI counts. Gamma and alpha subclass of the class Proteobacteria were predominant in BAC process and their ratios were over 20% respectively. In top and middle, alpha, beta and gamma subclass of the class Proteobacteria competed with each other and their percentages was changed according to the season. In bottom, gamma subclass of the class Proteobacteria was predominant all through the year. It could be successfully observed the seasonal distribution of bacterial community in biological activated carbon process using FISH.

Algicidal bacterium was isolated from sea water during the declining period of Cochlodinium polykrikoides blooms and this bacterium had a significant algicidal activity against C. polykrikoides. In this study, algicidal bacterium was identified on the basis of biochemical and chemotaxonomic characteristics, and analysis of 16S rDNA sequences. The algicidal bacterium showed 98.6% homology with Micrococcus luteus ATCC 381T. Therefore, this bacterium was designated Micrococcus luteus SY-13. The optimal culture conditions of the algicidal bacterium was 25℃, initial pH 8.0, and 3.0% NaCl concentration. M. luteus SY-13 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides (1.0 × 104 cells/㎖) cultures, over 98% of C. polykrikoides cells were destroyed within 6 hours. The culture filtrate of M. luteus SY-13 exhibited similar algicidal activity after heat-treatment at 121℃ for 15 min. While algicidal activity remained in filtrates with pH adjusted to 8.0, loss of algicidal activity occurred when the pHs of filtrates were adjusted to over 9.0 or heat-treated at 121∼180℃ for 1 hour. M. luteus SY-13 showed significant algicidal activities against C. polykrikoides (98.9%) and a wide algicidal range against various harmful algal bloom (HAB) species. However, there was no algicidal effect on diatom and marine livefood organisms except Isocrysis galbana. These results suggest that M. luteus SY-13 could be a candidate for use in the control of HABs.

From sludge of S municipal wastewater treatment plant in Busan, Korea, we isolated the denitrifier DN-9 which showed the ability of denitrification under aerobic condition by the color change and gas formation in liquid culture with Giltay medium. The isolated strain was identified as Pseudomonas sp. DN-9 on the basis of the morphological, physiological, biochemical and nucleotide sequence analysis of 16S rRNA. The isolated strain, Pseudomonas sp. DN-9, has cytochrome cd1 nirS of nitrite reductase. By the co-existance of additional ammonium and nitrate ion, the strain was not affected largely on growth in SL series broth. It seemed the result of denitrification. Although Pseudomonas sp. DN-9 has a good nitrate reduction activity under aerobic condition, the activity is less than Pseudomonas stutzeri in same cultivation condition. However, Escherichia coli had little the activity of aerobic denitrification and Pseudomonas putida showed lower activity of aerobic denitrification than Pseudomonas sp. DN-9 and Pseudomonas stutzeri in this study.

The activated sludge from the aeration basin of the Su-yeong municipal wastewater treatment plant which has operated by a standard activated sludge process in Busan, Korea was investigated during April 2004 and January 2005 with several bio-indicators. The number of bacteria and fungi per gram of dry weight of MLSS were estimated to be 3.1×106~1.5×108 and 1.1×103~1.1×105 colony forming units, respectively, by the plate agar method. By cultivation-independent methods, such as 4’,6-diamidino-2-phenylindole stain and fluorescence in situ hybridization, the ratio of eubacteria to the entire biomass was evaluated by more than 80% (v/v). The ratio of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria to the total eubacteria was determined to be 7.0~9.8% and 3.3~6.2% without heavy variation in spite of a period of relatively low temperature in the basin. It would be expected that the nitrification would occur or at least co-exist throughout the year in the sludge of many municipal WWTP with influents that contain the sufficient nitrogen sources although the WWTP does not have any specialized processes for the removal of nitrogen.