To elucidate optimum environmental condition for artificial cultivation of Grifola frondosa, light condition and CO2 concentration have been examined. First, the effects of light quality(blue, green, white) and light intensity(200, 500, 800, 1200 lux) on primordia formation, fruit body yield and morphological properties of Grifola frondosa in bag culture method were tested. Among different color fluorescents, white light(400∼620 nm) had a high mushroom yield(242 g) and a shorter cultivation period(52 days) In particular, morphological properties of G. frondosa using blue light(400∼560 nm) was obtained a wide and deep colored pileus. We also investigated that the appropriate light intensity for production of G. frondosa. The experimental results indicated that 500 lux light was the most effective on mushroom production, whereas, primordia formation was effective at 200 lux. In summary, we propose that the optimal light condition for the bag culture of G. frondosa is white source of fluorescent light and 500lux of light intensity. Second, G. frondosa were cultivated at four CO2 level, 500, 800, 1000, and 1500 ppm after spawning. Comparison in production of fruit body in G. frondosa better result were obtained at lower CO2 levels below 800 ppm. The higher ratio of primordia formation, faster time of primordia initiation and days to harvest(57 days), higher yields and quality of fruit body at lower CO2 levels below 800 ppm than higher that over 1000 ppm, whereas shape of fruit body at higher CO2 levels over 1000 ppm were showed abnormal and lower quality.

This study was carried out to obtain basic data on physiological characteristics for an artificial cultivation of Grifola frondosa. Ten strains of G. frondosa were collected from Korea, China and Japan and those were isolated and tested. PDA medium was selected for the favorable culture medium. The optimal conditions for the mycelial growth of G. frondosa were 25℃ and pH 4∼5 respectively. The carbon and nitrogen sources for the optimum mycelial growth were fructose and peptone, respectively. Highest mycelial growth was observed when the C/N ratio was 10∼20. Significant differences among lines were found for production yield and crop cycle time. KME44009 had a short spawn run time, high yield, good color and quality compare with other strains. To find out the suitable medium composition of G. frondosa, physicochemical conditions were investigated. The T2 formulation (55:25:8:12 mixing ratio of oak sawdust, oak chip, wheat bran and soybean cake) showed the shortest time to complete the crop cycle and the highest yields(weight of fresh mushrooms harvested at maturity). Among the physicochemical factors, pH, crude oil and total nitrogen may affect mushroom yield. Therefore, development of suitable medium would benefit from increased production efficiency of G. frondosa mushrooms and be commercial potential.

This study was carried out to obtain basic data on physiological characteristics for an artificial cultivation of Grifola frondosa. Ten strains of G. frondosa were collected from Korea, China and Japan and those were isolated and tested. PDA medium was selected for the favorable culture medium. The optimal conditions for the mycelial growth of G. frondosa were 25℃ and pH 4～5 respectively. The carbon and nitrogen sources for the optimum mycelial growth were fructose and peptone, respectively. Highest mycelial growth was observed when the C/N ratio was 10～20. Significant differences among lines were found for production yield and crop cycle time. KME44009 had a short spawn run time, high yield, good color and quality compare with other strains. To find out the suitable medium composition of G. frondosa, physicochemical conditions were investigated. The T2 formulation(55:25:8:12 mixing ratio of oak sawdust, oak chip, wheat bran and soybean cake) showed the shortest time to complete the crop cycle and the highest yields(weight of fresh mushrooms harvested at maturity). Among the physicochemical factors, pH, crude oil and total nitrogen may affect mushroom yield. Therefore, development of suitable medium would benefit from increased production efficiency of G. frondosa mushrooms and be commercial potential.