This research assessed the disparity in anion generation according to the vertical structure of a Zelkova Serrata tree for the purpose of creating a pleasant and green city environment. Measurements for the study were conducted between July and August of 2014 in Chung-ju in the central region of the Republic of Korea. The average anion generation of vertical structure trees during active photosynthesis periods was: L Section (839.0 ea/cm3) > M Section (664.6 ea/cm3) > U Section (472.0 ea/cm3). According to DMRT analysis, significant difference was found in the average between the L, or M Locations and the U Locations. During dormant photosynthesis periods, records showed that the anion production at the M Location (1,212.5 ea/cm3) > L Location (1,050.4 ea/cm3) > H Location (844.1 ea/cm3), According to DMRT analysis, the difference within each location was significant for α=0.05. In a comprehensive analysis of the weather factors in each vertical structure, anion generation during active photosynthesis periods showed a positive correlation with solar radiation and a negative correlation with wind speed. Dormant photosynthesis periods showed negative correlations with both solar radiation and temperature, and positive correlations with relative humidity and wind speed. Predictions from a multicenter retrospective study showed that during active photosynthesis periods, Y1 = 282.443X1 + 512.07, and Y2 = 314.337X1 + 16.913X2, while during dormant photosynthesis periods, Y1=391.009X1 + 840.043, and Y2=351.412X1 + 32.765X2.