This study improved the work efficiency by supplementing the shortcomings of the manual process by developing a double tube feeding device, and the following results were obtained by conducting the production capacity, production length, and defect rate tests. Developed a double tube production system to enable the simultaneous production of two tubes, increasing the production volume by about 1.5 times. The product length has been improved from semi-automatic to automated, and the production capacity has been improved from 16 to 25 pieces per hour (based on 15m). Developed a double-tube input straight line automatic adjustment feeder, which resulted in reducing the defect rate to less than 1%.

This paper develops an algorithm to determine the batch size of the batch process in real time for improving production and efficient control of production system with multiple processes and batch processes. It is so important to find the batch size of the batch process, because the variability arising from the batch process in the production system affects the capacity of the production. Specifically, batch size could change system efficiency such as throughput, WIP (Work In Process) in production system, batch formation time and so on. In order to improve the system variability and productivity, real time batch size determined by considering the preparation time and batch formation time according to the number of operation of the batch process. The purpose of the study is to control the WIP by applying CONWIP production system method in the production line and implements an algorithm for a real time batch size decision in a batch process that requires long work preparation time and affects system efficiency. In order to verify the efficiency of the developed algorithm that determine the batch size in a real time, an existed production system with fixed the batch size will be implemented first and determines that batch size in real time considering WIP in queue and average lead time in the current system. To comparing the efficiency of a system with a fixed batch size and a system that determines a batch size in real time, the results are analyzed using three evaluation indexes of lead time, throughput, and average WIP of the queue.

The automobile manufacture is a typical integrated assembly production that the numerous parts are committed sequentially to the assembly line. In this type of automobile manufacture, WBS (white body storage) and PBS (paint body storage) are operated to adjust the production sequence and to buffer. Starvation (impossibility of production owing to the insufficient supply) or blocking (impossibility of production owing to the over capacity) could occur in some chance, hence, both storages (WBS, PBS) are integrated as a prevention. This system is called integrated storage. In this paper, we suggest the simulation model of ‘WBS-PBS integrated storage’ and methodology of operation considering both ‘Color grouping’ and ‘Option leveling.’

Urokinas type plasminogen activator (uPA) has been used as a therapeutic agent for treating human diseases such as thrombosis. Attempts to transgenically overexpress the uPA in animal bioreactors have been hampered due to side effects associated with this functional protein hormone on homeostasis. Recently, chicken has been emerged as a potential candidate for use as bioreactor to produce proteins of pharmaceutical importance. Since this species has low homology uPA sequence with mammals, we hypothesized that chicken could be used as a potential bioreactor for production of human uPA. In this study, using replication‐defective Murine Leukemia Virus (MLV)‐based retrovirus vectors encapsidated with Vesicular Stomatitis Virus G Glycoprotein (VSV‐G), we attempted to make transgenic chicken expressing human uPA (huPA). The recombinant retrovirus was injected beneath the blastoderm of non‐incubated chicken embryos (stage X, at laying). After 21 days of incubation (at hatching), all of the 38 living chicks that assayed, were found to express the vector‐encoded huPA gene in various organs and tissues, which was under the control of the Rous Sarcoma Virus (RSV) or Cytomegalovirus (CMV) promoter. Using specific primer set for huPA, PCR and RTPCR analyses of gDNA isolated from these samples demonstrated these chickens were transgenic for huPA. Furthermore, successful germ line transmission of huPA transgene was confirmed and next generation whole body huPA transgenic chickens were also produced. We also assayed huPA protein titer in blood (17.1 IU/ml) and eggs (4.4 IU/ml) of whole body huPA transgenic chicken. Thus, our results demonstrated that chicken could be used as bioreactors to produce huPA.

This study was various sensitive to a customer requirement and sensitive to the market environment of cosmetics with the manufacture company the low. Consequently we arrange the making line properly and are there even though we make a productivity enhance with the personnel expenses reduction to the quality enhance. We do the supplementation with existing U-line the defect of Cell-line of the making method. We try we substitute and to present the improvement direction to apply at the characteristic of the making company so that we are soft.

본 연구는 재래 돼지의 산육 능력을 개량하기 위한 기초 자료를 제공하기 위하여 축산과학원에서 2001년부터 2006년까지 20 kg에 검정을 개시하고 70 kg에 검정을 종료하여 발육 능력을 조사한 546개의 재래 돼지 산육 능력 검정 자료를 활용하여 유전모수를 추정하였으며, 재래 돼지 사육 농가의 사양방법 개선을 위하여 2003년부터 2005년까지 조사된 재래 돼지성돈 및 검정돈 132두의 발육 단계별 체중 및 체위 조사 자료를 다중 회귀 분석하여 재

New product line with additional secondary operation of oil pump rotor was built in necessity of increasing capacity. This new line includes steam treatment process after sizing. The new line achieved 1.6 times higher performance compared with a conventional line.

The development potential of bovine somatic cells was evaluated using nuclear transfer. A single donor cell derived from fetus of HanWoo(Korean Native Cattle) was selected and deposited into perivitelline space of each enucleated oocyte before electrical fusion and activation. Nuclei of donor cells starved for 7 days (37%) tended to support the development of reconstitute embryo the blastocyst stage better than those of donor cells starved 3, 14 and 30 days. The cleavage rate was significantly lower(P<0.05) in reconstitute embryos derived from large size donor cells(51.2%), than those from small medium size donor cells(76.6 and 73.5, respectively). The developmental rate to blastocyst of reconstructed embryos from medium size donor cells was higher than those from small and medium size donor cells. This study demonstrates that an appropriate culture period for induction into quiescent stage and the size of donor cells effect on the efficiency of nuclear transfer using cultured bovine cells.

1987년 6월-1988년 4월까지 격월로 제주도 해안선주변 10개 정점에서 부유성 갑각류의 현존량과 생산량을 야간채집, 조사하였다. 계절별 평균개체수는 12월에 가장 낮고, 6월에 가장 높았는데, 이들 개체의 대부분(90% 이상)은 90μm 망목의 네트로 채집된 것으로 이뤄졌다. 계절별 평균 현존량의 범위는 6.6-55.0mgm^(-1)(평균 16mgm^(-3))이고, 여름에 높았다. 일일평균 생산량 역시 여름(369μgCm^(-3)d(-1))에 높고 겨울(112μgCm^(-1)d^(-1))에 낮았다. P/B의 비(평균 0.25)는, 수온이 높고 소형 동물플랑크톤의 개체수가 많은 계절에 높은 값을 나타냈다.

The primary production at 10 stations around the coast line of Cheju Island has been investigated from the viewpoint of standing crops(cells/e), chlorophyll-a concentration and CO₂ fixation of phytoplankton during the period from June, 1987 to March, 1989. Phytoplankton standing crops ranged from 2,025 to133,734 cells/ethrough the year, increasing from June and showing a peak in August. The distribution of standing crops was the highest in the eastern area and the lowest in the northern area. Chlorophyll-a concentration ranged from 0.45 to 2.59 ㎎/㎥ during the study period, which was much lower than that in the other Korean coastal waters. To estimate the CO₂ fixation of phytoplankton, daily primary production (㎎C/㎥/day) has been determined using C-14 method. It ranged 1.31-7.15, 34.54117.04, 22.283.64, 8.53-11.95㎎㎎/㎥/㎥/day in winter, summer, fall, and spring, respectively, and showed the highest in the western area. The carbon assimilation number(㎎C/㎎ chl-a/hr)was also higher in summer than in other seasons. The general characteristics of primary production of phytoplankton around the coast line of Cheju Island was high in the eastern and western regions during the summer season.

Male sterile line in onion is essential to the development of hybrid varieties. Onion male sterile line 'Wonye30005' was developed by Bioenergy Crop Research Center, NICS, RDA in South Korea in 2010. Lodging date of 'Wonye30005' is May 15th and it belongs to the early maturing type in Korea. We found the male sterile plants in ‘Hamasodachi’, early maturing open-pollinated variety, and the male-sterile plants were cross-pollinated with male fertile line, ‘M330’, the population of F1 showed 100% male sterility and the F1 plants were subsequently backcrossed for five generations. 'Wonye30005' was numbered as ‘MMS77’ in 2009 and the corresponding male parent is called MMSB77. 'Wonye30005' has round bulb shape (Bulb index, 97), cold resistance, and excellent storage quality and average bulb weight is 253g. Flowering date is May 25th and flower bud size is 84 mm in full bloom. The number and length of flower stalks per bulb are 4 and 134 cm, respectively. 'Wonye30005' has been filed in Korea Seed and Variety Service in 2010. Overall, 'Wonye30005' is promising male sterile line for early maturing type hybrid seed production and breeding program. (no 4673)

양파 웅성불임 계통 ‘원예30006’은 2000년에 중생계통인 ‘F2-6’집단에서 웅성불임개체를 선발하여 자식계통인 ‘HMB’를 화분친으로 하여 교배하였다. 2002년에 유지계통을 선발하고 2010년까지 5회 여교잡을 하여 계통의 안정성을 확인한 후 ‘MMS66’으로 명명하고 2010년에 직무육성신품종 심사를 거쳐 2011년에 품종보호출원 하였다. ‘원예30006’의 초형은 직립이며 중생종으로 도복기는 5월 25일, 초장 56 cm, 엽수 8매, 엽초경 12.4 mm로 초세가 중간정도이며 엽초경이 가늘다. 구중은 184 g의 중구형에 속하며, 구형지수 99로 원형이다. 저장성과 내한성이 강하고 노균병에 다소 저항성이 있다. 채종모구의 개화기는 6월 4일, 화경수 4개, 화경장 115 cm, 화구폭 86 mm이며 웅성불임 발현이 안정적이다.

To search for immunoactive natural products exerting anti-inflammatory activity, we have evaluated the effects of the ethanol extracts of Rubus coreanus Miq. (ERC) on lipopolysaccharide-induced nitric oxide (NO), tumor necrosis factor-α (TNF-α), and Interferon-γ (IFN-γ) production by RAW 264.7 macrophage cell line. Our data indicate that this extract is a potent inhibitor of NO production and it also significantly decreased IFN-γ and TNF-α production. Consistent with these results, the protein level of inducible Nitric Oxide Synthase (iNOS) and cyclooxygenase-2 (COX-2) was inhibited by ethanol extracts of ERC in a dose-dependent manner. These results suggest that ERC may exert anti-inflammatory and analgesic effects possibly by suppressing the inducible NO synthase and COX-2 expressions.

수입에 의 한 수요충족이 절대불가능한 양질다수성 싸이레지용 옥수수의 개발은 우리나라에 매우 필요한 사항이다. 양질 다수성인 싸이레지용 옥수수의 개발을 위한 육종적 재료를 확보코저 재래종 옥수수의 수집종 가운데서 선발된 다약다수성인 계통을 가지고 재배환경이 다양한 조건하에서 싸이레지용으로서의 가부시험을 한 결과는 다음과 같이 요약된다. 1. 싸이레지용 주요특성의 하나인 단위면적당 총건물중 생산양에 있어서 파종기나 재배밀도에 관계없이 수원 19호 교잡종보다 다약다종성인 MET 계통이 평균 10% 더 높았다. 2. 식물체의 부입별 특성을 비교하면 MET 계통은 건엽중이 수원 19호보다 월등히 높아 MET 계통의 건물중 증가에 기여하였다. 3. MET 계통의 건엽중이 수원 19호보다 높았던 것은 MET계통의 분벽에 의한 엽수의 증가 때문이었으며, 4. 기타 곡실수량에 관련된 이삭의 무게라든가 입중, 이확속중 등은 수원 19호가 높았다. 다만 포엽중만은 MET 계통이 다수성으로 인해 더 높았다. 5. TDN의 증감과 관계가 있는 100a당 배의 생산은 소입성인 MET 계통이 수원 19호보다 10~ 15% 더 높았다. 6. MET 계통이 다약다수성이고 수원 19호가 무약일수성인데도 불구하고 분산분석결과 품종 재배환경(파종기 또는 재식밀도)라는 유의적인 상호작용이 없이 다 같이 밀식함으로써 총건물중이 증가하였다. 7. 싸이레지로 만들었을 때 MET 계통과 수원 19호의 일반조성분 TDN 등을 비교한 결과 일반 조성분에 있어서는 두 품종간에 큰 차이가 없었으나 TDN에 있어서는 MET 계통이 더 높았고 DCP에 있어서는 수원 19호가 더 높았다. 8. 아미노산의 분석결과 두 품종간에 큰 차이는 없었다.60cm)한 경우 밀식(60 20cm)한 경우와 같이 분벽들에 의한 밀식의 효과가 있었고 중간재식밀도(60 40cm)인 경우에는 소ㆍ밀도의 중간되는 기장을 보였다. 4. MET계통은 개체당 이삭수가 많은 것에 반하여 이삭의 크기는 수원 19호의 13도 못되었다. 각분벽기에 달리는 이삭수는 소식의 경우 주기에 4개, 제일, 이분벽기에 2～3개씩 달렸다. MET계통의 입은 경입종이며 황색입이 주가되고 백색입이 약간 섞여 있었으며 100입중은 14~15gr. 의 소입종이었다. MET계통은 착수고가 수원 19호보다 훨씬 높았다. 5. 주요 특성간의 상간 : 수원 19호에 비하여 MET 계통은 주요 특성간에 높은 상관계수를 보여 유전상관도 높을 것이라는 것을 간접적으로 나타내주었다. 6. MET계통은 생장속도가 수원 19호보다 훨씬 늦었으며 개화기도 2~3주나 늦었다. 7. 흑조위축병 : 수원 19호와 같이 MET계통도 흑조위축병에 대해 이병성이었다. 이병성은 늦게 파종하거나 밀식할수록 높아지는 경향이 있었다..7g로서 가장 무거워 많은 차이를 보이었다. 10a당 정조중은 대조구에 비하여 Dolomite처리구가 8.6%, 유황첨가구가 5.7~7.4% 증수되었음을 보이었다. 7. 식물체중의 조단백질 함량은 대조구, 5%, 10% 유황첨가구가 3.31~3.50%로서 비슷하였고 15% 유황첨가구는 3.94%, Dolomite첨가구는 5.38%였다. 아미노산도 15%유황첨가구와 Dolomite첨가구가 많이 함유되어 있었다. 현미중 조단백질은 15%유황첨가구가 10.14%로서 최고이었으며 10%유황첨가구와 Dolomite첨가구는 9.85%로서 다음이었다. 아미노산도 대조구의