본 연구에서는 홍삼음료의 다양화 및 기능성 강화와 여러 기능성을 가진 황기의 식품으로의 활용에 역점을 두고, 기존의 증점제를 대신하여 동결건조 천년초열매를 첨가한 새로운 기능성 홍삼음료을 개발하고자 하였다. 먼저 높은 항산화활성을 지닌 황기추출물을 얻고자 추출용매의 지용성정도를 달리하면서(100(증류수) : 0(95% 에탄올), 75 : 25, 50 : 50, 25 : 75) 80oC에서 추출한 추출물의 항산화활성을 측정한 결과, ABTS 및 DPPH radical 소거능 실험에서 증류수의 비율이 증가할수록 추출물의 항산화력이 증가하는 것으로 나타났다. 추출용매의 온도에 의한 황기추출물의 항산화활성 차이를 알아보고자 추출용액의 온도를 달리하면서(60, 80oC) 추출한 추출물의 항산화활성을 비교해 본 결과, 80oC에서의 추출물이 증류수와 에탄올혼합비율과는 상관없이 대부분 60oC 추출물보다 높은 값을 나타내었다. 기존의 점증제를 대신하여 다양한 기능성을 함유한 천연물 소재인 천년초분말을 선택하였으며, 구아검용액과 농도별 점도를 비교해 본 결과, 구아검 0.1%(w/v) 용액과 비슷한 점도를 나타내기 위해서는 천년초분말의 경우 1-2%(w/v)의 농도가 필요한 것으로 분석되었다. 황기와 천년초 첨가가 홍삼용액의 저장성(35oC)에 미치는 영향을 조사하기 위하여 이들 용액의 pH와 총미생물수를 7일 동안 측정하였다. 홍삼용액에 황기와 천년초를 첨가한 경우, 저장 1일째부터 pH가 급격히 감소하기 시작하였으며, 저장 3일째에는 pH가 약 3.6까지 떨어진 다음, 저장 6일 이후에는 다소 pH가 증가하였으며, 천년초분말을 첨가한 용액에서는 황기의 농도증가에 의한 유의적 pH 감소는 나타나지 않았다. 천년초를 첨가한 홍삼용액의 경우 황기의 농도와는 상관없이 저장 7일째 미생물의 성장을 관찰할 수 없었으며, 천년초를 첨가하지 않은 시료에 비해 6 log cycle 이상의 큰 살균효과를 나타내었다. 황기(3, 5%, w/v), 천년초(1.2%, w/v)를 함유한 홍삼용액(5%, w/v)이 대장암 및 뇌종양세포 증식에 미치는 영향을 각각 0.5 mg/mL과 1 mg/mL의 농도에서 살펴본 결과, 종양세포에 대한 의미 있는 증식억제효과는 나타나지 않았다.

A study was conducted to determine contamination status of fish sold at wholesale market in Seoul. A total of 79 samples (35 different kindry fish) were collected from the wholesale market. E. coli and coliform group bacteria were cultured and tested for sensitivity against antibiotics. The results are summarized as follows; 1. E. coli was isolated from 23 out of 79 samples (29.1%), and coliform groups from 53 out of 79 (67.1%). 2. Of coliform group, Citrobacter freundii was the most common and Enterobacter cloacae was the next. 3. 23 E. coli strains isolated from fishes were resistant to Oxacillin, Erythromycin and Lincomycin, meanwhile 23 E. coli strains were sensitive to Cefoperazone, Ceftazidime, Imipenem, and Ciprofloxacin.

In this lysimeter experiment, temporal changes of water percolation rate, irrigation requirement and No3 －-N leaching were investigated under different cultural practices that were no-till direct seeding on flooded paddy (NTDSF), till direct seeding on flooded paddy (TDSF), and transplanting. The highest water percolation rate of 3,001 l/m2 was measured in NTDSF. Others were 2,551 l/m2 and 2,210 l/m2 in TDSF and transplanting. Water percolation rate in NTDSF and TDSF was increased by 36% and 15% compared to transplanting. Water percolation rates in all cultural practices were increased remarkably from the reproductive growth stage and relatively large amount of water loss through percolation was measured even after the reproductive growth stage. A total irrigation requirement was 3,469 l/m2 in NTDSF and 2,898 l/m2 in TDSF. That was equivalent to 45% and 21 % of increase compared to 2,389 l/m2 in transplanting. The largest No3 －-N leaching through the entire rice growing period was 701 mg/m2 in NTDSF and was followed by 494 mg/m2 in TDSF and 465 mg/m2 in transplanting. The ratios to the total amount of No3 －-N leaching at the vegetative growth stage, reproductive growth stage and ripening stage were 31 %, 41 % and 28% in NTDSF; 21 %, 48% and 31 % in TDSF; and 18%, 48% and 35 % in transplanting.

In this study, the Dilution Method is used to measure river discharge through the hydraulic model test. the dilution method is divided into Constant-Rate-Injection Method and Slug-Injection Method in the river discharge measurement techniques. When the dilution method is applied in the hydraulic model flume, it is analyzied that the estimated error of constant-rate-injection method is less than that of the slug-injection method, and the result shows that floodflow analysis is more efficient than lowflow analysis as compared observed discharge with calculated discharge. The result of statistical error analysis shows that the constant-rate-injection method is appropriate technique for the measurement of the river discharge. Therefore, the dilution method among the river discharge measurement techniques can be applied for the river basin which can`t be measured with current meter or unsteady-flow regime in the urban-small drainage or hydraulic structure equipment area and can be obtained more exact results than any other discharge measurement techniques.

This study is aimed at the development of the adequate sediment yield formulas in Wi-Stream basin ; IHP representative basin in Korea. As a result of applying outstanding sediment yield formulas among the existing formulas. It is analyzed that including the Engelund & Hansen formula, Yang formula is proper to the Wi-Stream basin. And as a result of sensitivity analysis to the sediment parameters it is analyzed that Rijn and Ackers & White formula is more sensible than any other formulas which has been applied the velocity and depth among the parameters. Also, Engelund & Hansen and Yang formula is less sensible than any other formulas. In Wi-Stream basin, it is analyzed that Yang and Engelund & Hansen formula is the most suitable sediment yield formula in this study. But because the existing formulas had been developed in foreign countries and applied the foreign natural rivers and reservoirs, it makes careful use of Korean alluvial river and hoped that it will be developed the most adequate formula in Wi-Stream basin.

The objectives of this study is to evaluate the total runoff yield, peak flow and peak flow travel time depending on the urbanization, return period and rainfall patterns at the downstream of Manchon urban watershed in TaeGu City. SWMM(Storm Water Management Model) is used for runoff analysis based on 5 different steps of urbanization and 4 different types of Huff`s quartile according to 8 return periods. It is analyzed that the order of total runoff yield according to rainfall patterns is Huff`s 4, Huff`s 2. Huff`s 3 and Huff`s 1 quartile, that of peak flow magnitude is Huff`s 2, Huff`s 1, Huffs`4 and Huff`s 3 quartile at present development ratio. under the 60, 70, 80 and 90% of urbanization to the 50% of urbanization by means of the rainfall patterns, the mean increasing ratio of total runoff yield for each case is 4.55, 11.43, 16.07 and 20.02%, that of peak flow is 5.82, 13.61, 17.15 and 18.83%, the mean decreasing ratio of peak flow travel time is 0.00, 2.44, 5.07, and 6.26%, the mean increasing ratio of runoff depth is 4.51, 11.42, 16.02 and 20.05%, respectively. the mean increasing ratio of total runoff yield by means of each return period & Huff`s 3 quartile is 4.86, 12.11, 17.14 and 21.56%, that of peak flow is 5.89, 13.97, 17.82 and 19.71%. Therefore, as the result of this study, it can be used for principal data as to storm sewage treatment and flood damage protection planning in urban small watershed.