몇 종의 주요 농작물 해충에 대한 식물추출물(cinnamon extract 5% + citronella oil 10% + citrus oil 30% + derris extract 10% + neem extract 20% + penetrating surfactant 25%)의 살란 효과를 분무법으로 검정하였다. 노린재류의 경우 알이 부화하는 과정에서 치사하는 경향을 보였다. 식물추출물(500배액) 처리시 썩덩나무노린재(Halyomorpha halys), 톱다리개미허리노린재(Riptortus clavatus), 홍비단노린재(Eurydema dominulus), 온실가루이(Trialeurodes vaprarorium), 담배가루이(Bemisia tabaci), 파밤나방(Spodoptera exigua), 검거세미밤나방(Agrotis ipsilon) 에 대한 최종 살란 효과는 100%로 높게 나타나, 향후 산업화가 가능할 것으로 생각된다.

시설 고추 주요 해충인 꽃노랑총채벌레(Frankliniella occidentalis)의 방제를 위해 유기농업자재들의 단독 처리 및 종합 투입시 방제 효과를 검정 하였다. 황색끈끈이롤트랩은 지제부에 설치하고, 포식성 천적(Hypoaspis miles)은 지제부에 접종하며, 식물추출물(커스터드애플시드 오일 50% + 계 피 추출물 10%)은 경엽살포하는 방식으로써 각각의 단독 처리시 방제 효과는 45.3, 36.6, 50.2%였다. 한편, 3종의 유기농업자재들을 종합 투입했을 경우 고추 수확기 방제 효과가 무처리 대비 62.4~80.9%로 유지되어, 시설 고추 꽃노랑총채벌레 친환경 방제용으로 활용이 가능할 것으로 생각된다.

In this study, Bacillus velesensis TJS119, isolated from freshwater, demonstrated growth inhibition against insect pathogenic fungi. The culture medium of the B. velezensis TJS119 strain underwent sequential fractionation with n-hexane, chloroform, ethyl acetate, n-butanol, and water. Notably, the n-butanol fraction exhibited significant antifungal activity against Metarhizium anisopliae and Beauveria bassiana. LC/MS analysis of antifungal peaks identified the production of various lipopeptides by B. velezensis TJS119, including two types of iturin A (C14, C15), four types of fengycin A (C14, C15, C16, C17), and two types of fengycin B (C16, C17). The antifungal efficacy of Iturin A and Fengycin against insect pathogenic fungi was further validated using the paper disc diffusion method. These findings underscore the potential of B. velezensis TJS119 as a promising candidate for future research and applications in the realm of agricultural biological controls against fungal diseases.

One of cosmopolitan pest, Agrotis ipsilon, causes serious economic damages in horticultural crops. This study compared the host fitness of A. ipsilon among nine major horticultural crops in Korea. Among the nine crops, the population of A. ipsilon failed to complete its development in spinach, cucumber, melon, and kidney bean. The host effects on development and reproduction of A. ipsilon were further investigated in the remained five crops. Host plants significantly (P < 0.05) affected the development-related factors of A. ipsilon eggs, larvae, and pupae. They also affected the adult reproduction-related factors including preoviposition period, oviposition period and number, and longevity except for the prepupa stage. A positive relationship was found be tween biological factors. Among the nine crops in this study, napa cabbage showed the highest suitability for the A. ipsilon populations. These findings in this study would be helpful to understand the ecology and develop the man agement tactics of A. ipsilon in horticultural crops.

농가 현장에서는 미소 해충의 약제저항성 발달로 인하여 방제에 어려움을 겪고 있다. 본 연구는 약제저항성 수준을 효율적으로 진단하고, 화학적 방제 의사결정을 지원할 수 있는 약제저항성 관리 플랫폼을 구축하고자 수행하였다. 플랫폼은 크게 ⅰ) 농가 맞춤형 약제 추천, ⅱ) 지역별 약제저항성 지도로 구성되어 있다. 이용자는 잔류접촉법 기반의 생물검정법 RCVpW(Residual Contact Vial plus Water) 결과를 현장에서 입력하면 PLS에 부합 한 약제 정보를 제공받을 수 있다. 또한 생물검정 결과는 DB화를 통해 지역별 약제 저항성 정보 지도를 보여줌으 로써 해충별, 작물별, 연도별 저항성 패턴 결과를 확인할 수 있다. 본 플랫폼을 활용한다면 약제의 오남용을 줄임 은 물론 해충의 약제저항성 발달 지연 및 약제저항성 발달 양상을 확인할 수 있어 향후 방제전략 수립에 활용할 수 있을 것으로 생각된다.

Honey bees are crucial pollinators for agricultural and natural ecosystems, but are experiencing heavy mortality in Korea due to a complex suite of factors. Extreme winter losses of honey bee colonies are a major threat to beekeeping but the combinations of factors underlying colony loss remain debatable. Finding solutions involves knowing the factors associated with high loss rates. To investigate whether loss rates are related to Varroa control and climate condition, we surveyed beekeepers in korea after wintering (2021–2022 to 2022–2023). The results show an average colony loss rate of 46%(2022) and 17%(2023), but over 40% colony loss before wintering at 2022. Beekeepers attempt to manage their honey bee colonies in ways that optimize colony health. Disentangling the impact of management from other variables affecting colony health is complicated by the diversity of practices used and difficulties handling typically complex and incomplete observational datasets. We propose a method to 1) Varroa mite population Control by several methods , and 2) Many nursing bee put in hive before wintering.

This study investigated ethopabate (EPB) residues in edible tissues of broiler chickens given in drinking water and established the withdrawal time (WT) of EPB in poultry tissues. Twenty-four healthy Ross broiler chickens were orally administered with EPB at the concentration of 3.8 mg/L for 14 days (EPB-1, n=24) and 15.2 mg/L for 7 days (EPB-2, n=24) through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3, and 5 days, respectively. EPB residue concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient values ranged from 0.9980 to 0.9998, and the limits of detection and quantification (LOQ) were 0.03~0.09 and 0.1~0.3 μg/kg, respectively. Mean recoveries in muscle, liver, kidney and skin/fat tissues were 95.9~109.8, 108.7~115.3, 89.9~96.6 and 86.7~96.8%, respectively, and coefficient of variations were less than 17.11%. At the end of the drug-administration period (0 day), EPB was detected at levels under the LOQ in all tissues from both the EPB-1 and EPB-2 groups. According to the results of EPB residue in Ross broiler tissues, withdrawal periods of both EPB-1 and EPB-2 in poultry tissues were established to 0 day. In conclusion, the developed analytical method is suitable for the detection of EPB in poultry tissues, and the estimated WT of EPB in poultry tissues will contribute to ensuring the safety of Ross broiler chickens.

From 2020, Korean Animal and Plant Quarantine Agency has reset the withdrawal time (WT) for veterinary drugs typically used in livestock in preparation for the introduction of positive list system (PLS) program in 2024. This study was conducted to reset the MRL for amprolium (APL) in broiler chickens as a part of PLS program introduction. Forty-eight healthy Ross broiler chickens were orally administered with APL at the concentration of 60 mg/L (APL-1, n=24) for 14 days and 240 mg/L (APL-2, n=24) for 7 days through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3 and 5 days, respectively. Residual APL concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 0.3~5.0 μg/kg), recoveries (81.5~112.4%), and coefficient of variations (<15.5%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In APL-1, APL in all tissues except for kidney was detected less than LOQ at 3 days after drug treatment. In APL-2, APL in liver and kidney was detected more than LOQ at 5 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal periods of APL-1 and APL-2 in poultry tissues were established to 3 and 2 days, respectively. In conclusion, the developed analytical method is sensitive and reliable for detecting APL in poultry tissues. The estimated WT of APL in poultry tissues is longer than the current WT recommendation of 2 days for APL in broiler chickens.

돌발성 해충인 매미나방의 방제체계 수립을 위해 시판 유기농업자재 21종에 대한 살충 활성을 실내 검정하였다. 90% 이상의 살충 활성을 보인 유기농업자재 11종을 선발하였으며, 유효성분들을 분석하였다. PLS 제도의 대응하여 본 연구 결과는 돌발성 매미나방의 친환경 방제 및 향후 유기농업자재의 개발에도 활용이 가능할 것으로 생각된다.

본 연구는 콜레마니진디벌의 품질확인장치에 적용할 수 있는 LED 광원을 선발하기 위하여 수행되었다. 4개의 LED 광원(385, 405, 450, 및 660 nm)을 대조구로 5,000k 백색 LED를 사용하여 Y자관 시험에서 선택시험을 수행한 결과, 385 nm LED 광원만 콜레마니진디벌에 대한 유인효과가 있었다. 하지만 3개의 LED 광원(385, 405, 및 450 nm)과 대조구로 5,000k 백색 LED를 사용하여 비선택시험을 수행한 결과, 450 nm > 385 nm > 405 nm 광원 순으로 유인효과가 높았으나 450 nm와 385 nm 광원간 유의차는 없었다. UV-A에 속하는 385 nm LED 광원은 피부질환을 초래할 수 있는 우려가 있어 제외하였고, 최종 450 nm LED 광원을 콜레마니진디벌 품질확인장치에 적용할 광원으로 선발하였다. 450 nm LED 광원을 콜레마니진디벌 우화기구에 적용한 결과, 87.4%의 높은 유인효과를 확인 할 수 있었다. 이러한 결과는 천적 품질관리에 유 용하게 활용될 수 있을 것으로 생각된다.

시금치의 주요 해충인 흰띠명나방(Spoladea recurvalis) 유충의 살충제 5종에 대한 감수성을 검정하였다. Lufenuron EC, chromafenozide EC, chlorantraniliprole WP, tebufenozide WP, pyridalyl EW는 각각 2(12.5 ppm), 4(12.5 ppm), 8(2.5 ppm), 4(20.0 ppm), 8(12.5 ppm)배 의 희석농도에서 90% 이상의 높은 살충활성을 보였다. 추천농도로 경엽처리 후 7일이 경과된 시금치 잎에 흰띠명나방 유충이 72시간 동안 노출되 었을 경우 chromafenozide EC, chlorantraniliprole WP, tebufenozide WP, pyridalyl EW의 살충률은 각각 98.3%, 100%, 95.0%, 100%로 나타나 높은 잔효성을 보였다. 흰띠명나방에 대한 방제효과를 2개소(화성, 연천)에서 포장검정 결과, 5종의 약제 모두 2개소에서 약제처리 7일 경과 후 90% 이상의 방제효과를 보였으며 2배량에서도 약해가 없어 향후 흰띠명나방 방제약제로 시금치에 활용이 가능할 것으로 판단된다.