The anomalous behaviour of animals prior to a large earthquake has been frequently reported from many places throughout the world since ancient times. This study is to experimentally testify what relations exist between the anomalous animal behavior and electric field effect, which is reported due to the piezo-electric effects in a basement rock before earthquake occurrences. We investigated the electric field effects on behaviors of two kinds of rats and birds. Rats show more sensitive anomalous behavior than birds. Even though the current applied to the experimental birds and rats is just a few μA, they show various abnormal behaviors. The anomalous animal behaviors under the small ground electric field may have some relations to the actual phenomena before great earthquake.