Hydrogen isotopes (H, D, T) separation technologies have received great interest for treatments of tritiated liquid waste produced in Fukushima. In addition, the separated deuterium and tritium can be utilized in various industries such as semiconductors and nuclear fusion as expensive and rare resources. However, separating hydrogen isotopes in gas and liquid forms still requires energyintensive processes. To improve efficiency and performance of hydrogen isotope separation, we are developing water electrolysis, cryosorption, distillation, isotope exchange, and hydrophobic catalyst technologies. Furthermore, an analytical method is studied to evaluate the separation of hydrogen isotopes. This presentation introduces the current status of hydrogen isotope research in this research group.