산형과 식물의 종자는 형태생리학적 휴면을 하고 있는 것으로 알려져 있다. 형태생리학적 휴면은 배가 미숙하고 생리적으로 휴면상태인 종자휴면중의 하나이다. 본 연구는 산형과의 세 종, 갯기름나물, 고본, 갯방풍 종자의 휴면타파와 발아에 미치는 온도, 광, 화학물질의 효과에 대하여 조사하였으며 그 결 과는 다음과 같다. 1. 본 시험에 사용된 종자의 크기는 길이 5.57 - 9.7 mm, 폭 3 - 7 mm 범위였고, 천립중은 갯기름나물 0.50 g, 고본 0.21 g, 갯방풍 17.53 g 이었다. 2. 예냉, 광, GA3, KNO3, 그리고 온도조건을 달리하여 전처리를 실시한 결과, 갯기름나물, 고본, 갯방풍의 최대 발아율은 각각 62.6%, 43.3%, 36.4%였다. 3. 갯기름나물은 4oC에서 7일 동안 예냉처리 하였을때 휴면 타파와 발아에 가장 효과적이었고 GA3의 저온 대체효과는 없었으며 20oC 항온조건에서 발아에 적합하였다. 4. 고본의 경우도 갯기름나물과 유사하게 4oC에서 7일간 예냉처리 하였을때 효과적이었으며 GA3와 KNO3 처리는 휴면타파에 효과가 없었다. 갯기름나물과 고본 두 종은 휴면타파와 발아를 위해 저온을 필요로 하였다. 5. 갯방풍은 GA3 200 mgL−1 처리가 발아에 효과적이었고 15/20oC(16/8h) 변온조건과 20oC 암조건에서 비교적 잘 발아하였다.

Background : Angelica tenuissima Nakai is a major medicinal plant around GyeoungBuk Area. Powdery mildew of Angelica tenuissima Nakai caused a Erysiphe heraclei DC. is a major disease. It was found leaf of Angelica tenuissima after rainy season. There was no agicultural chemical registration on Angelica tenuissima. Ecofriendly control needs Angelica tenuissima for GAP. Methods and Results : The seedlings used for the test were sowed and cultivated in mid-February at Bonghwa herbal crop research institute. The irrigation water was irrigated through a drip irrigation system. Powdery mildew occurred after rainfall lasted more than 2 days at the end of June. The pathogen (Erysiphe heraclei DC.) was identified by observing the diseased plants with an optical microscope. Bordeaux mixture was tested using Gold Bordeaux which was listed in the eco-friendly material list and the magnification was 500 times for the medicinal efficacy test and 250 times for the damage test. For the chemical treatment 40 ㎖ of Bordeaux mixture was added to 20 ℓ of water to prepare 500 times of the solution and the treatment was sufficiently wetted to the plant at about 8:00 am. The first treatment was treated at 7. 5 days, the second treatment at 7. 13 days, the third treatment at 7. 26 days, and the final investigation at 7.26 days. The occurrence of powdery mildew was sufficient to evaluate the treatment effect at 13.0% in untreated control and the control value was low at 33.5% in one treatment but 74.0% in control after 2 treatments showed significant control effect. 89.2% respectively. As a result it is effective to control the powdery mildew of Bordeaux solution at least once every seven days to improve the control effect. Conclusion : Control value of bordeaux mixture was higher than after two times of treatment. Bordeaux mixture treatment of powdery mildew on Angelica tenuissima control 89.2% and it can use a producing ecofriendly agricultural product.

Angelica tenuissima, also known as Ligusticum tenuissimum, is classified as a food-related plant and has been used as traditional medicines treating headache and anemia in Asia. However, its anti-melanogenic effect has not been reported in detail. When the extract of Angelica tenuissima (ATE) was prepared by the extraction with 70% EtOH at 80°C (final yield = 22%), the contents of decursin and Z-ligustilide in ATE were determined 0.06% and 8.43%, respectively. Total flavonoid and phenolic content in mg ATE were 5.52±0.07 ㎍ quercetin equivalents and 237.27±13.24 ㎍ gallic acid equivalents, respectively. Antioxidant capacity of ATE determined by DPPH and ABTS assay was increased with a dose dependent manner up to 1000 ㎍/㎖. The amount of melanin synthesis followed by α-melanocyte stimulating hormone on B16F10 cells were significantly reduced in the presence of ATE (250 to 1000 ㎍/㎖, p<0.05). ATE (125 to 1000 ㎍/㎖, p<0.05) suppressed the tyrosinase activity but did not show any significant effect on α-glucosidase activity at the same condition. Taken together, ATE possesses tyrosinase inhibitory potential with significant antioxidant capacities. These effects of ATE might be involved in suppression of melanin synthesis, at least, in B16F10 cells. The anti-melanogenic potential of ATE will provide an insight into developing a new skin whitening product.

The root of Angelica tenuissima Nakai (Umbelliferae) has been used in traditional medicines of Korea as a headache, common cold and a fever remedy. A. tenuissima contains ferulic acid and various compounds of essential oil group such as limonene, 3-butylidenephthalide, γ-terpinene, neocnidilide, ligustilide, senkyunolide and neocnidilide. This study carried out to compare the contents of ferulic acid, z-ligustilide and n-butylidenephthalide between native and cultivated species of A. tenuissima by HPLC. The average contents of ferulic acid, z-ligustilide and n-butylidenephthalide indicated that native species (9 samples) were 0.060%, 0.616%, 0.025% and cultivated species (15 samples) were 0.037%, 0.141%, 0.029%, respectively. All samples were collected from different places in Korea.

This expriment was carried out to get basic information on composition and yield of aromatic constituents in leaves of four medicinal plants, Angelica tenuissima, Chrysanthemum zawadskii. ssp. latilobum, Artemisia iwayomogi and Artemisia capillaris. Volatile aromatic constituents, 28 compounds in Angelica tenuissima were identified and 19 compounds were indentified in Chrysanthemum zawadskii ssp. latilobum. Volatile aromatic constituents, 23 compounds in Artemisia iwayomogi and Artemisia capillaris were identified. Major volatile aromatic consitiuents analyzed by GC/MS in four plants were α-pinene, camphene, sabinene, cis-2-hexanol, and camphor etc. Content of essential oils in Angelica tenuissima, Chrysanthemum zawadskii ssp. latilobum, Artemisia iwayomogi and Artemisia capillaris were 0.014, 0.275, 0.785, and 0.452%, respectively. As a result, it was suggested that a medicinal plant, Artemisia iwayomogi, was worthy of using as a useful material of perfume.