표고 재배용 액체종균 배지조성은 대두박분 배지에 입도가 850 μm이하인 참나무분 0.3% 첨가 시 균사체 건중량이 457 mg으로 다소 높았으며, Laccase 활성도는 참나무분 첨가율별 대두박분 배지에 큰 차이가 없었으나 Cellulase 활성도는 CMC Agar Plate에서 26.4 mm로서 참나무분 첨가율별 대두박분 액체종균 처리구중에서 가장 우수하였다. 액체종균의 통기량이 증가할수록 이산화탄소 발생량이 증가하여 배양 7일째 최대치를 나타내었으며 균사체 건중량은 10일째 3.0g/l로 가장 우수한 것으로 나타났다. 통기량이 증가할수록 이산화탄소 발생량, 균사체 건중량도 증가하였으며 0.6vvm 처리시 배지 증발율이 32%, 균사체 건중량이 3.5g/l로서 우수하였다. 선발된 액체종균을 톱밥 종균과 생산성 비교를 위하여 톱밥재배 봉지배지에 시험한 결과, 액체종균의 생육기간이 톱밥종균에 비해 1일 지연되었으나, 봉지당 유효 개체수와 수량에는 유의적인 차이가 없는 것으로 나타났다.

Synthetic wood has been widely used in place of wood such as the building exterior materials and/or the floor plate of moving path. However, it has many disadvantages such as relatively low strength, low durability, and heavy weight, etc. In this study, the synthetic wood deck combined with an aluminum profile was suggested to overcome these disadvantages. The flexural strength of the aluminum-synthetic wood deck was obtained through the theoretical equations under three-point bending conditions. In addition, the finite element analyses were also conducted to evaluate the flexural strength of the aluminum-synthetic wood deck.

One of engineered woods, glued laminated timber (GLT), can provide a constant level of performance and desired strength even if the quality of wood is low. Due to this fact, there is a growing interest in GLT using domestic species and related research has been carried out continuously. In addition, GLT is popularly being applied to the long-span or high-rise structures overseas. However, KBC 2016 does not allow the engineered woods to be used for middle and high-rise buildings by limiting height. Therefore, a proper design procedure and rationale should be clearly presented by the help of performance-based seismic design. With this background, the goal of this study is to establish a specific procedure for design of a 9-story building with RC shear walls and GLT frames according to the performance-based design of KBC 2016. The performance objectives were set according to KBC and the acceptance criteria for each goal were defined. The RC shear walls and GLT frames were designed by concrete and wood structure requirements, respectively. Analytical models were developed to reflect their nonlinear features, and both nonlinear static and dynamic analyses were conducted. Performance evaluation results showed that the shear walls have insufficient shear strength, so they were re-designed. Consequently, it has been confirmed that GLT frames can be applied to a 9-story office building with the assistance of RC shear walls and performance-based seismic design.

Structural insulated panels, structurally performed panels consisting of a plastic insulation bonded between two structural panel facings, are one of emerging products with a viewpoint of its energy and construction efficiencies. Of the SIPs, Cyclic test was conducted by two kinds of specimens: single panel and double panels. Cyclic test results, which were equivalent to static test results, showed that maximum load was 45.42kN, allowable shear load was 6.3kN/m. Furthermore the accumulated energy dissipation capability for double panels was as 2.3 times as that for single panel. From performance of structural tests, the allowable shear load for panels was suggested to be at least 6.1kN/m.

소나무재선충은 북미가 원산으로 일본, 중국, 한국을 비롯한 동북아시아의 소나무류에 막대한 피해를 주고 있으며 포르투칼과 스페인에도 발생하여 유럽 소나무류에도 큰 위협이 되고 있다. 소나무재선충은 매개충인 Monochamus속에 의해 새로운 기주로 옮겨지기 때문에 매개충을 방제하는 것이 가장 중요하다. 국내 소나무재선충 매개충은 솔수염하늘 소와 북방수염하늘소가 보고되어 있으며 이들을 방제하기 위하여 주로 유기합성농약을 항공 살포하거나 연막기를 이용하여 살포하고 있다. 하지만 유기합성농약의 대면적 살포는 산림생태계에 큰 위협요인이 되고 있다. 본 발표에서는 유기합성농약을 대체하기 위하여 소나무재선충 매개충의 집합-성페로몬 및 카이로몬 연구 그리고 이들 유인제의 현장적용 연구 및 추후 연구방향에 대해 소개를 하고자 한다.

Pine wood nematode (PWN), Bursaphelenchus xylophilus causes devastating damage to pines worldwide. To develop new agent for controlling PWN, alkyloxyalcohols(A-H) were synthesized and nematicidal activity was tested against PWN. As a control, monochamol, which reported to have nematicidal activity was also tested. The nematicidal activity was diffferent according to carbon chain length in compounds. LC50 values of F, G and H were as same as that of monochamol. However A-E which have shorter carbon length than F-H showed weak nematicidal activity.

본 연구에서는 수입되는 바이오매스를 대체하고 증가하는 국내 RPS의무비율을 보다 효과적으로 대응하기 위해 우드펠릿으로 사용가능한 국내 산림바이오매스 부존자원을 파악하기 위하여 선행연구 방법과 매년 추가로 성장하는 임목생장률을 기준으로 미이용 산림바이오매스의 양을 산정하였다. 그 결과, 임목가공 중 발생하는 부산물 중 20%를 우드펠릿 원료로 사용한다고 가정했을 경우 두 가지 추정 방법으로 도출된 평균값을 기준으로 우드펠릿 생산 가능량을 예측 하였다. 그 결과 미이용 부산물은 2016년 199만 톤, 2020년 228만 톤, 2030년 308만 톤이 발생되고 원목가공 과정에서 발생되는 임목부 산물(피죽, 톱밥 등) 중 20%가 우드펠릿 원료로 활용될 경우 2016년 258만 톤/년, 2020년 295만 톤/ 년 2030년 398만 톤/년의 원재료가 추가되어 미이용 부산물과 원목가공 과정 중 발생되는 부산물로 생산 가능한 우드펠릿 양은 2016년에 274만 톤/년, 2020년 314만 톤/년 2030년 423만 톤/년의 우드펠릿이 생산 가능하다는 결과를 도출 하였다.

소나무재선충(pine wood nematode, PWN) 감염 소나무를 현장에서 신속하게 진단을 할 수 있는 방법은 현재 없다. 본 연구에서는 PWN특이적인 항원으로 알려진 PWN-GaLectin을 baculovirus발현체계로 발현시켜서 총 1,464개의 fusion hybridoma 세포 주 라이브러리 를 제작 하는 항원으로 이용하였다. 총 1,464개의 fusion hybridoma 세포 주 중, PWN-GaLectin에 대한 높은 반응을 보이는 62개의 fusion hybridoma 세포 주를 선별했다. 이들 중, 표준 PWN 감염소나무 PBS추출물과 PWN 단백질 추출물에 강한 반응을 보이는 세포 주 12개를 선 별하여 단클론 항체(monoclonal antibody, Mab) 분비세포 주 확립을 위한 limited dilution을 실시하였다. Mab분비세포 주 확립을 위해서 표 준 PWN 감염소나무 추출물에 대한 반응이 표준 정상 소나무 추출물 보다 높은 세포 주들을 선별했다. 그리고 추가로 PWN 단백질 추출물에 대 해서 3종의 비 병원성 선충 단백질 추출물 보다 높은 반응을 보이는 세포 주들도 선별, 확립했다. 본 연구에서 확립된 Mab들을 우리는 현장과 실 험실에서 사용할 수 있는 신속진단키트의 개발에 이용할 수 있을 것이다.

신·재생에너지 보급통계에 의하면 바이오매스 발전실적은 2013년 부터 급증하고 있으며 그 중에서 가장 급격하게 증가한 연료는 Wood pellet으로 2013년 696Gwh, 2014년 2,764Gwh, 2015년에는 2,512Gwh를 발전 하였고 국내 Wood pellet 총 소비량은 2015년 기준 148만톤이며 그 중 발전용으로 소비된 Wood pellet은 108만톤으로 약 73%를 차지하고 있다. 본 연구에서 Wood pellet 소요량을 예측한 결과 국내 발전용으로 필요한 Wood pellet 소요량은 2020년 261만톤, 2025년 685만톤, 2030년 1,139만톤이 필요하며, 최적 바이오매스 발전량 산정을 위하여 바이오매스 발전소에서 국내 생산 Wood pellet 사용량을 50% 사용한다는 가정하에 기허가 신청된 발전소를 가동하기 위해서는 2021년 226만 톤의 Wood pellet이 국내에서 생산되어야 한다는 결론이 도출되었다.

To evaluate effects of ligninolytic enzyme type on the mycelial response and ligninolytic enzyme production during interspecific interactions among wood-rotting fungi, 4 fungal strains, Trichophyton rubrum LKY-7, Trichophyton rubrum LSK-27, Pycnoporus cinnabarinus, and Trichoderma viride, were selected. Regarding ligninolytic enzyme production, LKY-7 secreted laccase and manganese peroxidase (MnP), P. cinnabarinus secreted only laccase, and LSK-27 secreted only MnP in glucosepeptone medium, while T. viride did not produce any ligninolytic enzymes. In the co-culture of LKY-7 with P. cinnabarinus, the formation of aerial mycelium was observed and the enhancement of laccase activity owing to interspecific interaction appeared to be very low. In the co-culture of LKY-7 and P. cinnabarinus with LSK-27, a hypha-free clear zone was observed, which resulted in deadlock, and increased laccase or MnP activity was detected at the interaction zone. The interaction responses of LKY-7, P. cinnabarinus, and LSK-27 with T. viride were characterized by the formation of mycelial barrages along the interface. As mycelial barrages were observed at the T. viride territory and no brownish pigment was observed in the mycelial barrages, it is suggested that laccase and MnP are released as part of an offensive response, not as a defensive response. The co-culture of P. cinnabarinus with T. viride lead to the highest enhancement in laccase activity, yielding more than 14-fold increase in laccase activity with respect to the mono-culture of P. cinnabarinus. MnP activities secreted by LKY-7 or LSK-27 was generally low in interspecific interactions.

Wood pellets are used by Biomass-solid refuse fuel (Bio-SRF) has been imported from many East-Asia countries especially,Vietnam in Korea. Wood pellets are made through grind and high-pressure process shows different physical characterizationlike high methyl bromide (MB) absorption unlike other wood materials. Furthermore, detected pests in wood pellets havesimilarity with detected pests in stored grain. In this study, we analyzed susceptibility on pupa and adult stages of redflour beetle, Tribolium castaneum in wood pellets against mixture of MB and phosphine (PH3). Mixture treatment ofMB and PH3 was more effective than single MB treatment compare with control. Pupal stage showed more tolerant thanadult stage on each fumigant and 100% mortality was determined at 27 mg/L of MB only at 20°C. However, PH3 increasedMB toxicity on pupae that 100% mortality was observed at 100:1 ratio of MB (5 mg/L) and PH3 (0.05 mg/L) at 20°C.Based on this study, mixture treatment of different type of fumigant will be applied to control of quarantine pests.

In this study, we isolated and identified an aggregation-sex pheromone from Monochamus saltuarius, the major insectvector of the pine wood nematode in Korea. Adult male of M. saltuarius produces 2-undecyloxy-1-ethanol, which is knownto be an aggregation-sex pheromone in other Monochamus species. We performed field experiments to determine the attractivenessof the pheromone and other synergists. More M. saltuarius adult beetles were attracted to traps baited with the pheromonethan to unbaited traps. Ethanol and (-)-α-pinene interacted synergistically with the pheromone. Traps baited with pheromone+(-)-α-pinene+ethanol were more attractive to M. saltuarius adults than traps baited with pheromone, (-)-α-pinene, or ethanol alone.Ipsenol, ipsdienol, and limonene were also identified as synergists of the aggregation-sex pheromone for M. saltuarius adults.

We evaluated the larvicidal and nematicidal activities of 48 3-acylbarbituric acids analogues against the Asian tigermosquito, Aedes albopictus and the pine wood nematode, Bursaphelenchus xylophilus, organisms of increasing global concern.Among 48 3-acylbarbituric acids analogues, four compounds 10, 14d, 14g, and 19b showed >90% larvicidal activity againstAe. albopictus at 10μg/mL concentration, and one (compound 10) showed the strongest larvicidal activity against Ae. albopictus,with a LC50 value of 0.22μg/mL. Only compound 18 showed strong nematicidal activity against pine wood nematode.Most active compounds possessed similar physicochemical properties; thus, actives typically had ClogP values of around1.40-1.50 and rel-PSA values of 16-17%.

The purpose of this study was to comprehend the assignment and the procurement method of wood works’ craftsman through the Salleung-dogamuigwes in royal tombs constructions in the late Joseon period. Following conclusions have been reached through the study. First, Carpenter and other nine craftsmen were introduced in carpentry works. The craftsmen were put in by occupation to maintain the quality of the government building construction by wood processing area. It was distinguished by frame member, Sujangjae, window framing wood, decorative member. Second, sawyer showed a sharp increase in the latter half of the 18th century and declined since the middle of the 19th century. This is closely related to the increase in demand for building materials and the growth of civilian wood products. As a maximum of 300 people have been assigned to the craftsmen in the woodwork, it can be seen that the work has been divided by the process. Third, the national carpenter was difficult to procure from the 17th century to the beginning of the 18th century. From the middle 18th century, the system procuring national carpenter was stabilized. In the 19th century, the input ratio of the national craftsmen seems to have decreased significantly and seems to have kept it at the minimum level. Forth, sawyer were procured through Seongonggam from the late 18th century through the mid 19th century. That means that the role of the sawyer had become important. Since Jogakjang is not a necessary manpower, it usually has civilian artisans.

The need for eco-friendly building materials such as engineered wood has increased to reduce carbon emissions. Although the range and height of engineered wood buildings are gradually increasing in North America and Europe, engineered wood is mainly used for low-rise residential buildings in Korea. In order to reduce carbon emissions more, therefore, it needs to expand the use of engineered wood by applying it to various buildings with different uses or more stories. With this background, the aim of this study is to investigate the applicability of engineered wood for 9-story office buildings. Since a 9-story building with engineered wood only is not allowed in KBC, an example building has RC ordinary shear walls as the lateral force resisting system while engineered wood is only used for gravity load resisting moment frames. Another example building is also used for comparison where both lateral and gravity load resisting systems are designed by RC. The applicability of engineered wood is investigated by comparing the seismic performance and the amount of carbon emission of both buildings. The result shows that the seismic performance of both buildings was not significantly different while the amount of carbon emission of the engineered wood building was much less then the RC building. Based on this result, engineered wood is sufficiently applicable to 9-story office buildings even though it still needs to pay attention to the shear design of reinforce concrete walls.

Pine wilt disease is currently the most deadly forest disease in the world and is known to be caused by infection of Bursaphelenchus xylophilus. Until now, no method has been developed to confirm the pine tres infected with pine wood nematodes. In order to develop a method to diagnose pine wood nematode infection, we produced a monoclonal antibody against Expansin B3, which was found to be secreted by pine wood nematodes. ELISA assay using various monoclonal antibody confirmed that the pine trees infected with the rewarming can be detected. Further studies using our antibodies may help to develop a diagnostic method that can quickly confirm infection of pine wood nematodes in the field.

Monochamus alternatus (M. alternatus) and Monochamus saltuarius (M. saltuarius) are major vectors for Bursaphelenchus xylophilus in South Korea. When an adult, they are easily distinguishable by several morphological classification. However, it is difficult to identification between M. alternatus and M. saltuarius when they are larvae as they have very similar morphological characters. Thus, they are not easily distinguishable without expertise about Cerambycidae taxonomy. Furthermore, during epidemiological investigation, sometimes, adults or larvae would not be founded in death pine trees. For these reasons, in this experiment, we are able to identified between M. alternatus and M. saltuarius by mitochondrial 12S rRNA gene primers that are specific to 12S rRNA gene fragment of M. alternatus using larvae tissue and frass. Moreover, we had examined whether vectors that were already escaped from dead pine tree have Bursaphelenchus xylophilus or not by multiplex PCR using larva frass that was remained in dead pine tree.

A biofilter filled with sintered glass media and wood bark media were developed and tested. Acetic acid and ammonia added in brewery wastewater were used as an artificial odor source. The Reynolds’ number (NRe) was below 130 in the loading range of 3~5 m3/m2-min, while the pressure drop was less than 6 mmH2O. The average removal efficiency of acetic acid was 87.6% and 71.5% at surface loading rate of 3.1 m3/m2-min and 4.4 m3/m2- min, respectively. The acetic removal capacities were 8.1~14.3 g/m3-min with the mass loading rates of 11.7~22.4 g/m3-min, indicating very high performance. However, the acetic removal capacity was sharply decreased at the mass loading rate of 20 g/m3-min. The average removal rate of ammonia was 38% and 25% at the surface loading rates of 3.1 m3/m2-min and 4.4 m3/m2-min, respectively. The ammonia removal capacity was 0.47~0.88 g/m3-min in the range of 11.7~22.4 g/m3-min mass loading rates. The intensity of complex odor was also decreased based on the findings in the measurement using the direct olfactory method and the GC analysis.