PURPOSES: This study analyzed the lane-by-lane traffic flow characteristics in Korea by using real-world data, including congestion levels, for 2-, 3-, and 4-lane freeways. METHODS : On the basis of a literature review, lane flow and speed characteristics were analyzed using flow measurements and speed ratios. In addition, the effect of congestion levels on traffic flow were visualized using rescaled cumulative plots. RESULTS: Driver behavior varied depending on the congestion level. During free-flow conditions, the lane-use ratio of individual lanes varied largely, whereas during congestion, the ratio was nearly the same for all lanes (i.e., equilibrium). During maximum-flow and congestion conditions, the median lane was used more than the shoulder lane, whereas during all other conditions, the shoulder lane had a higher lane-use ratio. In 3- or 4-lane freeways, the lane-use ratio of the median lane always exceeded 1 and was the highest during free-flow conditions. CONCLUSIONS : The results of the present analysis can be used as an index to predict congestion before a lane is overcapacitated. Moreover, the results can be applied in variable lane guidance systems, such as car navigation systems and variable message displays, to control traffic flow.