The objective of this study was to evaluate several types of uterine bacteria in Hanwoo. uterine bacteria from randomly selected 5 uterus was collected by flushing methods into a sterilized 1.5 ml centrifuge tube and was inoculated onto MacConkey agar and blood agar, respectively. After being incubated for 5% CO2, aerobic or anaerobic condition at 37℃ during 48h, bacterial colonies were selected and re-inoculated onto blood agar plates. Re-cultured colonies were identified by Gram staining and finally identified using Vitek system. The identified bacteria were Staphylococcus lentus, Staphylococcus sciuri, Staphylococcus vitulinus, Staphylococcus warneri of Gram (+) and Rhizobium radiobacter, Sphingomonas paucimobilis of Gram () bacteria. Although, pathogenicity of identified bacteria was unclear, the bacteria can have an effect on the uterine microenvironment. Therefore, repetitive research will be required to determine the effects of bacteria in cattle exposed to a various environment.

Weissella viridescens와 Leuconostoc carnosum은 육가공 제품 변패 미생물 중에서 중요한 미생물 로 인식되어 왔다. 용아초(Agrimonia pilosa)는 아시아에 널리 분포해 있으며, agrimonolide, agrimophol, agrimoniin, agrimol, agrimonic acid A, agrimonic acid B와 같은 다양한 플라보노이드를 함유하고 있다. 본 연구에서는 용아초의 추출방법에 따른 W. viridescens와 Leu. carnosum에 대한 생육저해효과를 탐색하였다. pH 11로 조정된 물 추출물과 50%(v/v) 에탄올 추출물은 W. viridescens 와 Leu. carnosum에 대하여 각각 43%와 24%에 해당하는 생육저해효과를 나타내었다. 그러나 사용된 추출용매와 상관 없이, 추가적인 초음파 추출은 용아초 추출물의 항균활성 증진에 큰 영향을 미치지 않았다.