콜린은 정상적인 세포벽의 기능, 아세틸콜린의 합성, 지질의 운송, 그리고 매칠기의 공급원으로서 중요한 유기화합물이다. 콜린의 보충이 콜린성 신경세포의 활성을 통해 학습 및 기억력 향상에 효과가 있다는 연구 보고를 토대로, 시중에서 시판되는 많은 우유제품 속에 콜린 함유량을 증가시켜 뇌를 좋게 하는 기능성 식품으로 판매되고 있다. 따라서 본 연구에서는 효소측정법을 이용하여, 시중에서 판매되고 있는 전유와 첨가우유들을 5개 회사의 제품을 대상으로 콜린의 함량을 분석하였다. 콜린의 표준용액을 이용하여 구한 표준곡선은 0.00316의 기울기와 0.998의 r2값을 나타내었다. 회수율은 89.8-97.6 %로 양호한 값을 나타내었다. 분석한 우유에서의 콜린함유량은, 전유에서는 14.56-15.19 mg/100 g이었고, 첨가우유에서는 4.11-11.50 mg/100 g을 나타내었다.

Molecular markers are useful for selecting to include superior character genetic like as strong immune system and rapid growth in fish. The marker is also very important part of breeding technology in Olive flounder (Paralichthys olivaceus). Single nucleotide polymorphisms (SNPs) marker is already in use widely for genomic research and breeding. But this SNPs marker hardly has been validated for screening functional genes in Olive flounder. We study identify single nucleotide polymorphisms (SNPs) on Expressed sequence tag (EST) database, develop usable SNP marker and apply to wild sample and cultured of olive flounder. As a result, Out of total 4.327 ESTs, 693contigs and 514 SNP from total contigs were detected while these substitutions include 297 transitions and 217 transversions. 144 developed markers were applied in 16 samples (wild 8, culture 8), Out of total marker, only 32 markers had detected polymorphic in sample. Polymorphism of 32 markers was observed in the variety genes region involved in immunity and protein synthesis. And the 32 marker were identified 21 transitions, 11 transversions, and indel was not detected in polymorphic SNPs. The analysis on heterozygosity by sample showed 0.34 in wild sample and 0.29 in cultured sample. In conclusion, we was identified SNP and Polymorphism by designed new marker, it supports that development marker is suitable for SNP detection and diversity analysis in Olive flounder. The outcome of this study can be basic data for researches for immunity gene and characteristic with SNP.

Ovarian tissue cryopreservation and transplantation causes follicle depletion. To overcome this problem, we investigate the effect of Anti-Müllerian hormone (AMH), a follicle recruitment control hormone, supplementation before and/or after mouse ovarian transplantation. A total of 120 5-week-aged BD F-1 female mice were used. The mice were randomly divided into four groups according to AMH doses (0, 5, 25, 125 μg/mL, respectively). AMH was injected intraperitoneally on every other day for a week before, after, or before and after transplantation of ovaries under kidney capsules was performed. One week after transplantation, follicular normality was evaluated by histological analysis and TUNEL assay. In Group A and C, morphologically intact follicle (G1) ratios of AMH treated groups showed no statistically significant difference. In Group B, G1 ratios of 25 and 125 μg/mL of AMH treated groups were higher than those of 5 μg/mL treated group, but there was no improvement in G1 ratio after AMH treatment. In every group, apoptotic follicle ratios did not show any trend according to AMH treatment. Proportions of primordial follicle were not significantly different according to AMH treatment in all groups. The result of the present study demonstrated that AMH treatment during on transplantation of cryopreserved ovaries has no significant effect on follicle survival and prevention of follicle depletion.

Objective : To investigate the effects of Simvastatin and Methylprednisolone on ovarian tissue cryopreservation and transplantation using mouse models. Methods : The mice were randomly distributed into 1 control and 3 experimental groups. The B6D2F1 mice were given oral Simvastatin (5 mg/kg), intravenous Methylprednisolone (15 mg/kg), or a combination of both at 2 hours before ovariectomy. Same volume of normal saline was given perorally in the control group at 2 hours before ovariectomy. The ovarian tissues were vitrified accrording to our protocols. The vitrified ovaries were warmed 1 week later and auto-transplanted under bilateral kidney capsules. The ovaries and blood sera were collected at 2, 7 or 21 days after transplantation. Histological analysis, TUNEL assay, immuno-histochemistry for CD31, serum AMH level and embryonic development after in vitro fertilization were assessed for evaluation. Results : With regard to the total grade 1 follicle rate, both Simvastatin or Methylprednisolone treated groups were significantly increased at 2, 7 or 21 days after transplantation (except Simvastatin treated group at 7 days). A combination of Simvastatin and Methylprednisolone group was significantly improved in terms of the total G1 follicle rate, apoptotic follicle rate, CD31 positive area and serum AMH after ovarian tissue transplantation. However, there were no statistically difference with respect to the oocyte maturation rate, blastulation rate, and the other embryonic development parameters after in vitro fertilization procedure among the four groups. Conclusion : Our results suggest that combined donor Simvastatin and Methylprednisolone have beneficial effects on the quality and function of transplanted ovarian tissues.

“Daeshin” (Perilla frutescens (L.) Britton), a new cultivar for leaf vegetable, was developed from a cross between YPL5 (Ipdeulkkae1/YCPL187) and Milyang2 at the National Yeongnam Agricultural Experiment Station (NYAES), RDA, in 2002. For the cultivation of leaf vegetable perilla, seeds are sown in narrow spacing of 10 × 5 cm and all branches are removed to harvest uniform leaves from main stem. Because of the narrow planting distance, it is difficult to distinguish seeds from soil in dark brown seed cultivar. Therefore the gray-white size color of “Daeshin” is very useful characteristic for easier sawing in perilla cultivation. “Daeshin” grow vigorously and yield high quality leaf. The fresh leaf yield of “Daeshin” is 9% higher than that of “Ipdeulkkae 1” (4,823 vs. 4,459 kg/10a). For the leaf production, “Daeshin” is adaptable to whole arable land in South Korea. However, the southern parts of Korea such as South Gyeongsang and South Jeolla provinces are appropriate for the seed production.