목 적: 시력교정용 안경의 진균 오염실태를 조사하고자 하였다. 방 법: 총 145명(초등학생 36명, 중학생 37명, 고등학생 30명, 대학생 10명, 노인 32명)의 안경으로부터 진균을 채취하여 분리 배양한 후 동정하였다. 결 과: 시력교정용 안경으로부터 검출된 진균은 총 5종으로 Penicillium sp., Cladosporium sp., Aspergillus sp., Mucor sp., Cryptococcus neoformans이었다. 안과질환과 관련한 진균류는 Aspergillus sp., Cladosporium sp., Penicillium sp.로 3종이었으며 그 외 질병의 원인균은 Mucor sp., C. neoformans로 2종이었다. 결 론: 시력교정용 안경에서 고위험군 감염성 진균류들이 존재하는 것을 확인하였으며 이를 통한 질병의 유발이 예측되므로 안경의 위생관리를 위한 대안이 필요하다고 사료된다.

본 연구는 polyvinyl chroride (PVC)와 stainless steel 표면에서 E. coli O157:H7의 부착, 바이오필름형성, 온·습도에 따른 생존율을 조사하고, E. coli O157:H7이 오염된 작업대 표면에서 상추로의 교차오염도 조사를 통하여 E. coli O157:H7에 오염된 작업대가 상추의 미생물학적 안전성에 미치는 영향을 평가하고자 수행하였다. 작업대 표면 재질인 PVC와 stainless steel에 E. coli O157:H7 배양액을 1시간 노출시켰을 때, PVC의 경우, stainless steel 보다 10배 정도 부착력이 높았으나, 6시간째는 두 재질간의 차이가 없었다. 또한, E. coli O157:H7의 바이오필름 형성은 TSB > 10% 상추추출액 > 1% 상추추출액 > phosphate buffer 순이었으며, 재질별로는 PVC에서 바이오필름형성능이 높은 것으로 나타났다. 온도 20oC, 30oC, 습도 43%, 69%, 100% 에서 각각 노출 시켰을 때, E. coli O157:H7은 온도 30oC, 습 도 43%, 69% 조건에서 12시간 내에 약 5.0 log CFU/coupon이 감소한데 반해, 상대습도 100%에서는 농도의 큰 변화 없이 5 일 이상 생존이 지속되었다. 또한, 유기물이 작업대 표면에 존재 시, E. coli O157:H7의 감소 속도가 느렸다. E. coli O157:H7 에 오염된 작업대에 상추를 접촉시키고 상추의 오염도를 조사한 결과, 상추 표면에 수분이 존재할 때 오염된 작업대 표면에서 E. coli O157:H7의 이동수준이 상추에 수분이 없을 때 보다 10 배 이상 증가하는 것으로 나타났다. 따라서 E. coli O157:H7에 오염된 작업대는 상추의 안전성에 직접적인 영향을 미칠 수 있어 작업 후에 세척, 소독을 통하여 위생적으로 관리하는 것이 필요하다.

본 연구에서는 토마토의 생산 및 유통단계에서의 위해 미생물 오염도 정도를 파악하고 유통과정 중 외부 손상으로 인해 과즙 유출시 미생물의 증식능력을 측정하기 위해 E. coli O157:H7와 L. monocytogenes의 성장을 토마토 추출물 함량(0.1, 1.0, 10%) 및 다양한 배양온도(5, 15, 25, 35oC)에서 측정하였다. 수확단계에서는 APC 작업 종사자의 장갑에서 총 호기성 세균이 7.77 log로 가장 높게 나타 났고, 배양 양액에서 대장균군과 B. cereus가 각각 0.33log 검출되었다. 수확 후 처리과정으로 APC 과정을 거친 토마토에서 총 호기성 세균이 유의적으로 높게 나타났다. 그 외에 S. aureus, Salmonella spp.는 검출되지 않았다. 재배방법에 따른 일반, 유기농, 무기농 토마토에서의 미생물 분포도의 유의적인 차이는 나타나지 않았다. 포장 방법 중 봉지 포장제품이 박스 포장제품에 비해 대장균군이 유의적으로 높게 나타났으며 세척에 의한 미생물 저감화 효과는 나타나지 않았다. 토마토 추출물 함량이 높을수록 E. coli O157:H7와 L. monocytogenes의 성장이 높게 나타 났으며, 토마토 추출물 10%인 경우 5oC 조건에서 가장 낮았고, 15, 25, 35oC에서 72시간 배양시 각각 7.33~8.51, 7.73~8.60 log CFU/g으로 증식하였다.

Influences of low concentration of heavy metals (As, Cd, Cu and Pb) on the nematode community structure were examined to assess the changes of the soil ecosystem for a short period of time. Notable increases of heavy metal concentration on the experiment soil were found on the maximum and their 4-1x concentration treatment of all heavy metals after 18weeks. Overall abundance of the nematodes increased regardless of kinds of heavy metal and their concentrations. Also there were no significant differences in the nematode abundance among all treatments of heavy metals except the abundance of c-p 4 in the maximum treatment of Pb. The number of nematode genus found in soils treated with heavy metal solutions also increased compared to that of the genus of the initial soils; however, no significant differences in richness were observed among the treatments of all heavy metals. In maturity and diversity indices of the nematode community, no significant changes occurred in the soils treated with heavy metal solutions with a few exceptions such as MI2-5 and ΣMI2-5 for Pb, and MI2-5 for As. Significant decreases of the ecological indices in the treatments with highly concentrated heavy metal solutions were noticed in MI2-5 and ΣMI2-5 for Pb, and MI2-5 for As, respectively. All of these results is supposed that the maturity and diversity of the nematode community may be decreased in soils contaminated with heavy metals such as Pb and As at relatively low concentrations of a short period of time, which may be derived from differential effects of heavy metals at low concentrations on the growth and development of the nematodes with different c-p values and trophic types. In the analysis of food web structure, only structure index (SI) was significantly lowered in soils irrigated with the heavy metal solutions of Pb and As, but enrichment index (EI) and channel index (CI) were not, suggesting that stressful soil conditions might be provided to the soil food web structure governed by direct toxic effects of heavy metals differential to nematode populations with different trophic type sandc-p values. Analyses of correlation coefficients also suggest that the influence of heavy metals at low concentrations, especially by Pb, maybe most prominent on the nematodes of c-p2-5, including fungivores, providing stressful soil environments.

본 연구는 국내 생산 볏짚의 곰팡이독소 오염 실태를 파악하기 위하여 벼의 생육 단계별, 볏짚의 보관 형태별 및곤포사일리지의 지역별 오염을 조사하였다. 수확하기 전논에서 생장 중인 벼에서 곰팡이독소 및 곰팡이 오염이 확인되었다. 벼의 생장 초기에는 deoxynivalenol (297 μg/kg)이발견되었고, 중기에는 ochratoxin A (1.8 μg/kg) 및 deoxynivalenol(264 μg/kg)이 오염되어 있었다. 그리고 수확시기에는 deoxynivalenol (190 μg/kg) 및 zearalenone (168 μg/kg)이오염되어 있었다. 그리고 곰팡이 오염은 생장 초기에는 103－4cfu/g 그리고 생장 중기와 수확시기에는 104－5 cfu/g 이었다.오염된 곰팡이로는 Fusarium sp., Fusarium proliferatum,Penicillium sp., Gibberella sp., Gibberella zeae, Mucorcircinelloides, Aspergillus oryzae 등 다양한 종류가 발견되었다. 사료용 볏짚의 통 볏짚단, 사각 볏짚단 및 곤포사일지 등 저장 방법에 따른 오염된 mycotoxin을 조사하였을때 zearalenone, deoxynivalenol 및 ochratoxins A가 검출되었다. 통 볏짚단의 곰팡이독소 오염도는 23%이었고, 사각볏짚단과 곤포사일지지는 각각 38%가 오염되어 있었다. 그리고 105점의 볏짚 곤포사일리지에서 곰팡이독소를 분석하였을 때 46%의 샘플에서 곰팡이독소가 발견되었고, 지역적으로는 경기, 강원, 충청, 영남 및 호남지역에서 각각 48,33, 40, 50 및 57%의 샘플이 오염되어 있었다. 2가지의 곰팡이독소(zearalenone, deoxynivalenol)가 발견된 충청지역을제외한 모든 지역의 볏짚 곤포사일리지에서 3가지의 곰팡이독소(zearalenone, deoxynivalenol, ochratoxins A)가 발견되었다. Ochratoxins A의 오염 수준은 전체적으로 2.6 μg/kg으로 충청지역에서는 발견되지 않았지만 호남지역은 3.0μg/kg으로 다른 지역보다 높았다. Deoxynivalenol의 오염수준은 전체적으로 413 μg/kg으로 충청지역이 800 μg/kg로가장 높았고, 다른 지역은 246 μg/kg에서 433 μg/kg의 수준으로 발견되었다. 그리고 zearalenone은 전체적으로 338 μg/kg으로 경기지역이 168 μg/kg로 다른 지역보다 낮았고, 강원과 호남지역이 각각 467 μg/kg과 442 μg/kg으로 높았다.이상의 연구 결과는 볏짚은 수확하기 전 벼의 생장 기간에도 곰팡이독소 및 곰팡이 오염에 노출되어 있으며, 볏짚의보관 형태 및 지역에 상관없이 곰팡이독소의 오염이 발생할 수 있음을 명확히 시사한다. 따라서 안전하고 질 좋은조사료를 확보하기 위해서는 이들 곰팡이독소 관련 더 구체적 연구와 대책관련 탐구가 지속적으로 이루어져야 할것으로 사료된다.

This study was conducted to monitor and evaluate microbial contamination during manufacturing process in 6 red pepper powder factories. Red pepper powder samples were taken from manufacturing facilitates, working area and workers’ hands to determine sanitary indicator bacteria (SIB) such as aerobic bacteria and coliform group as well as pathogenic indicator bacteria (PIB) such as Staphylococcus aureus, E.coli, Salmonella spp., Listeria monocytogenes, and Bacillus cereus. The results indicated that SIB in primary materials was detected as low as 3 log units and E.coil and Staphylococcus aureus of PIB were detected. After grinding process, aerobic bacteria, fungi, and coliform group increased 52% and 108%, respectively. In final products, PIB was not detected except for one found Staphylococcus aureus by which workers’ hands were contaminated. Moreover, UV detectors in all the manufacturers were not able to reduce bacteria. Thus, this data suggest that a stringent safety management be needed to prevent cross contamination, and also reconsider effectiveness of facility.

The objective of this study was to investigate and evaluate microbial contamination levels of leafy greens (perilla leaf and lettuce) and its distributing conditions at different seasons (Feb, May, Aug, and Nov of the year 2011) in order to provide insight into any potential health hazards associated with consumption of these commodities. Leafy greens were collected from a farm located in Geumsan, Chungnam and wholesale markets (WM) and traditional markets (TM) located in Suwon. At the same time, temperature and relative humidity fluctuations experienced by the leafy greens during distribution from the farm to the distribution center were measured by a data logger. The contamination levels of perilla leaf and lettuce were determined by analyzing total plate count. Coliform groups, Bacillus cereus, Escherichia coli, Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes and Staphylococcus aureus were determined. The contamination levels of total aerobic bacteria, coliform groups and B. cereus in both vegetables sampled during May and August found to be higher than those sampled during February and November. E. coli O157:H7, Salmonella spp., L. monocytogenes were not detected in the vegetables analyzed in this study. There were no significant trends between samples at WM and TM in the contamination levels. Relative humidity of vegetables distributed from the farm to the distribution center showed over 90% during distribution regardless of measured seasons. In the case of background microflora on leafy greens, the density was significantly increased at 20, 30 and 37oC during storage of 24h. E. coli O157:H7 and B. cereus inoculated on the leaves also showed similar increases in the storage tests. The microbial contamination levels determined in this study may be used as the fundamental data for microbial risk assessment.

The purpose of this study was to evaluate microbiological contamination of fresh-cut produce salads and raw cabbage toward climate change. Total aerobic bacteria, coliform and Escherichia coli were monitored to get the contamination levels and E. coli O157:H7, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes and Salmonella spp. to detect pathogens with risk of foodborne disease from samples. Collection of 360 samples (180 fresh-cut produce salads and 180 raw cabbage), including 60 samples from each area after setting 3 areas depending on annual temperature and annual rainfall. As a result, total aerobic bacteria and coliform group were different was performed areas in raw cabbage but there was no difference between areas in fresh-cut produce salads. In addition foodborne pathogens including E. coli were not isolated from fresh-cut produce salads.

This study was conducted to evaluate microbial contamination levels of Korea traditional rice cakes such as Sirutteok, Garaetteok and Gyeongdan in the manufacturing process and environment. The microbial contamination levels such as total aerobic bacteria, fungi, coliforms, Escherichia coli, Staphylococcus aureus, Bacillus cereus and Clostridium perfringens of rice cake products were analyzed. The contamination levels of total aerobic bacteria, coliforms, fungi and B. cereus in raw materials were in the range of 2.4~4.5, ND~1.9, 1.2~2.1 and 1.0~2.1 log CFU/g, respectively. The microbial contamination levels of total aerobic bacteria, coliforms, fungi and B. cereus in manufacturing process of rice cakes were increased in the soaking and grinding steps and were decreased in steaming step. E. coli, S. aureus and C. perfringens were not detected in any manufacturing process and environment. The microbial contamination levels of raw materials and final products of rice cake were suitable for microbial safety standard in Korea. However, the manufacturing environment such as equipments and employee's sanitation were in trouble for microbial safety. The results of this study suggest that safety education for personal hygiene and safety management in processing environment are continuously required to assure safety in working environment and employee's individual hygiene.

This study was investigated to determine the contamination levels of total aerobic bacteria, coliform group, E. coli and food-borne pathogens of side dishes from 2 traditional markets (100 samples) and 2 super markets (100 samples) located on Ulsan. The levels (range) of total aerobic bacteria was 4.75 log CFU/g (1.60~6.92 log CFU/g) in traditional market and 4.62 log CFU/g (2.00~6.46 log CFU/g) in super market, respectively. Coliform was detected in 64 and 66 samples sold at traditional markets and super markets, respectively. E, coli was detected in 4 and 6 samples sold at traditional markets and super markets, respectively. The food-borne pathogens, namely Bacillus cereus and Listeria monocytogenes were detected in 1 sample sold at traditional markets, respectively, and Bacillus cereus was detected in 4 samples sold at super markets. However, other pathogens such as Salmonella spp., Shigella spp., Vibrio parahaemolyticus, Yersinia enterocolitica, Clostridium perfringens, Camphylobacter jejuni and Pathogenic E. coli were not detected. The Saengchae and Seasoned Jeotgal were relatively vulnerable compared to the others in the food-borne pathogens.

본 연구에서는 배양된 액체종균의 세균 오염 여부를 Giemsa 용액의 단일 염색으로 짧은 시간 안에 정확하게 판정이 가능한지를 조사하였다. Giemsa 용액은 혈액·골수·림프절·말라리아 원충·리케차 세포 등을 염색하는 것으로, 염기성 색소인 methylene azul과 methylene blue, 그리고 산성색소인 eosine을 methyl alcohol-glycerine에 녹여서 제조하였다. 그리고 팽이 액체종균의 폭기 배양액 분취하여 슬라이드에 올리고 Gimesa 용액으로 염색한 후 광학 현미경으로 검사하였다. 이 결과 40~60초 동안의 세균세포를 (팽나무)버섯(류) 균사세포의 부스러기나 잔존 대두박 등과 구별할 수 있었다. 이 Gimesa 용액을 이용한 염색 및 검경방법은 빠르고 간편하며 정확하므로 액체종균을 사용하는 버섯재배 농가에서도 세균 오염을 효과적으로 동정(감지)하는데 이용할 수 있다고 생각한다.

This study was performed to determine the transfer rates of each foodborne pathogen from pork meat packaging during the processing. We analyzed the transfer rate of Listeria monocytogenes from contaminated pork meat to worker's hands (wearing polyethylene gloves, PEG; cotton gloves, CG; and bare hands), cutting boards and knives, and vice versa. Transfer rate of CG 100.00% was higher than that of bare hands 2.513% and PEG 1.511%. In particular, when wearing CG, the transfer rate from the CG to bare hands with CG was 0.08%. Also, the range of transfer rates from contaminated pork meat to cutting board and knife was 0.352-3.791%. In contrast, transfer rates from the workers’ hands (with PEG/CG and bare hands) to cutting board, knife, and pork meat ranged from 0.001 to 0.141%. There was a lower transfer rate from workers’ hands than from pork meat. These findings indicate that use of PEG could effectively reduce or prevent the cross-contamination compared to CG and provide important information concerning the consecutive transfer of L. monocytogenes during food processing.

The objective of this study was to determine the effects of E. coli isolated from porcine semen on sperm viability, motility, and semen pH. Semen samples were prepared using commercial extender, SeminarkPro (Noahbio Tech, Korea) that did not contain antibiotics. And 4 different levels of E. coli were artificially innoculated to semen with following concentrations; 4,000 of sperms with 1 of E. coli (T1), 400 with 1 (T2), 40 with 1 (T3), and 4 with 1 (T4). Semen samples were preserved at 17℃ for 5 days in semen storage box until analyzed by flowcytometer. Aliquots were subjected to measure the sperm viability (Live/Dead® stain), motility (mitochondrial function), and semen acidity (pH) from day 0 (day of semen collection) to day 5. Sperm motility and viability were significantly decreased (p<0.05) on day 0 (4 hrs after preservation at 17℃) in T3 and T4 compared to control groups and were significantly decreased (p<0.05) in all groups from day 3. Sample pH was acidic in T3 (6.90～6.86) and T4 (6.86～6.65) from day 3 to day 5 (p<0.05). On the other hand, sample pH was maintained 7.0～7.1 in control, T1, and T2 during the experimental period. Sperm motility and viability were significantly decreased from day 0 to day 5 compared to control in samples contaminated with E. coli above a value of 40:1 (20×106 sperm cells/ml : 5×105 cfu/ml). Even on day 1 in T4 and on day 3 in T3, semen pH was acidic probably due to the acidification of dead spermatozoa. These results suggest that E. coli contamination has a concentration-dependent detrimental effect on extended porcine semen quality.

본 연구는 국내 생산 사료용 볏짚 곤포사일리지의 수확 후 저장기간 동안 생성된 곰팡이 오염도 및 곰팡이 종류를 조사하고 mycotoxin 노출 유해성을 구명하고자 실시하였다. 곰팡이 및 mycotoxin 오염을 조사하기 위하여 곰팡이 발생이 육안 식별이 안 되는 정상적인 것부터 오염이 심한 것까지 다양한 상태의 농가에서 급여하고 있는 33점의 볏짚 곤포사일리지 시료를 수거하여 조사하였다. 오염된 곰팡이는 흰색, 회색, 푸른색 및 검은 갈색 등 다양한 색

Deoxynivalenol (DON) and zearalenone (ZEN) are mainly contaminated mycotoxins in feeds. The study was carried out to analyze and survey the contamination of DON and ZEN in one hundred thirteen samples of feeds. After cleaning all samples with immunoaffinity column, the mycotoxins were analysed by using high performance liquid chromatography/fluorescence with diode array detector (HPLC /FLD with DAD). The average recoveries of DON were 88.76 and 95.40% at the levels of 200 and 1,000 μg/kg and 87.09 and 98.40% of ZEN were recovered at the levels of 100 and 500 μg/kg, respectively. The limit of detection (LOD) were 6.0 and 3.0 μg/kg for DON and ZEN, respectively. The average concentrations of DON were 372.1, 324.0 and 990.9 μg/kg in chicken, pig and cattle feed, respectively. Those of ZEN were 76.1, 43.7 and 196.2 μg/kg for them, individually.

Bacterial contamination of beach sand was examined in April, June, July, and August. Twenty four topsoil and subsoil samples were taken from the 1m and 5m points from the coastline in three Busan beaches (Haeundae, Gwanganli, and Songjeong). The 5m points

목적: 500 mL 용량의 식염수가 한국에서는 콘택트렌즈 헹굼에 흔히 사용되고 있기 때문에 본 연구에서는 콘택트렌즈 착용자가 사용한 대용량 식염수의 미생물 오염과 관련된 위험 요인을 분석하였다. 방법: 120명의 소프트콘택트렌즈 착용자를 모집하고 식염수를 나누어 준 후 이들이 사용한 식염수를 수집하여 분석하였다. 사용한 식염수의 미생물 오염상태는 세균의 집락수 (colony-forming units)로 평가하였고, 세균의 종류는 유전자 중합효소반응(Polymerase Chain Reaction, PCR)으로 얻은 DNA 서열을 자동 염기서열분석기 및 BLAST 분석기를 이용하여 동정하였다. 식염수 오염에 영향을 미치는 요인은 카이제곱검정, 상관분석 및 다중회기분석으로 분석하였다. 결과: 수집된 식염수 중 45%가 세균에 오염되었으며 7종의 감염성 세균을 포함한 27종의 세균이 검출 되었다. 세균의 오염은 무방부제 식염수, 구멍이 뚫린 용기, 식염수 사용기간 및 렌즈착용 전의 손 세척 습관과 상관성이 있었다. 결론: 방부제가 포함된 식염수는 구멍이 뚫려있지 않은 용기에 적은 용량으로, 무방부제 식염수는 1회용으로 제조되어야 할 것이며, 콘택트렌즈 착용자에게는 관리방법에 대한 재교육이 필요할 것으로 사료된다.