In this study, we established the pre-treatment conditions that could increase the γ-aminobutyric acid (GABA) content during barley germination. In the process, three different barley samples were prepared, which differed in the pre-treatedment processes. The specimens were stored at 50 for 1 h after being kept in water at room temperature for 4 h (HKW sample), kept in sufficient water for 4 h (KW sample), or left untreated (CO sample). After the pre-treatment, the barely samples were germinated for 35 h. A sample was taken from each batch in 5-h intervals, extracted with water, and physicochemical characteristics and radical scavenging activity were measured. As a result, we found that the contents of phenolic compounds (18.02-30.63 mg/100 g) and flavonoids (1.87-4.63 mg/100 g) were higher in HKW, showing similar trends. Also the GABA contents in the HKW and KW samples were higher than that in the CO sample. Furthermore, the radical scavenging activities of DPPH and ABTS were the highest in the HKW sample, having values in the ranges of 58.49-77.40% and 54.57-88.10%, respectively. All in all, we found that in order to increase the antioxidative activity and GABA content of the barley samples, it would be suitable to pre-treatment the specimens after the post-immersion heat treatment. In addition, pre-treating the KW samples is appropriate only after immersion time. Lastly, the optimum germination time of the batches was found to be 20-25 h.

Background : Licorice (Glycyrrhiza uralensis F.) is one of the most widely used raw materials for food and pharmaceuticals, and is currently the No. 1 imported medicinal plant in Korea. Efforts to settle licorice cultivation in Korea have been carried out for hundreds of years but have not succeeded, and many people suspect that the reason is due to the inadequate cultivation environment. However, even until recently, most licorice has been procured by wild harvesting, so studies on licorice cultivation technique and cultivation environment have not been conducted much in the world. This experiment was conducted to investigate the germination characteristics of licorice by temperature and salt concentration in order to find the optimal cultivation environment conditions of licorice. Methods and Results : The germination rate of licorice seeds was high 77.5 - 90.5% at 10 - 3 6℃, but it started to drop from 66.5 - 68.0% at 38 - 40℃ and it decreased sharply from 9.0 - 15.0% at 42 - 46℃. No longer germinated at temperatures above 48℃. The average number of days of germination was 12.3 days at 10℃, 5.7 days at 20℃, 3.5 days at 30℃ and 4.1 days at 40℃. The germination start date was 2 to 4 days in the 18℃ - 46℃ range. The average germination rate was the highest at 32℃. The germination time of 40% was 2 - 4 days at 18 - 46℃. The germination of more than 80% took 3 - 5 days at 20 - 40℃, 6 - 8 days at 14 - 1 8℃ and 10 days at 10 - 12℃. Licorice was able to germinate even in the presence of salt. Seed germination rate was 78.5 - 85.5% at 0 - 1% salt concentration and 63% at 1.5% and 18% at 2.0%. In addition, 8.5% seeds germinated successfully even at 3.0% salt concentration. Conclusion : Licorice has been known as a low temperature crop, but germination was possible in a wide range of temperature and salt concentration. Especially, the germination characteristic was higher in the high temperature range of 30 - 36℃, which corresponds to the domestic summer temperature. Therefore, the results of this study are expected to be useful reference for exploring suitable cultivation area in Korea.

This research was performed to develop mass propagation method of Alpine modest primrose (Primula modesta var. hannasanensis T.Yamaz.) for improving the conservation and utilization of the species. Seeds were collected on August 2011 in Jeju-city and well-selected seeds were dry-stored at 4 ± 1.0℃. Seed size ranged 0.44 ± 0.07 × 0.61 ± 0.04 ㎜, and weight of 1,000 seeds was 51.78 ± 0.021 ㎎. Thus the seed was classified as ‘dwarf seeds’. As the result of dormancy characteristics, moisture content of freshly matured seeds increased rapidly by water-soaking treatment and seeds did not germinate at 20℃ for 4 weeks under the light condition. Therefore, seeds were estimated to have physiological dormancy. Germination conditions of dry-stored seeds were found to be 20℃ and light condition, and seeds did not germinated under dark condition regardless of all temperature regimes. Therefore, Alpine modest primrose seed was considered as photoblastic type. Percent germination (PG) and germination energy (GE) were greatly improved by soaking the seeds in GA3 and kinetin for 24 hours. Especially, 200~500 ㎎/L GA3 treatment resulted in the highest PG (95.5%) and GE (98.3%). So, chemical treatment such as GA3 was thought to be a useful method for raising seedling uniformly.

This study aimed to investigate the morphological characteristics and optimal germination conditions in seeds of Arabis pendula L., a traditionally edible and medicinal plant. The external seed shape was circular-obovate with narrow wings and dark brown. The seed length and width were 1.25 ㎜ and 1.47 ㎜, respectively. The seeds were exalbuminous and the embryo was a bent type. Seed germination was the highest (49.7%) at 20°C under dark conditions among the various temperature and light conditions applied. However, under the dark condition, the seedling was weak, overgrown, and the cotyledons were small and folded. To improve the germination and growth of seedlings, the seeds were pre-treated with GA3 solutions of varying concentrations (0, 200, 500, and 1,000 ㎎/L). The seed germination and seedling growth were effectively improved by GA3 pre-treatment. The germination rate was the highest (97.3%), mean germination time was the shortest (8.1 days), and a vigorous growth of seedlings was observed upon pre-soaking the seeds in 500 ㎎/L GA3 solution. In conclusion, the best method for germination was pre-soaking in 500 ㎎/L GA3 (4℃, dark, 24 h) and incubating the seeds at 20℃ for 15 days.

This research was performed to develop mass propagation method of round-leaved sundew (Drosera rotundifolia L.) for improving horticultural usefulness. Seeds were collected around Hwaseong-si, Gyeonggi-do in October of 2011 and experimented upon while being dry-stored at 4 ± 1.0℃ (darkness). Seed length of a major and minor axis were 1.58 ± 0.060 and 0.21 ± 0.016 ㎜, respecrively, and weight of 1,000 seeds was 6.24 ± 0.172 ㎎. Seeds were thus classified as ‘dwarf seeds’. Regarding the dormancy type, since round-leaved sundew seeds were dormancy broken and germinated at 20~30℃ under the light condition after wet-chilling treatment for 12 weeks, seeds were estimated to have physiological dormancy. Germination conditions of dormancy broken seeds were found to be 20℃ and light condition (54.7%), but germination decreased at higher temperature. Percent germination (PG), germination energy (GE), mean germination time (MGT) and T50 were effectively improved by chemical treatment such as GA3 200 ㎎/L + kinetin 20 ㎎/L and wet-chilling treatment for 14 weeks. In conclusion these optimal conditions were thought to be a useful method for raising seedling uniformly.

Background : The study was conducted in response to climate change, to develop cultivation techniques by introducing a new crop income. Therefore, we want to collect the Coriander sativum genetic resources that are grown in different regions of the korea and other countries. The C. sativum were strengthens the stomach to say "housil" in Herbal and prosper digestion. Recently, several nations, including Vietnam and Thail and increased in Korea. C. sativum increased eating enjoy food. The people who like C. sativum in Korea is estimated to be 1.5 million. Methods and Result : First was C. sativum 93 species in National agrobiodiversity Center RDA lotting-out on May 12, 2016 for the study. C. sativum germplasm received lotting-out of over thousand grain 15g 28 kinds of seeds, 10∼15g was 23 species, 10g less than 42 species. 32 kinds of fruit color is white, black, 30 species, the gray was 31 species. Conclusion : C. sativum lotting-out was conducted by resource for two weeks from germination test from May 31 to June 13. The results were as follows: C. sativum port germination 86∼100% germination was 43 stocks, 40∼60% of 23 stocks, 5 stocks of 20∼ 40%. The 22 stocks were not germinate paper. C. sativum resources per port germination test was conducted 13 days from 29 days July until August 9th. As a result, more than 90% of the port germination is 62 stocks, not more than 80∼90%, 50∼80% 18 stocks of germination was 5 stocks. At this time, no germination was 8 stocks. Therefore, C. sativum germination test is a port germination test obtained a more than satisfactory result.

This research was carried out to investigate the germination and seedling growth characteristics of the halophyte Suaeda asparagoides under various salt concentrations, temperatures, and the presence or absence of light S. asparagoides has a non-endospermic dicotyledonous seed structure that is go stone-shaped with a dark brown color and covered by a star-shaped perianth. Germination within the temperature range 5~40°C varied from 8% to 65%. The highest germination occurred at 15°C. In seeds exposed to salt concentrations in the range 0~400 mM, the highest germination occurred at 0 mM, and the germination progressively decreased as the salt concentration increased. When the perianth of the seed was removed, the germination increased significantly. In the presence or absence of light, germination under white light was 5~20% higher than that under darkness, which varied according to salt concentrations in the range 0~300 mM. The plant growth of S. asparagoides, evaluated by plant height, branch number, root length, fresh weight, and dry weight, was higher in the concentration range 50~100 mM, and it decreased in the other concentrations. In conclusion, the seeds of S. asparagoides showed higher germination in fresh water at low temperature of approximately 15°C under light when the perianth was removed, and the plant growth was higher at salt concentrations of 50~100 mM.

Background: Many Taraxacum spp. plants are widely used in medicine, but some of them have propagation problems, such as strong dormancy and poor germination rates. This study investigated the effects of temperature, gibberellic acid (GA3), and potassium nitrate (KNO3) on seed germination in Taraxacum ohwianum. Methods and Results: The seeds (NIBRGR0000135524) were exalbuminous, and their length and width were 4.54 ± 0.032㎜ and 0.97 ± 0.029㎜, respectively. Various germination temperatures were tested (15, 20, 25, and 30℃), the optimum temperature for germination was found to be 20℃ (31.3%). High temperature (30℃) resulted in non-typical seedlings (thickened and crumpled cotyledons, with a restricted root system). To increase the germination capacity, GA3 (200, 500, and 1,000 ㎎/ℓ ) or KNO3 (20, 50, 100, 200, and 500 ㎎/ℓ ) solutions were used as pre-soaking solutions instead of water. The GA3 treatments increased the germination percentage and rate, but germination percentage was higher with the KNO3 treatments. Under the 50 - 200 ㎎/ℓ KNO3 treatments, the germination percentage exceeded 80% after 12 days, and 50 ㎎/ℓ KNO3 was notably effective (91.2% after 15 days). Conclusions: T. ohwianum seeds showed improved germination at low temperatures. The best method for germination was presoaking in 50 ㎎/ℓ KNO3 for 24 h, in the dark at 4℃, and then incubating the germinated seed at 20℃ for 15 days.

Background : Plants of Taraxacum spp. are widely used in medicine, but some of them have some problems related to propagation, such as strong dormancy and inactive germination. This study investigated the effects of temperature, gibberellic acid (GA3), and potassium nitrate (KNO3) on seed germination in Taraxacum ohwianum. Methods and Results : The seeds (NIBRGR0000135524) were exalbuminous, and their length and width were 4.25 ± 0.118 mm and 0.89 ± 0.062 mm, respectively. Among various temperatures (15, 20, 25, and 30℃), the optimum temperature for germination was found to be 20℃ (31.3%). High temperature (30℃) induced off-type in seedlings (thickened and crumpled cotyledons, and restricted root system). GA3 treatments increased germination percentage and speed, but germination percentage was higher with KNO3 treatment. Under KNO3 treatments (50 to 200 mg·L-1), germination percentage exceeded 80% after 12 days, with 50 mg·L-1 KNO3 being notably effective (91.2%, after 15 days). Conclusion : Seeds of T. ohwianum showed germination ability at low temperature. The best method for germination was pre-soaking in 50 mg·L-1 KNO3 (4℃, dark, 24 h) and incubating at 20℃ for 15 days.

Background: The purpose of this study was to evaluate methods to reduce seeding expenses, thereby increasing farm income. This study investigated the effects of rain shelter controlled cultivation and adapted seeding times on the stable seed production of Scutellaria baicalensis Georgi. Methods and Results: Seed germination was conducted under 10 condition compose to control, water washing, cold storage at 4℃ for 15 days, seed sterilization with a benomyl pesticides, hormone treated seed by submerging in 100 ppm GA3 with the cold storage at 20℃ and 25℃, darkness in a covered petridish and illuminated with a 1,500 Lux lamp. There were three cultivation type, open cultivation with non-woven fabric mulching, cultivation with a vinyl covering and rain sheltered in a plastic greenhouse. Sowing dates were April 27, May 18, June 7 and June 28, 2013. Plants were spaced 10 ㎝ apart in rows 30 ㎝ apart. Mixed oil cake fertilizer, N-P2O5-K2O (12-10-10) was applied at 600 ㎏• 10 a−1. Conclusions: Optimum germination occured in darkness at 25℃ and cold storage after submerging in GA3. The highest seed yields (4.5 ㎏• 10 a−1) occurred in the plastic greenhouse for the April 25 sowing. The highest root yield (17%) was found on April 1, under greenhouse conditions.

Background : Acer pictum is a deciduous tree belonging to the family Aceraceae. This study was conducted to collect basic data on mass propagation techniques by surveying the germination and growth characteristics of A. pictum seeds. Methods and Results : A. pictum seeds were harvested in 2014 on August 19, September 5, September 29 and October 31. The seeds were then sown on the same dates they were harvested. A portion of seeds harvested in August 2014 were stored at low temperatures and subsequently sown on March 11, 2015. The germination rate of A. pictum seeds was highest in seeds that were stored harvested on October 31. Mean germination time (MGT) was shortest and germination velocity (Rs) was fastest in seeds harvested on October 31. Root collar diameter, total root length, and dry weight were also highest from seeds collected October 31. Conclusions : A. pictum seeds harvested on October 31 had highest germination and growth rates compared with seeds harvested on earlier dates. It is expected that these results demonstrating optimum harvesting and sowing dates will be applicable to future seedling production for this tree species.

This study was conducted to obtain the basic data related to seed characteristics and germination conditions of the Coriandrum sativum L. at different storage condition and temperature. The shape of fruit was oval with light brown color. Fruit was mericarp, biloculate, with one ovule in each locus. The length and width of seed were 1.37 ± 0.067㎜ and 0.52 ± 0.039㎜, respectively. Weight of 1,000 seeds was 6.55 ± 0.15 g. Seeds which were stored at room (15℃) and cold temperature (4℃) in vinyl container showed the highest germination rate (93.3%) under the room temperature germination condition. Percentage of germination in cold and freeze temperature was 20% and 0%, respectively. Germination rate of room storage seeds with paper container increased to about 91.3%, however, decreased in cold (4℃) and freeze storage (−20℃) with paper container (76.7% and 78.0%, respectively). Germination rate of seed in vinyl stock container was better than that of paper stock container. Germination rate of seeds stored at room temperature for 4 years (2010 - 2013) ranged from 80.0% to 91.3%. Therefore, coriander seeds are expected to be stored at room temperature for increasing the germination rate and keeping a long term.

Senna tora (L.) Roxb. belongs to Leguminosae and its seeds are usually roasted and boiled in water to produce tea in Korea. Also the plants are well known for the treatment of Hypertension, Hepatitis, Constipation and Conjunctivitis. This study was conducted to investigate the morphological characteristics and pollen germination rates of Senna tora with the aim of genetic mapping of this species. First, we investigated morphological characteristics of domestic and international genotypes containing 51 lines in Korea and 2 lines in China. No significant differences in growth characteristics were observed among 53 genotypes. However, ST-9 line which was collected in Pyung-chang showed high growth rate at the early stage. The flower of Senna tora consists of 5 petals, 10 surgeries (7 main surgeries, 3 small surgeries), 1 pistil, and 5 sepals. After bud emergence, each petal was white in 1-2 days, turn into ivory in a week, then yellow in 8-9 days and finally bloomed in 9-10 days. Although the average flowering times of the plants were July 24, ST9 flowered in July 12. In addition, the flowers of ST9 differed from the flowers of the other genotypes. Flower of ST9 joined together with one another. Therefore, ST9 showing high growth rate at the early stage and unique flowering characteristics was selected to as the paternal line for genetic mapping study. Second, we investigated pollen germination rates for each stage of flower development. Pollen started to germinate at the yellow bud stage and pollen germination rate was highest in the bloomed flower stage. This results show that self-fertilization hardly occurs when the flower is ivory bud stage, and there is no need to use young bud flowers for artificial crossing. This work is intended to serve as the basis for the breeding of new varieties in Senna tora.

This study was conducted to improve the postharvest storage techniques of managing and storing seeds, totest qualities and viabilities of the seeds and to examine the germination rate and the protein expression of Achyranthesjaponica Nakai. The seeds collected from different areas of Je-Cheon and Gwang-Ju were stored with different temperaturesand durations. Two plant growth regulators and two seed priming were treated to investigate their effect on the germinationrates and the days required for germination. The weight of one hundred seed collected in Gwang-Ju was heavier than thosein Je-Cheon. Seed length collected in Gwang-Ju was also longer about 5.12㎜ than those in Je-Cheon about 4.90㎜ andseed width was longer in Gwang-Ju than those in Je-Cheon. The rates of seed germination in two different collection areaswere higher about 2.9 to 13.0% in Gwang-Ju compared to those in Je-Cheon. Comparing its rates with the storing tempera-tures and durations, they were not clearly different in between 4℃ and 25℃ and they also were gradually decreased withgetting longer storing durations. The germination rates treated by plant growth regulators were higher with GA3 than thosewith Kinetin. The highest seed germination rate was appeared at 50 ppm of GA3. Comparing its rates with different seedpriming, they were relatively higher with KNO3 than those with PEG6000. In protein expression patterns between before thegerminating and after the germinating of seeds, more and clear bands were appeared in the seed after the germination com-pared to those before the germination of seeds, especially 10~20kDa. These results showing more and clear bands weremore clearly appeared in Gwang-Ju compared to Je-Cheon. Comparing the protein expression with plant growth regulatorsand seed primings, GA3 was better expression than those with Kinetin and KNO3 was better than those with PEG6000. Moreand clear bands were closely related to the germination rates of seeds and more detailed studies would be required.

Characteristic of seed for Sageretia thea fruits collected from the habitat of harvest season in Jeju and theirgermination ratio in different temperatures were considered. The average weight was 0.2g, average diameter was 7.2㎜,average length was 6.5㎜, and size distribution range was 5.1~10.0mm. The number of seeds per fruit was 1.8, and 1000grain weight was 7.77g with diameter of 3.7㎜ and thickness of 1.7㎜ in size. The fruit maturation was investigated to befrom April 27 to June 1, when the best maturation period was about a week, May 11 to May 18. Also, the fruits grown onMay 4 to May 11 were seen to have the bigger in transverse size and weight with a tendency of the earlier maturation datehas the greater the number of seeds. S. thea seeds pretreated for 24 hours at 50℃ were not germinated, while most of thosepretreated for 24 hours at 4℃ were successfully germinated at any degree of temperature (except at a temperature of 10℃).Particularly, the highest germination rate of 55% was made at 15℃, and plumule and radicle were best grown within thetemperature range of 25℃.

This study was conducted to investigate the quality of seeds, the germination rates and the days required for germination, to examine the patterns of protein expressions during the germination and to improve the techniques of managing and storing seeds and viability of the seeds of Lithospermum erythrorhizon Sieb. et Zucc. After collecting and harvesting seeds, they were classified to white and brown colors of seed coat through testing their seed size, weight, and quality. The germination rates, the days required for germination, and the protein expressions were examined with different colors of seed coats, storing temperatures and durations by treating the different plant growth regulators and primings. One hundred seed weight of white color was heavier about 1.17 g than those of brown one about 0.81 g. The germination rates in white color of seed coat was higher, 3.05 ~ 5.75%, than those in brown one. Its rates were decreased with getting longer in storage durations. There was no big differences on germination rates between storage temperatures. The plant growth regulator of GA3 and Kinetin was affected to improve the seed germination. GA3 increased the seed germination clearly at 25 ppm level, while kinetin increased it gradually from 25 to 100 ppm levels. In germination by seed primings, PEG6000 made higher germination rate with increasing their levels, whereas KNO3 increased the germination until 100 mM level and then decreased it with 200 mM unlike PEG6000. The protein expressed during the seed germination were appeared more and clearer bands in the seed after germination, especially 20 ~ 30 kDa, compared to those in the seed before germination. These results showing more and clearer bands were positively related to the germination rates which were different by seed colors, storage temperatures and durations, and plant growth regulators and primings.