This study was conducted to examine the effects of human follicular fluid and gonadotropin (FSH+HCG+rhEGF) on in vitro maturation, fertilization and development of human immature oocytes. Cumulus-oocyte complexes (COCs) were collected following for in vitro fertilization and embryo transfer (IVF-ET) cycles of the patients. At the time of oocytes collection, oocytes were classified into MII, MI and GV in accordance with their appearance (MII: Fully mature oocyte at metaphase II of meiosis; MI: Nearly mature oocytes at metaphase I of meiosis; GV: Immature oocytes at prophase I of meiosis). After controlled ovarian stimulation using gonadotropin(FSH) and human chorionic gonadotropin (HCG) in 70 ICSI cycles, 158 MI to MII matured oocytes were intracytoplasmic sperm injection (ICSI) h after in vitro culture and 553 MII oocytes were ICSI after denudation. The aspirated MI and GV oocytes were cultured in culture medium containing 10% (v/v) serum protein substitute (SPS), 10% (v/v) human follicular fluid (hFF) and 10% (v/v) serum protein substitute (SPS)+1 IU/ml FSH+10 IU/ml HCG+10 ng/ml recombinant human epidermal growth factor (rhEGF). The maturation rate of immature oocytes was similar among the three group. When maturation medium was supplemented with 10% SPS, 10% hFF or gonadotropins, the fertilization rate of in vitro matured oocytes was higher in 10% SPS (80.0%), but there was no statistical significance (78.2%; hFF, 76.9%; gonadotropin, p>0.05). The development rate of human embryos developed to cells were not significant difference in the medium containing SPS, hFF and gonadotropins (65.6%, 65.9% and 66.7%). The results of these study suggest that human follicular fluid and gonadotropins supplemented in the culture medium was not effected on the in vitro maturation, fertilization and development of human immature oocytes.

Since the computer technology has been drastically developed and broadly employed for the design of human machine system, human system interface was somehow digitalized. Actually the operator’'s working environment employing the digital devices are not be

Thepurposeofthisstudyistwo folds:one is to present different perspectives between Koreans and foreignersinconnectingaspecificwordintermsofassociatingantonyms; the other is to suggestsome effective adoptions ofthese findings in teachingKoreanvocabulariestoforeignlearnersofKorean.Itisoftenthe casethatpeopletendtothinkofaspecificwordtogetherwithitsrelated antonym.The relationship between a word and its antonym is closely associated with the vocabulary network in the human brain.Therefore using thisassociationinteachingKoreanhelpslearnerstorememberthe vocabularieseasier.Howeverthesemanticfieldofeachwordisinevitably different depending on the culturalbackground of the learners.This research reveals thatKoreans and foreigners often think ofa different antonym ofthesameword.Althoughtheassociationprocessseemstobe similarbetweenKoreansandforeigners,thereseemstobeadiscrepancy in theextentofabstraction ofantonyms.In otherwords,thedifference arisesnotinthetypeofantonyms,butintheextentoftheabstractionof eachword.Basedonthis,Koreanteachershavetobeespeciallycautious inteaching antonymsby dealing with gradableantonymsanddirectional antonyms in a differentmanner.Itshould be also noted thatteaching Korean vocabulariescan beaccomplishedmoreeffectively byrecognizing andrespectingdifferentculturalbackgroundsofthelearners.

당뇨망막병증은 서구에서 성인들의 실명을 일으키는 원인이다. 당뇨병이 있을 때 고혈당증은 여러 세포 형태에서 세포자연사를 유도하지만 그 기작은 명확하게 밝혀지지 않았다. 본 연구의 목적은 인간망막 내피세포에서 고혈당 포도당이 세포자연사에 미치는 영향에 대하여 알아보았다. 망막 내피세포는 5, 25, 50 mM 포도당이 포함된 IMDM배지에서 37℃, 5% CO2조정된 항온기에서 24, 36, 48시간 동안 배양하였다. 여러 농도의

Intracellular reactive oxygen species(ROS) produced in a various pathologic state was known to intermediate many cellular response such as inflammation. Recently, low level light irradiation by HeNe laser used in many clinical field could improve inflammatory state by scavenging intracellular ROS through photo-detachment/dissociation process. The purpose of this study is to investigate the differential effects of blue and red light irradiation on ROS scavenging effects. Immortalized human oral keratinocyte HaCat cells were used. Phorbol 12-myristate 13-acetate(PMA) was treated for inflammation. Red(635nm) and blue(470nm) light irradiation was carried out. To asses the intracellular ROS by light irradiation, confocal microscopic and flow cytometric assay with DCF fluorescence for total ROS and ESR spectrometry of DMPO-O2 - for superoxide anion were caried out. And microarray was performed for mRNA expression level. Released intracellular total ROS in PMA treated HaCat cell lines was dissociated efficiently by red light irradiation, while blue light irradiation did not. Rather, blue light irradiation increased ROS formation. For superoxide anion generated the first synthetic form of ROS, red light irradiation reduced its amount but blue light irradiation did not. In the mRNA expression in line with cyclooxygenase(COX) pathway, prostagrandin endoperoxide synthase 1(PTGS 1), prostagrandin endoperoxide synthase 2(PTGS 2) and phospholipase A2(PLA2) were increased by both light irradiation and they were decreased as time flows. And genes associated with ROS releasing, mRNA expressions of tumor necrosis factor receptor (TNFR) and interleukin 1beta(IL1B) were increased by 1 hour red light irradiation but did not by blue light irradiation. As a result, red and blue light irradiation showed different response in affecting the level of ROS. These findings indicate that red light rather than blue light is more useful for anti-inflammation in clinical field

The psychosocial stress and musculoskeletal disorders(MSDs) have been one of major health problems for hospital workers. This study tried to understand the relationship between symptoms associated with MSDs and risk factors such as working posture, job stress, psychosocial stress and fatigue. A total number of 655 hospital workers participated in this study. Specifically, REBA was applied for evaluating working posture and a checklist prepared by KOSHA(Korean Occupational Safety and Health Agency) was used for symptom survey. A questionnaire from KOSHA was also used for collecting data associated with job stress, psychosocial stress and fatigue. All these data were formulated and modeled by path analysis which was one of major statistical tools in this study. Specifically, path analysis for the data we collected came up with several major findings. The risk scores from working posture based on REBA had indirect effects via fatigue factor(MFS) as well as direct effects on symptoms. The factors associated with job stress (KOSS) and psychosocial stress(PWI-SF) had significant effects on symptoms. Specifically, indirect effect of job stress factors via fatigue factors(MFS) had bigger than that of direct effect of job stress on symptom.

The psychosocial stress and musculoskeletal disorders(MSDs) have been one of major health problems for hospital workers. This study tried to understand the relationship between symptoms associated with MSDs and risk factors such as working posture, job stress, psychosocial stress and fatigue. A total number of 655 hospital workers participated in this study. Specifically, REBA was applied for evaluating working posture and a checklist prepared by KOSHA(Korean Occupational Safety and Health Agency) was used for symptom survey. A questionnaire from KOSHA was also used for collecting data associated with job stress, psychosocial stress and fatigue. All these data were formulated and modeled by path analysis which was one of major statistical tools in this study. Specifically, path analysis for the data we collected came up with several major findings. The risk scores from working posture based on REBA had indirect effects via fatigue factor(MFS) as well as direct effects on symptoms. The factors associated with job stress(KOSS) and psychosocial stress(PWI-SF) had significant effects on symptoms. Specifically, indirect effect of job stress factors via fatigue factors(MFS) had bigger than that of direct effect of job stress on symptom.

본 연구의 목적은 인간의 간섭 및 영향에 따른 도시지역에서의 식물종의 분포패턴 및 특성을 분석하는데 있다. 전주시에서 자생적으로 이입된 모든 유관속 식물종에 대한 포괄적 자료습득을 위하여 각 1헥타 크기의 106개 표본 조사구가 분석되었다. 이러한 표본조사구는 전주시의 전형적인 토지이용유형을 대표하고 도심지역으로부터 다양한 이격거리를 갖도록 선정되었다. 종풍부성, 생활형의 패턴 그리고 외래종의 비율이 표본조사구의 헤메로비 정도와의 관계에 대해서 분석되었다. 식물종의 수는 적당히 영향을 받는 부지에서 가장 높게 나타남을 알 수 있었다. 이는 적당한 교란은 종풍부성 증가를 유도한다는 중간 교란 가설에 상응하는 결과이다. 인간의 영향을 가장 많이 받는 부지의 식물상은 일년생 식물과 외래종의 비율이 가장 높은 특징을 보였다. 그리고 일부 식물종들은 토지이용유형과 도시구역에 상응하는 유사한 분포를 갖는 세 가지 그룹인 도시지역 기피종(땅비싸리, 졸참나무, 개옻나무 등), 도시지역 및 외곽지역에 널리 분포하는 종(닭의장풀, 개망초, 박주가리 등)과 도시지역 선호종(애기땅빈대, 개쑥갓, 서양민들레 등)으로 구분되었다.

Development of human-system interfaces (HSI) supporting the interaction between human and automation-based systems, particularly safety-critical sociotechnial systems, entails a wide range of design and evaluation problems. To help HSI designers deal with these problems, many methodologies from traditional human-computer interaction, software engineering, and systems engineering have been applied; however, they have been proved inadequate to develop cognitively well engineered HSI. This paper takes a viewpoint that HSI development is itself a cognitive process consisting of various decision making and problem solving activities and then proposes a design requirements-driven process for developing HSI. High-level design problems and their corresponding design requirements for visual information display are explained to clarify the concept of design requirements. Lastly, conceptual design of software system to support the requirements-driven process and designers' knowledge management is described.

The purpose of this study was to compare the efficiency of slow freezing with that of vitrification method for the cryopreservation of human embryos. Human embryos were derived from in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and the mixed solution of propanedial (1.5, 1.0, 0.5M PROH) and sucrose (0.1M), ethylene glycol (7.5, 15%), dimethyl sulfoxide (7.5, 15% DMSO), sucrose (0.5, 1.0M) and SPS (Serum Protein Substitute) was used for a cryoprotectant for slow freezing and vitrification solution, respectively. Rates of recovery after thawing, morphological normality, post-thaw viability, arrest, morphological abnormality and preimplantation development were compared between two protocols. After freezing-thawing, recovery and survial rate of slow freezing was (88.6% and 73.4%), whereas vitrification was (99.2% and 96.2%) (p<0.05). The arrest rate of slow freezing was significantly lower compared with those of vitrification(8.7% vs 29.9%) (p<0.05). Preimplantation development to the 2-cell (83.8% vs 67.7%), 4-cell (69.0% vs 47.2%) and 8-cell (62.4% vs 37.8%) stages 24, 48 and 72 h after thawing, respectively, were higher in the slow freezing than the vitrification. After slow freezing and vitrification of human embryo at 2-8cell stage, the rate of recovery rate, survival rate and partial damage rate were 92.0% vs 100%, 80.4% vs 96.2% and 52.2% vs 19.0%, respectively. And partial damage rate was significantly lower than those of slow freezing method (p<0.05). These results demonstrate that a slow freezing using PROH is more efficient than a vitrification for cryopreserving the human zygotes, although the vitrification yielded better recovery, survival and partial damage of frozen-thawed 2-8 cell stage embryos than slow freezing method.

Humans are well-known for being adept at using intuition and expertise in many situations. However, human experts are still susceptible to errors in judgment or execution, and failure to recognize the limits of knowledge. This would happen especially in semi-structured situations, in multi-disciplinary settings, under time or other stress, under uncertainty, or when knowledge is outdated Human errors are caused by cognitive biases, attentional slips/memory lapses, cultural motivations, and missing knowledge. The purpose of this research is to study errors of human experts committed in judgment and the general idea of critiquing systems as corresponding plan. Compared to expert systems, critiquing systems are narrowly focused programs useful in limited situations for collaborating with and supporting experts in their task activities. It supports an expert by detecting the human's errors by deploying various strategies that stimulate humans to improve their performance. A variety of types of critiquing systems has spread through numerous application areas.