As awareness about the danger of radon in indoor air has increased, various studies have been conducted to reduce the source of radon. This study was performed to investigate the effect of radon mitigation technology in a railway tunnel. Radon barrier paint and radon shield membrane developed to reduce the concentration of radon in soil and construction material were applied in the tunnel. The tunnel was divided into three sections, A, B, and C, and radon barrier paint, a buffer section, and radon shield membrane were applied, respectively. After securing a sealing screen to the floor and division of each section, radon concentrations were measured and compared before and after each product was applied, and statistical significance was confirmed through the Wilcoxon signed rank test. Measurement was performed with the In-Situ Method and Closed Chamber Method. Radon concentration measured by the in-situ method changed in A section to 124.1 Bq/m2/day from 614.1 Bq/m2/day (79.8%, z=-2.521, p<0.05), in B section to 416.2 Bq/m2/day from 467.1 Bq/m2/day (10.9%, z=-0.980, p=0.327), and in C section to 47.3 Bq/m2/day from 645.6 Bq/m2/day (92.7%, z=-2.521, p<0.05). Radon concentration measured by the closed chamber method recorded a decrease in A section to 88.8 Bq/m3 from 364.2 Bq/m3 (75.6%, z=-2.201, p<0.05), in B section to 471.8 Bq/m3 from 583.3 Bq/m3 (19.1%, z=-0.700, p=0.484), and in C section to 115.9 Bq/m3 from 718.8 Bq/m3 (83.9%, z=-2.521, p<0.05). In addition to soil, it is very important to mitigate radon from building materials with a high contribution rate of radon in order to manage radon by source. Due to the spatial characteristics of railway tunnels, soil and wall concrete structures are exposed as they are, so it is considered that radon mitigation actions are required utilizing verified methods with high mitigation efficiency.

Vitrification has been suggested to be an effective method for the cryopreservation of human ES cells. However, the efficiency of vitrification with different vehicles remains a matter of ongoing controversy. The objective of this study was to assess the efficiency of cryopreservation in human ES cells by vitrification using different vehicles. A human ES cell line and a variety of vehicles, including microdroplet (MD), openpulled straw (OPS) and electron microscopic grid (EMgrid), were employed in an attempt to assess vitrification efficiency. In order to evaluate the survivability and the undifferentiated state of the postvitrified human ES cells, we conducted alkaline phosphatase staining and characterization via both RTPCR and immunofluorescence assays. The survival rates of the postvitrified human ES cells using MD, OPS and EMgrid were determined to be 61.5%, 66.6% and 53.8%, respectively. There also exist significant differences between slowfreezing and vitrification (p<0.01). However, no significant differences were detected between the vehicle types. Finally, the pluripotency of human ES cells after thawing was verified by teratoma formation. Cryopreservation using vitrification is more effective than slowfreezing, and the efficiency of vehicles proved effective with regard to the preservation of human ES cells.