This study conducted to analyze the growth characteristics of old Prunus yedoensis on Jeju island. The diameter growth per year was measured using a DTRS-2000 instrument. The DBH, ground DBH and height of the investigated P. yedoensis were 137 cm, 143 cm, and 15.5 m, respectively. Our analysis showed that the age of the old P. yedoensis was 93 years. An annual diameter growth of 2.85 mm±0.96 was observed. The result of age estimation, about 265±64 years in P. yedoensis on Jeju island. This information could be useful to understand the annual diameter growth characteristics the P. yedoensis distributed on Jeju island.

Carbonaceous material prepared from oriental cherry can be used for the adsorption of zinc ion from an aqueous solution. Parameters such as pH (4-11), temperature (293-333 K), mixing intensity (10-120 rpm) and contact time (0.5- 90 min) were studied. Increasing pH (99.6% at pH 11) and temperature (99.8% at 333 K) caused an increase in adsorption capacity. A pseudo-equilibrium state was reached within 1 min of contact time. Removal efficiency of zinc ion remained constant regardless of mixing intensity. The adsorption equilibrium data were best represented by the Freundlich adsorption isotherm. The calculated maximum adsorption capacity was 3.541 mg/g. Thermodynamic studies demonstrated that the adsorption process was spontaneous with Gibb’s free-energy values ranging between -3.272 and -15.594 kJ/mol and endothermic with an enthalpy value of 86.984 kJ/mol. Therefore, carbonaceous material from oriental cherry was shown to have good potential for the adsorption of zinc ion.

Background: In this study, we investigated the antibacterial and nitric oxide (NO) production inhibitory activities of 75% ethanol extract of Prunus sargentii branches and its fractions against acne pathogens. Methods and Results: The antibacterial activity against acne causing pathogens was determined using the disc diffusion assay. The ethyl acetate fraction showed higher activities against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis than those shown by other fractions. In the DPPH radical and NO scavenging assays, the butanol fraction showed strong DPPH radical and NO scavenging abilities. These activities were related to the total polyphenol and flavonoid contents of butanol fraction. On the other hand, the chloroform and ethyl acetate fractions exhibited the highest NO production inhibitory activity in Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells compared to those exhibited by other fractions. Conclusions: The extract and its ethyl acetate fraction from the branches of P. sargentii exhibited antibacterial activity and could be used as functional materials in antimicrobial related fields. Moreover, the chloroform and ethyl acetate fractions are potential antiinflammatory agents and butanol fraction acts as an effective radical scavenger.

This study was carried out to investigate the antibacterial activity against pathogens of acne and the anti-inflammatory effect of 75% ethanol extract and its fractions from the leaves of Prunus sargentii. In the antibacterial activity by the disc diffusion assay, the extract showed the highest effect against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis in 5 mg/disc. However, the ethyl acetate fraction showed the highest antibacterial activity in 1 mg/disc. On the other hand, the hexane and chloroform fraction showed strong nitric oxide (NO) production inhibitory effect in lipopolysaccharide (LPS)-stimulated Raw 264.7 cell. In the cell viability of Raw 264.7 by MTT assay, the extract and all fractions were exhibited normal viabilities as nontoxic result. Consequently, the extract from the leaves of P. sargentii and its ethyl acetate fraction could be applicable to functional materials for antibacterial activity related fields. Moreover, the hexane and chloroform fraction could be applicable to candidate materials as anti-inflammatory agent.

In this study, we investigated the antibacterial activity, antioxidative activity and whitening effect of 75% ethanol extracts from different parts of Prunus sargentii. The total phenolic compound content of the branch extract was 277.92 mg/g as the highest level. In the measurement of DPPH radical scavenging ability, SC50 values of the cork layer and branch extract were 26.79 μg/ml and 30.13 μg/ml. In nitric oxide (NO) scavenging ability, SC50 values of the branch and leaf extract were 49.19 μg/ml and 55.55 μg/ml. All extracts exhibited higher NO scavenging ability than ascorbic acid used as positive control. On the other hand, in antibacterial activity against Staphylococcus epidermidis and Staphylococcus aureus by disc diffusion assay, the pure bark extract showed the highest activity. Moreover, tyrosinase inhibitory activity of cork layer, pure bark and branch extracts showed higher activity than arbutin used as positive control. In the cytotoxicity measurement by MTT assay, leaf extract was exhibited Raw 264.7 cell viabilities of 44.68~61.83% as cytotoxic result in tested concentration. In conclusion, the branch extract of Prunus sargentii will be a functional materials without damage compared to other parts such as pure bark or cork layer in the plant.

Prunus sargentii of Rosaceae familiy, has been reported to have radical scavenging activity and anti-inflammatory effect. On these facts, this research was conducted to evaluate pharmaceutical activities of the bark extracts P. sargentii. Free radical scavenging activities of fraction (Fr) -5~10 isolated from P. sargentii was higher than 80% respectively at 10ppm. The superoxide dismutase (SOD)-like activity of Fr-5, 9 were about 97, 84%, respectively at 1,000 ppm. Xanthine oxidase inhibitory effect of Fr-9, 10 were about 75, 78%, respectively at 1,000 ppm. The tyrosinase inhibitory effect related to skin-whitening was 72, 68% in Fr-2, 9 isolated from Prunus sargentii R. at 1,000 ppm. Hyaluronidase inhibition activity related to the anti-inflammation effect was 98% for Fr-8 at 500 ppm. Isolation of the methanol soluble fraction from P. sargentii yielded two major phenol compounds, (-)-epicatechin and taxifolin. The structure of the compound was certainly determined by chemical analyses, as well as NMR and Mass spectroscopy. The present study was carried out in a search for new cosmetic material from the bark from P. sargentii. and, (-)-epicatechin and taxifolin were isolated as active principles. So P. sargentii R. methanolic extracts may be used for the cosmetic material.

벚나무 꽃과 유엽을 이용한 차개발을 위해 꽃과 유엽의 주요 성분분석, 항균성, 항산화성, 항염증 및 제다 방법에 따른 색도, 무기성분 변화 조사와 품질의 관능 평가 등을 실시하였다. 벚나무 꽃차를 80℃ 물에 우려낸 결과 K, Ca, Mg는 동결건조로 제다한 차에서, Na은 자연건조로 제다한 차에서 많이 추출되었다. 벚나무 꽃차를 우려내는 시간은 무기물의 추출 측면에서 5분 이상 우려내야 할 것으로 나타났다. 덖음 처리로 제다된 유엽차의 무기물을 효과적으로 용출하려면 덖음처리 단계에서부터 90초 정도의 덖음처리를 하는 것이 좋은 것으로 나타났다. 벚나무의 꽃차를 관능평가를 한 결과 꽃차의 색도와 향은 낮게 평가된데 비해 수색은 높게 평가되었다. 유엽차는 덖음처리를 한 것이 높게 평가된 가운데 색도는 다소 낮게 평가되었다. 그러나 향기는 구수하다라는 평가가 많았고, 수색은 녹차와 같다는 평가가 많았다.

벚나무 꽃과 유엽을 이용한 차 개발의 일환으로 꽃과 유엽의 일반성분 및 화학 성분을 분석하였다. 일반성분은 꽃의 경우 조단백질(6.39%), 조회분(2.28%), 조지방(2.01%) 순이었으며, 유엽은 조단백질(11.04%), 조회분(3.98%), 조지방(1.91%) 순이었다. 무기성분 함량은 꽃의 경우 칼륨(85.28cmol․kg-1), 칼슘(60.40cmol․kg-1), 인산(11.12cmol․kg-1), 나트륨(4.21cmol․kg-1)순이었으며, 유엽은 칼륨(122.79cmol․kg-1), 칼슘(63.31cmol․kg-1), 인산(11.52cmol․kg-1), 나트륨(4.86cmol․kg-1) 순이었다. 비타민 C는 꽃에서 0.85g/100g, 유엽에서는 0.95g/100g이 검출되었다. 구성당중 manitol은 꽃에 1.32mg/g, 유엽에 0.64mg/g이 함유된 것으로 나타났다. 아미노산은 18종류가 분리되었는데, 그 중 glutamic acid가 가장 많이 검출되었다. 지방산은 꽃이나 유엽 모두 linolenic acid가 각각 32.43, 65.15로 가장 많이 함유되어 있었다.

한라산에 자생하는 왕벚나무(Prunus yedonesis)의 미성숙 접합자배로부터 체세포배를 직접 유도할 수 있었으며, 이들 직접 체세포배로부터 식물체를 재분화 시킬 수 있었다. 0.1mg/L GA3 와 0.1mg/L BAP 또는 0.5mg/L GA3와 0.1mg/L BAP 가 첨가된 배지에서는 캘러스가 형성되지 않고 80~93%로 체세포배가 직접 발생하였으며, 그 중 정상적인 구형 또는 심장형의 체세포배 비율은 43~58%, 자엽상의 비정상적인 체세포배 비율은 42~57%로 나타났다. 자엽상의 비정상적인 체세포배는 생장조절물질이 첨가되지 않은 MS 배지에 계대배양하면 16주 이후부터 신초가 다수 발생되었고, 이들 신초들은 0.5mg/L IBA가 첨가된 MS 배지에서 80% 이상의 발근을 보여 정상적인 식물체로 발달하였다. 또한 종자 성숙 시기에 따라서 정상적인 체세포배는 만개 후 30일된 종자의 접합자배에서 전체 62.5%가 직접 발생되었으나 45일된 종자의 배에서는 37.5%가 발생되어 종자가 성숙할수록 체세포배의 직접적인 유도율은 점차 낮아졌다.