Seedling drought resistance of three alfalfa cultivars-Medicago sativa L. cv. Aohan, Medicago sativa L. cv. Zhaodong and Medicago sativa L. cv. Tumu No.2 were studied and evaluated. The permeability of plasma membrane, the free proline content and the peroxidase (POD) activity of three alfalfa cultivars were tested at 24h after treating with 20% polyethylene glycol (PEG-6000). The results indicated t㏊t the drought resistance ability of all these varieties was in the order of Medicago sativa L. cv. Aohan, Medicago sativa L. cv. Zhaodong and Medicago sativa L. cv. Tumu No.2.