Mongolia is known as the livestock country for 800 years from the period of Chinggis Khaan Empire. The livestock has been raised without corn, a high quality feed. Hence, the meat of Mongolia has not been exported. In 2004, International Corn Foundation (ICF) and KNU in Korea initiated corn breeding program for Mongolia with Mongolian Academy of Science and Agricultural University of Mongolia. Since that time approximately 2000 genotypes were planted in Agricultural Research Station in Darkhan, Mongolia. Within 4years of research we have succeeded in forming Mongolian corn population (MCP), which can be used to breed locally adapted varieties in different zones. MCP characterizes early maturity, cold and drought tolerances. In 2008, 160 S1 were planted and 16 lines were selected. For further inbreeding and population improvement we planted all 16 lines in KNU vinyl house at Kunwi in late September. F1 crosses and advanced S3 lines will be tested in 2009 first time for hybrid development in Mongolia.

Plant proteomic study requires a high-throughput separation to the detection and analysis of peptides and proteins by mass spectrometry (MS) to detect low abundant proteins. Together, efficient separation and MS can lead to the detection of thousands of plant proteins in a cell or tissue and help build proteome maps that can provide a global snap-shot of the cell or tissue status. Recently efficient separations based on the HPLC were introduced to allow deeper protein detection and improve throughput. For the HPLC based methods, Multidimensional Protein Identification Technology (MudPIT) and 1D-Gel-LC-MS/MS will be introduced. In MudPIT analysis, all proteins in a sample are digested into peptides before the separation step then the mixture of peptides are separated through the biphasic capillary column and sequentially eluted into the mass spectrometer and analyzed. 1D-Gel-LC-MS/MS separates protein samples in 1D-SDS-Gel then the proteins in each band were ingel-digested into peptides followed by peptides separation with Reverse Phase column and elution into the mass spectrometer. The main goal of this presentation is to introduce the recent protein separation and identification methods based on the HPLC coupled with MS analysis including conventional method of 2D-PAGE.

인삼 연작장해 원인과 경감대책을 수립하고자 2004년에는 2005년까지 작물과학원 증평시험지 연작토양에서 인삼 수확경 과년수와 예정지관리 유무에 따른 시험을 수행한 결과를 요약하면 다음과 같다. 인삼 연작지 수확경과년수가 짧을수록 생존율이 낮아지며 3~4년 경과구 81~95%의 뿌리썩음 이병주율을 보였다. 예정지 관리구는 미관리구보다 생존율이 다소 높으며 뿌리 썩음병 피해도 예정지 미관리와 같은 경향으로 예정지 관리효과는 크지 않다. 수확경과년수가 10년이상의 연작지에서도 연작장해를 일으키는 병원균 (C. destructans)이 분리되므로 재작 또는 연작시는 이점을 고려하여 재배지를 재선정하거나 예정지 토양관리대책을 수립해야 할 것으로 판단된다.

Pig organ is thought to be the most suitable nonhuman organ for xenotransplanstation. However, one of the major constraints to using pig organs for xenotransplantation is human natural antibody-mediated hyperacute rejection (HAR). Elimination of a(1,3) galactosyltransferase (GGTA1) from the pig is expected to be a solution to the problem of hyperacute rejection. Many efforts have made characterization of GGTA1 in structure and function, improvement in the technique of DNA transfection of somatic cells and advancement of the pig NT, a specific modification has been made to one copy of the GGTAl gene by Missouri group in 2002 To date because homozygousity of the genetic modification has been achieved in this gene, the role of gala(1,3) gal specific natural antibody in HAR and the efficacy of xenotransplantation in a nonhuman primate model will be addressed. Of other genes are found to be involved in rejection of pig donors by primates, the technology will be available to modify those genes so that rejection can be overcome.

This study wi1l examine the Eliot‘s reaction to the American Puritanism. One of the remarkable characteristics of American Puritan society was that it kept its balance between two contradictory doctrines. While the American Puritans had the Calvinistic notion of original sin, they emphasized their self-confidence and pride as the chosen people and believed that they had been already saved as New Israelites of the City of God. As a result, they paradoxical1y came to dilute the doctrine of original sin. It is the American Puritan jeremiad that reveals this paradox and has worked through American rhetoric as the ideology accelerating Americanization up to the present. Though it was a kind of reprimand and lamentation, the jeremiad was at the same time the rhetoric that directed an imperiled people of God to fulfill their destiny and guided them individually toward salvation and collectively toward the American City of God. This study examines Eliot’s reaction against such an optimistic progressive rhetoric. In a sense, E1iot as a Christian poet should be fundamentaIIy optimistic. Therefore it might be said that he is opposed not to optimism or progress itself, but to shallow such optimism and blind belief in progress without understanding of human life as Eliot thinks is Emerson’s Transcendentalism. And it is the transition from American Puritanism to Transcendentalism that Eliot pays attentlOn to. In the course of his reaction to American Puritanism inc1uding Unitarianism and Transcendentalism as a sequence of American Puritanism, Eliot in turn criticizes humanism for the be1ief in the goodness of human nature and the ignorance of original sin, which drives the modem world to what he regards as wrong, i. e., Romanticism, Democracy, and Protestantism. Therefore what concems Eliot the most about the modem world is the disappearance of the sense of sin, which, he argues, is another product of the American Puritan optimism.

Now, many anticancer drugs are applying in the clinical side, but there is no conclusive effect of such a chemotherapy. Development ofnovel clinical useful anticancer drugs would be dependenton the screen-ing system and its test sample sources. So, it is necessary to outlinesome background on the tumor systems which have been used for screen-ing. This paper describes mainely on National Cancer Institute (NCI)program for anticancer screening systems, because the large number ofcompounds have been screened at NCI prograB and their relationship ofassesment between experimental animals and clinical Patients has beendiscussing and the uniform screefing protocols for various tumorsystens. At the end of this paper, some literatures of antitunor substances from various higher Plants at our laboratory are showed.