본 연구에서는 인천광역시에서 유통 중인 한약재의 안 전성을 확인하기 위해 24품목의 50건을 대상으로 345종 의 잔류농약을 분석하였다. 잔류농약은 미량으로도 인체 의 건강에 해를 가할 수 있는 유해 물질로 주의가 필요하 다. 기준 규격외 다양한 잔류농약 확인을 위해, QuEChERS 법으로 전처리후 분석한 결과, 7품목 24건(48%)의 한약재 에서 총 22종의 잔류농약이 검출되었으며, 농약이 검출된 한약재는 천궁, 택사, 당귀, 백지 순으로 많았다. 검출 농 약 중 2건을 제외하고는 모두 기준이 설정되어 있지 않은 농약이었으나, 위해 평가 결과 안전한 수준으로 확인되었 다. 또한, 동일 품목에서 다빈도로 검출되는 농약을 확인 하여, 해당 품목의 지속적인 잔류농약 모니터링의 필요성 을 시사하였으며, 대한약전의 전처리법에 따른 비교분석 에서도 동일한 농약이 검출되어, QuEChERS법을 이용한 생약의 잔류농약 분석 가능성을 제시하였다. 결과적으로, 소비자 안전을 위해서 기준규격 잔류농약 항목 외에도, 생 약에 대한 지속적인 잔류농약 모니터링과 신뢰성 높은 고 효율 분석법 개발 연구가 계속 필요할 것이다.

PURPOSES : Under the Traffic Safety Act, the installation and management of transportation facilities (facilities and attachments necessary for the operation of transportation, such as roads, railways, and terminals) must take necessary measures to ensure traffic safety, such as enhancing safety facilities. Recently, railway operators have graded the congestion level inside railway stations and vehicles, addressing safety and convenience issues arising from congestion and providing this information to users. However, for bus-related transportation facilities (such as bus stops, terminals, and transfer facilities), criteria and related research for assessing traffic congestion are lacking. Therefore, this study developed a model for the congestion risk factors of four bus-related transportation facilities and proposed criteria for classifying congestion risk levels. METHODS : This study involved selecting congestion risk influence variables for each traffic facility through field surveys, calculating congestion risk index values through evacuation and pedestrian simulations, and constructing a congestion risk influence model based on the ridge model. RESULTS : The factors influencing congestion were selected to include the number of people waiting, effective sidewalk width, and number of bus stops. As a result of developing congestion risk grades, the central bus stops were determined to be in a severe stage if the Average Waiting Time (AWT) was 2.7 or above. Roadside bus stops were considered severe at 4.2, underground metropolitan transit centers at 3.7, and bus terminals at 5.9 or above. CONCLUSIONS : This study can help establish a foundation for a safety management system for congested areas in transportation facilities. When the congestion risk prediction results correspond to cautionary or severe levels, measures that can reduce congestion risk must be applied to ensure the safety of road users.

2023년에 국내에서 보고된 적이 없던 외래흰개미 3종이 각각 인천, 서울, 창원에서 발견되었다. 종 동정 결과, 인천에서 출현한 종(Coptotermes gestroi)은 Subterranean termite로 밝혀졌으며, 서울과 창원에서 출현한 종 (Cryptotermes domesticus, Incisitermes minor)은 Drywood termite로 밝혀졌다. 본래 우리나라에는 Subterranean termite 흰개미가 2종이 분포하고 있다고 보고되어 있었으나, 새로운 과 또는 속의 외래흰개미가 발견되면서 국민 들의 우려가 커지고 있다. 국내에서는 일부 외래흰개미를 법적으로 지정(유입주의 생물 2종, 관리병해충 15종)하 여 관리하고 있는데, 해당 흰개미와 그 외의 신규 흰개미의 출현으로 인한 피해가 우려되는 상황이다. 본 연구에서 는 ‘23년 유입된 3종 및 기존 법정관리 외래흰개미, 그리고 국내 유입과 위해가 우려되는 외래흰개미 5과 (Archotermopsidae, Kalotermitidae, Rhinotermitidae, Termitidae, Termopsidae)를 조사하였으며, 이들의 생태적 특 징과 외국의 사례를 통해 외래흰개미 대비 방법에 대하여 고찰하였다.

전 세계적으로 RNA 간섭(RNA interference, RNAi)을 활용한 해충방제제 연구가 활발히 진행되고 있다. 대표적 으로 Monsanto의 서부 옥수수 뿌리벌레(Diabrotica virgifera virgifera) 특이적 방제용 dsRNA (DvSNF7)를 발현하 는 옥수수 종자가 상용화 되었고, 2016년 이 종자가 국내 사료 및 식품용으로 수입이 승인 되었다. 본 연구는 국내 에 서식하는 좁은가슴잎벌레(Phaedon brassicae)를 Non-target 곤충으로 사용하여, 옥수수 종자에 사용된 DvSNF7 dsRNA의 잠재적 위해성을 평가했다. P. brassicae의 SNF7 유전자와 DvSNF7 dsRNA 간 Sequence유사성 을 확인했다. 다음으로 P. brassicae가 DvSNF7 dsRNA를 섭식할 수 있는 환경을 조성하여, 치사 효과 실험과 Sequence특이적인 Knockdown효과를 확인하였다. 그 결과, DvSNF7 dsRNA는 P. brassicae생존에 영향을 미치지 않았으며, P. brassicae의 SNF7유전자를 Knockdown시키지 않음을 확인했다. 이번 연구를 통해 D. virgifera virgifera와 같은 과인 P. brassicae 는 SNF7유전자 간 Sequence유사성이 있지만, Sequence특이성이 부족하여 생존과 유전자 발현에 영향을 주지 않음으로써 위해성이 낮음을 확인했다.

As climate changes and global trade volume increases, the spread of invasive alien species accelerates. Early prevention before occurrence is crucial for invasive pest control. Therefore, this study modeled the current and future potential distribution of the tomato leafminer(Tuta absoluta) (Meyrick) (Lepidoptera: Gelechiidae), the most significant pest affecting tomatoes, in Korea. This pest primarily feeds on Solanaceae crops and can cause extensive damage, resulting in 50-100% loss of crops in greenhouses or fields. While previously unreported in Korea, it invaded China in 2017, indicating a potential threat to Korea. The potential distribution of the tomato leafminer in Korea under current and three future climate scenarios (SSP1-26, SSP3-70, SSP5-85) was predicted using the MaxEnt model. Additionally, elevation and land cover were incorporated as abiotic factors considering the ecological characteristics of the pest.

Abstract Purpose : To investigate the prevalence and risk factors for dry eye syndrome (DES) in university students. Methods : A cross-sectional survey of university students was conducted through a combination of online smartphone surveys using Google Forms and face-to-face questionnaires. The questionnaire consisted of questions about risk factors for dry eye disease and the 5-item Dry Eye Questionnaire (DEQ-5). Results : A total of 288 university students participated in the study. The average age was 22.5±2.1 years, w ith 54.2% female a nd 4 5.8% m ale. The m ean of t he D EQ-5 s core w as 7.37±4.65, and the prevalence of DES by DEQ-5 was 47.2%. Female gender (p=0.012), digital screen use for more than 8 hours per day (p=0.018), digital screen use for more than two hours without a break (p=0.035), contact lens wear (p=0.016), refractive surgery (p=0.020), less than 6 hours of sleep per night (p=0.0169, and allergic conjunctivitis (p=0.016) were relevant associated risk factors for DES. Conclusion : The prevalence of DES in young adults may be underestimated in comparison to middle-aged and older adults. The severity of DES depends on several factors, including genetic and environmental factors, so identifying risk factors would help with proper prevention and early diagnosis. Key words : DEQ-5, Dry eye syndrome, Risk factor

In recent years, deaths and missing people have continuously occurred due to man overboard (MOB) and suicide on passenger ships. However, due to the complex deck structure and enlargement of passenger ships, closed-circuit television (CCTV) coverage is limited, and it is difficult to prevent accidents for all passengers with limited crews. Therefore, a real-time system for detecting high-risk blind spots on passenger ships is needed through risk analysis. This study used a combination of the following three risk factors to calculate and evaluate the risk of MOB and suicide by deck area of the passenger ship: 1) distance away from guard rails, 2) the visibility of CCTV, and 3) ship operating conditions. Based on the survey from experts, risk scores of MOB and suicide accidents by deck area on a passenger ship were yielded.

Captive breeding and reintroduction are crucial strategies for conserving endangered species populations. However, fish raised in predator-free environments, show a lack of recognition of predationrelated stimuli such as chemical and visual signals. It is critical to recognize chemical signals from injured conspecifics, also known as alarm signals, and the order or shape of predators to indicate the spread of predation risk in the habitat. We conducted a laboratory experiment to determine and adjust the optimal exposure period to induce appropriate anti-predator behavior response to different types of stimuli (Chemical, Visual and Chemical+Visual) for the endangered species Microphysogobio rapidus. Our results demonstrate that predator avoidance behavior varies depending on the types of stimuli and the duration of predation risk exposure. First, the results showed captive-breed M. rapidus show lack of response against conspecific alarm signal (Chemical cue) before the predation risk exposure period and tend to increase response over predation risk exposure time. Second, response to predator (visual cue) tend to peak at 48 hours cumulative exposure, but show dramatic decrease after 72 hours cumulative exposure. Finally, response to the mixed cue (Chemical+visual) tend to peak prior to the predation risk exposure period and show reduced response during subsequent exposure periods. This experiment confirms the lack of responsiveness to conspecific alarm signals in captive-bred M. rapidus and the need for an optimal nature behavior enhancement program prior to release of endangered species. Furthermore, responsiveness to predator visual signal peak at 48 hours cumulative exposure, suggest an optimal predation risk exposure period of up to 48 hours. Key words: predator cognition, captive breeding, chemical signal, visual signal, endangered

of hazardous risk factors, risk estimation and determination steps by reflecting the trend of overseas risk assessment. METHODS : In deriving, estimating and determining risk factors, comparing the procedures presented by the ILO with the domestic guidline to find out the differences in procedural. and, According to the domestic manual, after setting the criteria for determining a deterministic perspective, analyze the risk assessment data of a specific domestic company and three overseas risk assessment research data to analyze the differences in methodology domestic and abroad. RESULTS : Within the country, there is a possibility that a deterministic view may be applied to all stages of procedure, and certain corporate data to the risk estimation and determination stage. In the case of overseas, the trend of applying deterministic perspectives to the risk determination stage was confirmed. CONCLUSIONS : Present the need for a standard model for improving deterministic methods in the other two stages, excluding risk determination in the domestic evaluation procedure.

Herbicide-resistant transgenic rapeseed (TG rapeseed) was developed by inserting phosphinothricin acetyltransferase (PAT, bar), a modified gene from the soil bacterium Streptomyces hygroscopicus, into the genome of a conventional variety of rapeseed (Youngsan). The TG rapeseed used for the test was confirmed to express the PAT gene by polymerase chain reaction (PCR) and immunostrip. Feeding tests were conducted with Cyprinus carpio to evaluate the environmental risk of TG rapeseed, including the herbicide resistant gene. C. carpio was fed 100% ground rape suspension, TG rapeseed, or non-genetically modified (GM) counterpart rapeseed (Youngsan). As a result, the feeding test showed no significant differences in the cumulative immobility or abnormal response between C. carpio samples fed on TG rapeseed and non-GM counterpart rapeseed. The 48 h-LC50 values of the TG rapeseed and the non-GM counterpart rapeseed were 3,376 mg/L (95 % confidence limits: 3,169 - 3,596 mg/L) and 2,682 mg/L (95 % confidence limits: 2,267 – 3,123 mg/L), respectively. The rape NOEC (no observed effect concentration) value for C. carpio was suggested to be 625 mg/L. Based on these results, there was no significant difference in the toxicity for non-target organisms (C. carpio) between the TG rapeseed and non- GM counterparts.

This study analyzed the factors affecting the effectiveness of the Chemical Hazard Risk Management (CHARM). A survey was conducted on 104 learners who participated in the Risk Assessment training course at Occupational Safety and Health Training Institute. Through a self-administered questionnaire, the effect of personal characteristics, corporate characteristics, and safety and health level of the company on the effectiveness of chemical risk assessment was investigated. As a result of statistical analysis, the safety and health level of the company had a positive (+) effect on the effectiveness of Chemical Hazard Risk Management(CHARM), but personal characteristics and corporate characteristics had no relation to it. This study can be used as basic data for further research related to chemical risk assessment in workplaces.

Purpose: The purpose of this study is to understand the nature of the health promotion behavior of the high-risk group of cardiovascular disease among large-scale industrial manufacturing workers. Method: The subjects of this study were 11 workers at high-risk of cardiovascular disease with a Framingham Risk Score (FRS) score of 10% or more among workers at large-scale workplace in Gyeongsangbukdo area, and the data collection period is from July 1 to September 11, 2022. The interview data were inductively analyzed using the qualitative content analysis method used by Elo and Kyngas (2008). Result: Participants' awareness of their own health status and knowledge of cardiovascular disease were low, and there were more obstacles than benefits to health behavior. The process and method of realizing and practicing health care were also different. As a result of qualitative content analysis, 42 semantic units, 12 subcategories, and 3 upper categories were derived. The health promotion behavior of workers at high-risk for cardiovascular disease was categorized into ‘Awareness of health conditions’, ‘Obstacles to health care behavior’, ‘Health care practice process’. Conclusion: Since most of the workers spend a lot of time at work, it is necessary to understand the health care of high-risk workers with cardiovascular disease, so a qualitative study using participatory observation methods to observe workers’ work sites is recommended.

In this study, the AHP (analytic hierarchy process) technique was used to analyze the risk of expected risk factors and fishing possibilities during gillnet fishing within the floating offshore wind farms (floating OWF). For this purpose, the risks that may occur during gillnet fishing within the floating offshore wind farms were defined as collisions, entanglements, and snags. In addition, the risk factors that cause these risks were classified into three upper risk factors and ten sub risk factors, and the three alternatives to gillnet fishing available within the floating OWF were classified and a hierarchy was established. Lastly, a survey was conducted targeting fisheries and marine experts and the response results were analyzed. As a result of the analysis, among the top risk factors, the risk was the greatest when laying fishing gear. The risk of the sub factors for each upper risk was found to be the highest at the berthing (mooring), the final hauling of fishing net, and the laying of the bottom layer net. Based on the alternatives, the average of the integrated risk rankings showed that allowing full navigation/fisheries had the highest risk. As a result of the final ranking analysis of the integrated risk, the overall ranking of allowing navigation/fisheries in areas where bottom layer nets were laid was ranked the first when moving vessels within the floating OWF was analyzed as the lowest integrated risk ranking of the 30th at the ban on navigation/fisheries. Through this, navigation was analyzed to be possible while it was analyzed that the possibility of gillnet fishing within the floating OWF was not high.