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        검색결과 22

        1.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Background: This study has mainly focused on finding pharmacological effects of ginsenosides that can reduce the unwanted side effects of the cytotoxic anticancer drugs and are highly effective on prostate cancer, colorectal cancer, liver cancer, hormone-dependent breast cancer, triple-negative breast cancer, and brain cancer (neuroblastoma). Methods: Minor and rare ginsenosides (GS) of Rh2 which have a high absorption ability and excellent pharmacological actions were treated with the 6 different types of cancer cell lines and their anticancer activities were investigated by analyzing gene expressions associated with various cancers through qPCR and other relevant methods. Results: In cancer cells exposed to Rh2, cell viability and cell migration were reduced, and apoptosis was induced. Each cancer cell was divided into three groups according to the cell proliferation response by Rh2; 1) A group in which the cell viability decreases inversely to an increase in Rh2 treatment concentration; 2) A group in which the cell viability rapidly decreases in Rh2 treatment above a certain level of concentration; 3) A group in which the cell viability was not suppressed below 20-30% even with 100 μL of Rh2, the highest concentration used in this study. Conclusions: It was shown that Rh2 has a significant effect on inhibiting the proliferation of prostate cancer cells and hormone-dependent breast cancer cells.
        4,300원
        2.
        2022.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In this study, the quality characteristics of kimchi, such as its salinity, pH, and acidity, were measured and compared, and the HT-29 human colon cancer cells were used to show the anticancer effects of kimchi. The kimchi samples used herein included standard kimchi (SK), turnip kimchi (TK), and turnip-powder-added kimchi (TPK). The measured pH and acidity of TK and TPK showed no significant differences with those of SK. Compared to SK and TK, TPK had higher DPPH scavenging activity and higher total flavonoid content, confirming its antioxidant activity. The cancer cell growth inhibition rates of TK and TPK were significantly higher than that of SK. In HT-29 cells treated with TPK, the mRNA expression of Bcl-xL, an anti-apoptosis-related gene, was lower, and the mRNA expressions of the apoptosis-related genes Bax, Bad, and caspase-9 were higher. TPK showed significantly higher levels of mRNA expressions for the cell-cycle-related genes p53 and p21 than the other samples, in addition to suppression effects on cancer cell proliferation. Compared to SK, TK and TPK suppressed the growth of colon cancer cells and showed higher anticancer effects. Therefore, it is shown that kimchi with added turnip powder had high anticancer effects.
        4,000원
        3.
        2022.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Nanomaterials (NMs) are gradually becoming pervasive in the modern world, entering every application for improving the quality of life. Multifaceted uses of NMs in curing diseases, biomedical instrumentation, bioimaging, drugs, and gene delivery, display devices, nanosensors, and biomarkers in several fields ranging from agriculture to industries, healthcare, and environment, have been well recognized. Carbon-based nanomaterials (CNMs) constitute a major type of NMs with broad-spectrum applications including their uses in agriculture. These are synthesized in large quantities via synthetic and biological approaches. Biological approaches are gaining appreciation and momentum, owing to the advantages associated with them, major being their environment friendly or ‘Green’ nature. This topical review focuses on the preparation of CNMs using natural resources, i.e., using the Green Nanotechnology. The up-to-date compilation presented here includes most of the popular green technological methods of producing the CNMs and their immediate uses as anticancer agents, in bio-labelling, as biosensors, in bio-remediation, in cell imaging, in fluorescent inks, and fluorescent dyes, as plant growth inducing agents, in nano-probes, in light-emitting devices and other applications. It is intended to update the reader with the state-of-the-art knowledge about the green technological methods for synthesizing CNMs, their uses, current trends, challenges, and future outlook on the topic.
        6,900원
        5.
        2012.06 구독 인증기관·개인회원 무료
        This study was carried out to investigate anticancer activities fruiting body extracts and fractions of Cordyceps militaris. Fruiting body of this mushroom was extracted using by 80% MeOH. Fractionations of these extracts were performed by n-hexane, methylene chloride, ethyl acetate, n-BuOH. AGS(human gastric cancer line) was cultured in media conditions (10% FBS, 1% Penicillin-Streptomycin in RPMI). Anticancer activities of each fractions of Cordyceps militaris were examined by using MTT, Cell titer Glo.
        6.
        2009.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study is focused on the synthesis of urea and amide derivatives particularly, since the amide moiety is an essential binding group at the binding site. Urea derivatives 3-7 and 13-14 were obtained by reaction of 2-aminopyrimidines and other amines with diverse isocyanates in pyridine as a solvent under reflux. The urea derivatives were obtained in low yield because of the highly electron deficient nature of the amino group of the 2-aminopyrimidine. Amide derivatives 8-10 were obtained in moderate yields by reaction of compound 1 with aryl chloride derivatives. Also, arylamine 11 was synthesized by Buchwald-Hartwig amination in moderate yields. Most of the compound did not show good activity against A375P melanoma cells, compared with Sorafenib as control compound.
        4,000원
        7.
        2020.10 KCI 등재 서비스 종료(열람 제한)
        본 연구 결과들을 바탕으로 도깨비부채 잎(RPL)은 GSK3β 활 성화를 통해 IκB-α를 인산화시켜 단백질 분해를 유도하고 Iκ B-α 분해로 인해 p65 핵내 전이를 유도하여 NF-κB 신호전달을 활성화?시킨다. 이러한 NF-κB 신호전달 활성화를 통해 대장암 의 세포생육을 억제하는 것으로 추정된다. 본 결과는 도깨비부채 잎을 소재로 항암을 목적으로 한 천연치료제 및 대체보완소재 개발에 활용할 수 있다고 판단된다. 그러나 도깨비부채 잎의 대장암에 대한 세포생육 억제와 작용기전의 정확한 관련성과 세포생육 억제활성 물질 분석을 위해 추가적인 연구가 필요할 것으로 사료된다.
        8.
        2020.08 KCI 등재 서비스 종료(열람 제한)
        이상의 연구 결과로 미루어 볼 때, 댕댕이나무 잎과 가지 추추출물은 대장암 세포주 HCT116과 SW480세포의 생육을 억제 하였으나 열매추출물은 억제활성이 나타나지 않았다. 잎과 가지 추출물은 cell migration과 wound healing assay를 통해 비정상적인 세포증식 억제를 확인하였으며, β-catenin과 TCF4 의 단백질 수준을 감소시켜 비정상적인 Wnt 신호전달을 억제를 통해 대장암세포의 생육을 억제하는 것으로 판단된다. 따라서 댕댕이나무 잎과 가지는 항암을 위한 대체보완소재 및 천연 항암제 개발을 위한 소재로 활용이 가능할 것으로 판단된다.
        9.
        2019.10 KCI 등재 서비스 종료(열람 제한)
        이상의 연구 결과로 미루어 볼 때, 도깨비부채 잎(RPL)은 β -catenin의 분해 유도를 통해 대장암, 유방암, 폐암, 전립선암 및 췌장암 세포의 생육을 억제하는 것으로 나타났다. 본 결과는 도깨비부채 잎의 항암을 위한 대체보완소재 및 천연 항암제 개발을 위한 소재로 활용이 가능할 것으로 판단된다. 그러나 추가적 연구를 통해 도깨비 부채 잎의 항암 활성물질의 분석연구가 필요할 것으로 사료된다.
        10.
        2019.04 KCI 등재 서비스 종료(열람 제한)
        In this study, we evaluated the effect of branch (STB) and leave (STL) extracts from Sageretia thea on β-catenin level in human colorecal cancer cells, SW480 and lung cancer cells, A549. STB and STL dose-dependently suppressed the growth of SW480 and A549 cells. STB and STL decreased β-catenin level in both protein and mRNA level. MG132 decreased the downregulation of β-catenin protein level induced by STB and STL. However, the inhibition of GSK3β by LiCl or ROS scavenging by NAC did not block the reduction of β-catenin protein by STB and STL. Our results suggested that STB and STL may downregulate β-catenin protein level independent on GSK3β and ROS. Based on these findings, STB and STL may be a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer and lung cancer.
        11.
        2019.04 KCI 등재 서비스 종료(열람 제한)
        In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of branches from Taxillus yadoriki parasitic to Neolitsea sericea (TN-NS-B) against human lung cancer cells, A549. TY-NS-B dose-dependently suppressed the growth of A549 cells. TY-NS-B decreased β-catenin protein level, but not mRNA level in A549 cells. The downregulation of β-catenin protein level by TY-NS-B was attenuated in the presence of MG132. Although TY-NS-B phosphorylated β-catenin protein, the inhibition of GSK3β by LiCl did not blocked the reduction of β-catenin by TY-NS-B. In addition, TY-NS-B decreased β-catenin protein in A549 cells transfected with Flag-tagged wild type β- catenin or Flag-tagged S33/S37/T41 mutant β-catenin construct. Our results suggested that TN-NS-B may downregulate β- catenin protein level independent on GSK3β-induced β-catenin phosphorylation. Based on these findings, TY-NS-B may be a potential candidate for the development of chemopreventive or therapeutic agents for human lung cancer.
        12.
        2018.06 KCI 등재 서비스 종료(열람 제한)
        In this study, we investigated the effect of the extracts from Vaccinium oldhamii on cell proliferation and the regulatory mechanisms of cyclin D1 protein level in human cancer cells. The branch extracts from Vaccinium oldhamii (VOB) showed higher inhibitor effect against the cell growth than leave extracts (VOL) and fruit extracts (VOF) in human colorectal cancer, breast cancer, prostate cancer, non-small lung cancer, pancreatic cancer and liver cancer cells. In addition, VOB decreased cyclin D1 level at both protein and mRNA level. MG132 treatment attenuated VOB-mediated cyclin D1 downregulation. A point mutation of threonine-286 to alanine attenuated cyclin D1 degradation by VOB. In addition, the inhibition of nuclear export by leptomycin B (LMB) attenuated cyclin D1 degradation by VOB. But, the treatment of PD98059 (ERK1/2 inhibitor), SB203580 (p38 inhibitor), SP600125 (JNK inhibitor), LiCl (GSK3β inhibitor), LY294002 (PI3K inhibitor) or BAY 11-7082 (IκK inhibitor) did not affect VOB-induced cyclin D1 degradation. In conclusion, VOB induced cyclin D1 degradation through redistribution of cyclin D1 from the nucleus to cytoplasm via T286 phosphorylation of cyclin D1, which resulted in the inhibition of cancer cell proliferation.
        13.
        2017.12 KCI 등재 서비스 종료(열람 제한)
        In this study, we elucidated anti-cancer activity and potential molecular mechanism of 70% ethanol extracts from Taxilli Ramulus (Taxillus chinensis (DC.) Danser) (TR-E70) against human colorectal cancer cells. Anti-cell proliferative effect of TR-E70 was evaluated by MTT assay. The effect of TR-E70 on the expression of cyclin D1 in the protein and mRNA level was evaluated by Western blot and RT-PCR, respectively. TR-E70 suppressed the proliferation of human colorectal cancer cell lines, HCT116 and SW480. Although TR-E70 decreased cyclin D1 expression in protein and mRNA level, decreased level of cyclin D1 protein by TR-E70 more dramatically occurred than that of cyclin D1 mRNA. Cyclin D1 downregulation by TR-E70 was attenuated in presence of MG132. In addition, TR-E70 phosphorylated threonine-286 (T286) of cyclin D1. TR-E70-mediated cyclin D1 degradation was blocked in presence of LiCl as an inhibitor GSK3β but not PD98059 as an ERK1/2 inhibitor and SB203580 as a p38 inhibitor. Our results suggest that TR-E70 may downregulate cyclin D1 as one of the potential anti-cancer targets through GSK3β-dependent cyclin D1 degradation. From these findings, TR-E70 has potential to be a candidate for the development of chemoprevention or therapeutic agents for human colorectal cancer.
        14.
        2016.06 KCI 등재 서비스 종료(열람 제한)
        The seed of safflower (Carthamus tinctorius L) has been reported to suppress human cancer cell proliferation. However, the mechanisms by which safflower seed inhibits cancer cell proliferation have remained nuclear. In this study, the inhibitory effect of the safflower seed (SS) on the proliferation of human colorectal cancer cells and the potential mechanism of action were examined. SS inhibited markedly the proliferation of human colorectal cancer cells (HCT116, SW480, LoVo and HT-29). In addition, SS suppressed the proliferation of human breast cancer cells (MDA-MB-231 and MCF-7). SS treatment decreased cyclin D1 protein level in human colorectal cancer cells and breast cancer cells. But, SS-mediated downregulated mRNA level of cyclin D1 was not observed. Inhibition of proteasomal degradation by MG132 attenuated cyclin D1 downregulation by SS and the half-life of cyclin D1 was decreased in SS-treated cells. In addition, SS increased cyclin D1 phosphorylation at threonine-286 and a point mutation of threonine-286 to alanine attenuated SS-mediated cyclin D1 degradation. Inhibition of ERK1/2 by PD98059 suppressed cyclin D1 phosphorylation and downregulation of cyclin D1 by SS. In conclusion, SS has anti-proliferative activity by inducing cyclin D1 proteasomal degradation through ERK1/2-dependent threonine-286 phosphorylation of cyclin D1. These findings suggest that possibly its extract could be used for treating colorectal cancer.
        16.
        2011.02 KCI 등재 서비스 종료(열람 제한)
        본 연구에서는 척리 즉 물이끼를 이용한 고부가가치 기능성 소재를 개발하기 위하여 물이끼의 Ethanol 추출물을 이용한 항암활성과 식물화학적 성분연구를 수행 하였다. 3종의 암세포주(A549, HeLa, SK-OV-3)에 대하여 세포독성연구를 수행해 본 결과 폐암세포주인 A549에서 유의성 있는 결과를 보였다. 이 결과는 난치성 질환인 암을 치료하려는 목적 의약품 보다는 Chemopreventive agent로서의 예방 의학적 기능성 소재로 충분한 가치가 있음이 사료 되어지기에 식물화학적 성분연구를 실시하여 7종의 화합물(Comp.1 : Coumarin, Comp.2 : Caffeic acid, Comp.3 : Quercetin, Comp.4 : Astragalin, Comp.5 : Luteolin, Comp.6 : Chlorogenic acid, Comp.7 : Rutin) 를 분리하여 구조동정 하였다.
        17.
        2010.06 KCI 등재 서비스 종료(열람 제한)
        We investigated a method to improve anticancer activities of Acer mono wood extracts by ultra high pressure extraction process. The A. mono was extracted by water at 40℃ and 300 MPa for 15 min (High Pressure Extraction, HPE). The extraction yield by ultra high pressure extraction process was 5.42%. The cytotoxicity on human normal lung cell (HEL299) of the extracts from HPE showed 21.54% lower than that from conventional water extraction at 100℃ in adding the maximum concentration of 1.0 mg/ml. Ultra high pressure extracts process for 15 minutes extracts (HPE15) showed more potent scavenging effect than the control, BHA. On SOD-like test, the HPE15 showed highest activity as 32.4% at 1.0 mg/ml concentration. Human stomach adenocarcinoma, liver adenocarcinoma, breast adenocarcinoma and lung adenocarcinoma cell growth were inhibited up to about 67~79%, in adding 1.0 mg/ml of extracts from HPE. HPE was 20~25% higher than conventional water extraction. It was interesting that, among several cancer cell lines (stomach adenocarcinoma, liver adenocarcinoma), the growth of digestive related cancer cells were most effectively inhibited as about 75~79%. On in vivo experiment using ICR mice, the variation of body weight of mice group treated A. mono wood extracts from HPE of 100 mg/kg/day concentration was very lower than control and other group. The survival times of group treated this extracts was 61.96% longer than that of the control group and this extracts showed the lower tumor weight, which were 10.49 g than positive control as 16.17 g. Based on these results, we could tell that the HPE wood extracts of A. mono had higher anticancer activity than conventional water extraction. The results of HPE showed obvious advantages in higher efficiency, shorter extraction time, at lower energy costs.
        18.
        2007.10 KCI 등재 서비스 종료(열람 제한)
        The potential antioxidant and anticancer activities of Hexane, EtOAc (Ethyl acetate), BuOH (n-Buthanol) and water fractions from methanolic (MeOH) extract of Picrasma quassioides (D. Don) Benn. were evaluated in vitro. Tested fractions showed strong antioxidant activity, especially EtOAc fraction had the highest activity (IC50 = 114.01 μg/mL), containing high total phenolics and total flavonoids contents, showed 67.59 Tan μg/mg and 64.95 Que μg/mg respectively. Anticancer activity of these fractions was tested by MTT assay on HT-29 (the human colon carcinoma cells) cell line. BuOH fraction not only showed very high anticancer activity, but also had no cytotoxic effect on 293 (the human normal kidney cells) cell line. Considering these results, we used BuOH fraction of MeOH crude extract from P. quassioides (D.Don) Benn. to do assessment of apoptosis by flow cytometry and the mRNA expression levels of widely established apoptotic-related genes on HT-29 cell line. All the experiments showed that BuOH fraction can induce apoptosis on HT-29 cell line strongly. Taken together, methanolic extract of P. quassioides has potential for antioxidant and anticancer activities products.
        19.
        2006.12 KCI 등재 서비스 종료(열람 제한)
        Tocotrienol의 건강식품 및 의료용 가공품으로의 고부가가치화 가능성을 평가하기 위하여 미강에서 추출한 tocotrienol의 항암 효과 및 독성평가를 실시하였다. HepG2 간암 세포를 대상으로 한 항암효과 분석 결과 tocotrienol이 간암세포의 증식을 억제함을 입증할 수 있었다. 또한 랫드를 이용한 경구독성 검사를 수행한 결과 tocotrienol 최종 생산물은 경구투여 한계량인 2,000mgkg1 수준에서도 사망률, 일반 증상, 체중 변화 등에 이상을 보이지 않았으며, 부검 후 흉강 및 복강의 모든 장기에 대한 육안적 부검 소견 등에서도 아무런 독성학적 변화가 유발되지 않음을 알 수 있었다.
        20.
        2005.04 KCI 등재 SCOPUS 서비스 종료(열람 제한)
        양파고추장에 대한 항암 및 면역활성을 조사한 결과는 다음과 같다. 고추장 메탄을 추출물은 aflatoxin 으로 돌연변이를 유도한 Salmonella typhimurium에 대하여 농도 의존적으로 항돌연변이 효과가 큰 것으로 나타났으며, 양파를 첨가하지 않은 대조구 고추장보다 양파고추장이 그 효과가 더욱 크게 나타났다. 양파고추장 메탄올추출물은 A549 및 MCF-7 암세포주에 처리한 결과 대조구에 비하여 농도에서 모두 이상 그 성장을 억제하였
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