Background: The biological information of fish, which include reproduction, is the prerequisite and the basis for the assessment of fisheries. Methods: The aim of this work was to know the reproductive biology with the first sexual maturity (TL50) and the spawning period for 58 mainly fish species in the waters around La Réunion Island (Western Indian Ocean). Twenty families belonging to the Actinopterygii were represented (acanthuridae, berycidae, bramidae, carangidae, cirrhitidae, gempylidae, holocentridae, kyphosidae, labridae, lethrinidae, lutjanidae, malacanthidae, monacanthidae, mullidae, polymixiidae, pomacentridae, scaridae, scorpaenidae, serranidae, sparidae; 56 species; n = 9,751) and two families belonging to the Elasmobranchii (squalidae, centrophoridae; 2 species; n = 781) were sampled. Between 2014 and 2022, 10,532 individuals were sampled covering the maximum months number to follow the reproduction periods of these species. Results: TL50 for the males and the females, respectively, ranged from 103.9 cm (Acanthurus triostegus ) to 1,119.3 cm (Thyrsitoides marleyi ) and from 111.7 cm (A. triostegus ) to 613.1 cm (Centrophorus moluccensis ). The reproduction period could be very different between the species from the very tight peak to a large peak covered all months. Conclusions: Most species breed between October and March but it was not the trend for all species around La Réunion Island.

Bolivian tuber species like potato (Solanum tuberosum), native potato (Solanum sp), Oca (Oxalis tuberosa Molina), Olluco (Ullucus tuberosus Caldas), and Isaño (Tropaeolum turosum Ruíz & Pav.) hold extraordinary nutritional value and cultural significance, particularly within the Andean region. This study examined the mineral composition of Bolivian tuber species as an essential step toward understanding their nutritional significance and potential contributions to addressing dietary deficiencies. The research involved detailed analysis of diverse tuber cultivars, uncovering distinct mineral profiles across species. Native potato shows high levels of nitrogen (N), potassium (K), phosphorus (P), and magnesium (Mg) levels, alongside moderate micronutrients like iron (Fe) and zinc (Zn). Commercial potatoes exhibited prominence in N, P, and K, with moderate Fe, Zn, and manganese (Mn) levels. Oca, Isaño, and Papa Lisa displayed unique mineral concentrations, offering potential nutritional benefits. Intricate correlations and significant variances among elements highlighted the diverse mineral compositions among these tuber species. Multivariate analyses emphasized distinct mineral profiles unique to each species, revealing significant compositions of isaño and papa lisa's. The Multitrait Genotype- Ideotype Distance Index (MGIDI) identified isaño jaspeado, isaño and an unnamed native potato, AXT2, as promising ideotypes due to their exceptional mineral compositions. These findings provide comprehensive insights into Bolivian tuber species' various mineral compositions, underscoring their nutritional significance and potential in targeted breeding for improved dietary support and enhanced food security.

The ecosystem provides a diverse array of environmental conditions for organisms, and only those that are capable of successfully adapting to these conditions within their habitats can endure, thrive, and proliferate. Further, the environmental conditions within these habitats can significantly affect the bioavailability of chemicals that are introduced therein, thus resulting in varied adverse impacts on the organisms. The present study aims to evaluate the sensitivity of Yuukianura szeptyckii - a species adapted to riparian - to heavy metals following ISO guideline 11276, with the objective of assessing its potential as an indicator species for ecotoxicological evaluations in riparian habitats. The findings revealed that cadmium and copper both had significant toxic effects depending on their concentrations. For cadmium, the LC50 was 280 mg kg-1, EC50 was 66 mg kg-1, and NOEC and LOEC were 25 and 50 mg kg-1, respectively. For copper, the LC50 was 911 mg kg-1, EC50 was 151 mg kg-1, and LOEC was 50 mg kg-1. Comparative analysis with previous results for the international standard species Folsomia candida and the domestic standard species Allonychiurus kimi indicated that Y. szeptyckii exhibited even greater sensitivity to toxicity values. The adverse effects on survival and reproduction were closely associated with the influx concentration of heavy metals in their bodies. Altogether, the results suggest that Y. szeptyckii is a sensitive species for ecotoxicological assessments in riparian habitats, thus making it suitable as an indicator species, particularly in riparian ecosystems that are characterized by relatively high humidity conditions.

꽃노랑총채벌레(Frankliniella occidentalis)와 오이총채벌레(Thrips palmi)는 경제적으로 오이의 중요한 해충이며 이들의 방제를 위해 사용 되는 화학약제는 효과적일 수 있으나 생식용으로 소비되는 오이에서 자주 사용하는 것은 바람직하지 않다. 따라서, 이들의 방제를 위해 화학농약 을 대체할 수 있는 천적인 미끌애꽃노린재(Orius laevigatus)의 방제효율을 증대시킬 수 있는 방법으로 미끌애꽃노린재의 천적유지식물을 선발하 였다. 실험실 조건에서 4가지 식물종에 대한 미끌애꽃노린재 부화율, 출현율, 생존률, 생식력을 조사하였고 오이 재배시설 내에서 선발된 2종의 천적유지식물에 대하여 미끌애꽃노린재 밀도를 조사하였다. 미끌애꽃노린재 알 부화율은 채송화가 92%로 가장 높았고 출현율은 바질이 81.4% 로 가장 높았으며, 생존률은 채송화와 바질이 각각 70.8%와 69.1%로 통계적 차이를 보이지 않았다. 미끌애꽃노린재 생식력과 산란기간은 모두 채송화가 각각 209.8개와 20.2일로 가장 좋았고 바질은 각각 160.2개와 15.8일이었다. 식물체 꽃의 개화시기는 화밀을 대체먹이로 하는 미끌애 꽃노린재의 생존에 중요한 요소이기 때문에 채송화와 바질의 개화시기를 조사한 결과 채송화는 7월에서 10월 개화하는 반면 바질은 4월부터 9월 까지 개화하였다. 시설 오이에 미끌애꽃노린재의 천적유지식물로 바질과 채송화를 투입하고 미끌애꽃노린재 밀도를 조사한 결과 바질이 투입된 곳에서 밀도가 높게 조사된 반면 채송화를 투입한 곳에서는 거의 보이지 않았다. 따라서, 바질은 미끌애꽃노린재의 천적유지식물로서 잠재적 가 치가 가장 높았으며 총채벌레 방제를 위해 미끌애꽃노린재를 이용할 경우 바질을 천적유지식물로 동시에 사용한다면 미끌애꽃노린재의 생물적 방제효과에 대한 효력을 증대시킬 수 있을 것으로 기대된다.

The chemical composition of 86 species of native plants in Korea, including plants to be afforestation, was analyzed. The chemical composition of the species analyzed was different. The species with the highest extractable content was Viburnum dilatatum (3.91%), and the species with the lowest extractable content was Ligustrum lucidum (0.11%). The lignin content ranged from 12 to 39%, with an average of 25%. The species with the highest lignin content was Chaenomeles lagenaria (39.37%). Hemicellulose content ranged from 18 to 52%, with the highest species being Thuja occidentalis (51.22%) and Eucommia ulmoides (48.84%). Cellulose content ranged from 25 to 58%, and the species with the highest content were Prunus serrulata (57.67%), Diospyros kaki (57.14%), Aesculus turbinata (53.29%), Albizia julibrissin (53.02%), and Zelkova serrata (52.29%). The chemical composition was different for each use taxon of 86 plant species. The lignin content was the highest in the fruit group and the lowest in the group other than recommended species for afforestation. Cellulose content was highest in non-reforestation-recommended tree species and lowest in fruit trees. In classification according to tree height, lignin content was higher in shrubs than in tall trees, and cellulose content was highest in tall trees. Between deciduous and evergreen trees, the lignin content was high in deciduous trees (26.46%), and the cellulose content was also high in deciduous trees (44.01%). As a result of analyzing the correlation between each compound, there was a difference. There tended to be a positive correlation between extractives and lignin content. There was a negative correlation between extractives and holocellulose content, hemicellulose and cellulose. The higher extract content affected the cellulose content much more than hemicellulose. Also, the higher the lignin content, the lower the cellulose content. The species with low lignin content and high cellulose content were Diospyros kaki and Prunus serrulata var. spontanea. This result is expected to be primary data for bioenergy, pulp industry and bioindustry.

벽계천의 어류군집 특성 및 멸종위기 4종의 서식 양상을 밝히기 위해 2020년 4월부터 9월까지 조사를 실시하였다. 조사기간 동안 7개 지점을 선정하여 조사한 결과, 채집된 어류는 9과 31종 3,415개체였다. 출현종 중 우점종은 참갈겨니 (Zacco koreanus, 31.2%), 아우점종은 피라미(Z. platypus, 15.0%), 다음으로 돌고기(Pungtungia herzi, 11.7%), 줄납자 루(Acheilognathus yamatsutae, 5.4%), 납자루(A. lanceolata intermedia, 4.8%), 밀어(Rhinogobius brunneus, 4.4%), 가는돌고기(Pseudopungtungia tenuicorpa, 4.3%) 등의 순으로 우세하였다. 출현종 중 한국고유종은 19종(고유화율 61.3%)이었고, 기후변화민감종(냉수성 어종)은 금강모치(Rhynchocypris kumgangensis)와 둑중개(Cottus koreanus) 2종이 확인되었다. 법정보호종은 환경부지정 멸종위기 야생생물 II급인 묵납자루(A. signifer)와 가는돌고기, 한강납줄 개(Rhodeus pseudosericeus), 둑중개 4종이 출현하였으며, 주로 묵납자루와 가는돌고기는 중·하류에, 한강납줄개는 중류에, 둑중개는 최상류에 서식하고 있었다. 이중 가는돌고기는 출현개체수가 가장 많았고, 전장빈도분포도로 연령을 추정한 결과, 7월기준 전장 24~35㎜는 당년생, 54~75㎜는 1년생, 82~97㎜는 2년생, 104~109㎜는 3년생 이상으로 추정되었다. 군집분석 중 우점도는 상류에서 높고 하류로 갈수록 낮아지는 경향을 보이나 다양도와 균등도, 풍부도는 상류에서 낮고 하류로 갈수록 높아지는 경향을 보였다. 하천건강성(FAI)은 매우 좋음 5개 지점, 좋음 2개 지점으로 나타나 양호하였다. 벽계천은 비교적 서식지가 잘 보존되고 있었으나 멸종위기종이 많이 서식하는 중·하류부 일부는 하천공사가 진행되면서 서식지가 교란되고 있어 보존대책이 요구되었다.

This study was conducted to provide the basic information on environment effects on appearance of ichthyoplankton in the Yeongsan River Estuary in Spring and Summer from 2018 to 2020. Data were obtain from the database of ‘Coastal Ecosystem’ in “National Survey of Marine Ecosystem.” Among the abundance ichthyoplankton species, the Gobiidae spp. dominated, accounting for 85% of the total abundances with the secondary dominant species being the Parablennius yatabei, representing 3% of the abundances. Cluster analysis results revealed a composition differentiated between spring and summer. The Yeongsan River Estuary is known to be significantly influenced by the opening and closing of estuarine gates. In this study, the fluctuation in the number of occurrence groups and abundance among the years and season is attributed to the phenomenon of high water temperature period and the freshwater discharge.

북한 산림황폐화의 원인 중 하나는 1990년대 에너지 위기 이후로 목재에너지를 지나치게 많이 사용한 것으로 지목되고 있다. 그럼에도 불구하고 여전히 땔감문제 해결이 요구되며, 경작지 부족으로 인하여 강하천과 저수지 등 주변 습기가 높은 비경작지에서도 생산성과 발열량이 높은 수종을 식재하여 땔감문제를 해결하려는 노력들이 시도되고 있다. 본 총설에서는 북한의 연료림 조성에 적용 가능한 수종을 대상으로 남북한 기술 현황 검토를 통해 향후 남북산림협력 및 기술 교류 등을 위한 기초자료를 제공하는 것을 목적으로 한다. 북한에서 땔나무림으로 조성되었거나 활용되고 있는 수종으로는 아까시나무, 포플러, 버드나무 및 오리나무 등이 있다. 아까시나무(북한명: 아카시아나무)는 연료림 조성을 위한 주요 수종으로 조림 및 품종 육성 등이 지속적으로 연구되고 있는 것으로 조사되었다. 최근 언론보도 등 문헌을 살펴보면 산림 내 연료림(땔나무림) 조성만으로는 부족한 실정으로 강하천과 저수지 등 비경작지에 식재 가능한 포플러와 버드나무 등 속성수의 육성 품종(상원뽀뿌라나무, 참대버드나무 등)을 활용하고 있는 것으로 나타났다. 남한에서도 포플러류와 버드나무류는 탄소흡수원 확충, 바이오매스 생산 등을 위한 형질개량 및 품종 육성 연구가 수행된 바 있다. 포플러류 중 분포범위가 넓은 사시나무는 근맹아에 의한 번식방법을 이용하여 북한내 많은 지역에서 연료림으로 활용가능할 것이다. 또한 버드나무류의 바이오매스 증진을 위해 종간교잡을 시도한 사례 등 유전개량 및 품종육성을 통한 연료림 조성 기술협력이 가능할 것이다. 속성수를 주거지 인근 비경작지 식재를 위하여 북한에서 원림조성에 활용되고 있는 포플러류와 버드나무류를 활용할 수 있으며, 수확 갱신을 통한 이용방안은 추가적으로 모색되어야 할 것이다.

배경/목적: ROS는 악성종양의 성장 및 공격과 관련이 있다. UDCA는 담도암 세포에서 진행과 전이에 밀접한 EGFR-MAPK 신 호 경 로 와 EMT를 억 제 한 다 . 이 연 구 는 UDCA가 담도암세포에서 ROS 생성 및 그에 관련된 바이오마커에 어떠한 영향을 주는지 알아보기 위해 시행되었다. 방 법 : 인간 간외 담관암 세포주인 SNU-245세포를 배양하였다. 세포생존율은 MTT assays로, ROS는 세포 ROS assays kit로 측정하였다. Western blotting으로 다양한 표적 단백질의 발현 수준을 측정하였다. 특정 유전자의 억제를 위해 siRNA를 사용하였고, 특정 유전자의 과발현을 위해 shRNA를 사용하였다. 결과: UDCA는 담도암 세포에서 DCA에 의한 peroxide와 ROS가 생성되는 것을 억제하였으며, DCA로 발현이 증강된 STAT3, PRX2 및 SOD2를 억제하였고, IGF-1에 의해 발현이 증강된 NOX2 및 NOX4를 억제하였다. 또한, 담도암 세포에서 SiRNA를 이용한 STAT3 및 PRX2의 억제는 UDCA 처치와 상관없이 EGF에 의해 약화된 E-cadherin 발현을 복원하고 EGF에 의해 증가된 N-cadherin 발현을 억제하였는데, 이는 UDCA의 EMT 억제에 PRX2/STAT3가 상당한 역할을 하는 것을 의미한다. 덧붙여, UDCA는 담도암 세포에서 DCA에 의해 억제된 catalase의 발현을 복원하였다. 한편, ShRNA를 사용한 NOX4의 과발현의 유도는 UDCA의 항종양 효과를 상쇄하였다. 결론: UDCA는 담도암 세포에서 ROS 생성을 억제하고, ROS 제거를 향상시킴으로써, 결국 EMT와 관련된 STAT3 및 PRX2를 억제한다, 따라서, UDCA는 ROS 활성도 및 EMT의 억제를 통하여 담도암 세포의 성장 및 침습을 억제하는 데 기여한다.

Semiconductor-based photocatalytic carbon dioxide ( CO2) reduction is of great scientific importance in the field of alleviating global warming and energy crisis. Surface amine modification and cocatalyst loading on the catalyst surface could improve CO2 adsorption capacity and photogenerated charge separation. Herein, amine-modified brookite–TiO2 ( NH2–B–TiO2) coupled metal species (Cu, Ag, Ni(OH)2) cocatalysts have been successfully synthesized by chemical reduction method. The photocatalytic CO2 reduction results show that the CH4 production rates of NH2– B–TiO2/Cu, NH2– B–TiO2/Ag, and NH2– B–TiO2/Ni(OH)2 are 3.2, 12.5, and 1.7 times that of NH2– B–TiO2 (0.74 μmmol g− 1 h− 1), respectively. Results show the introduction of metal species on the surface of the catalyst enhances the absorption range of sunlight and the photogenerated carrier separation efficiency, resulting in enhancing the performance of photocatalytic CO2 reduction. This work provides a strategy for designing metal species-loaded amine-modified brookite–TiO2 by surface/interface regulation to improve photocatalytic efficiency.

The subgenus Tricoma Cobb, 1894 comprises free-living marine nematodes encompassing a total of 83 validated taxa. Within this diversity, twenty-one taxa thrive in the deep sea, while twenty-three are found in coral reefs, flat areas, or green algae. Additionally, eleven taxa inhabit the sublittoral zone at depths exceeding 10 meters, and the remaining taxa are situated on beaches, coasts, or in habitats lacking detailed information. In the course of a survey focused on the East Sea free-living marine nematodes, we identified four new and two previously unrecorded species belonging to the subgenus Tricoma. Specifically, two new species, Tricoma (Tricoma) breviseta sp. nov. and T. (T.) donghaensis sp. nov., were discovered in mud-sandy sediment in deepsea environments below 2000 meters within the Ulleung Basin and Hupo Bank. Two previously unrecorded species [T. (T.) paralucida Decraemer, 1987 and T. (T.) similis Cobb, 1912] and the two newly found species [T. (T.) longicauda sp. nov. and T. (T.) ulleungensis sp. nov.] were obtained from subtidal coarse sand at a depth of 20 meters during a survey of the waters surrounding Ulleungdo Island. The distribution and information on validated taxa within the subgenus Tricoma were systematically collected, reviewed, and cataloged. Detailed morphological features and illustrations of Tricoma species from Korea were provided through the use of differential interference contrast microscopy.

Henricia specimens were collected using a dual approach of trimix scuba diving and fishing nets. This inclusive collection encompasses the discovery of two species highlighted in this study and introduces and provides comprehensive descriptions for Henricia kinkasana and Henricia longispina aleutica. The descriptions offered in this study were derived from the thorough examinations of external morphological characteristics. The documentation provides detailed insight into key traits related to the abactinal and actinal skeletons and spines of these newly recorded species in Korea. This comprehensive examination contributes to our understanding of the distinct morphological characteristics defining each species within the genus Henricia.

Fungal contaminant in the indoor air is one of risk factors that could damage valuable paper-based records preserved in libraries. In the process of monitoring airborne fungi at the Nara Repository, the National Archives, Seoul, Korea, three fungal strains, DUCC 16098, DUCC 17764, and DUCC17767 were isolated from the archive’s air samples. Fungal identification was performed based on the morphological characteristics and phylogenetic analysis of the internal transcribed spacer (ITS), the 28S LSU rDNA, and β-tubulin gene (BenA), and TEF1-α gene. These DUCC 16098, DUCC 17764 and DUCC17767 strains were identified as Clonostachys farinosa, Penicillium cosmopolitanum, and Cephalotrichum purpureofuscum. These species have not been recorded before in Korea. Information on these fungi will help the monitoring and management of airborne fungi in the archival facilities.

Air conditioner filters purify the air of indoor environments by removing air pollutants and supporting the efficiency of the unit’s cooling function. However, an air conditioner filter can become a microenvironment in which some fungi can grow as dust continues to accumulate and favorable humidity conditions are formed. Fungal growth in air conditioner filters could lead to fungal allergies or fungal diseases, in addition to emitting a foul odor. In an effort to understand what species causes this malodorous problem, we investigated the diversity of fungi found in air conditioners. Fungi were sampled from the collected air conditioner filters and grown on DG18 agar media. After purification for pure isolates, species identification was undertaken. Colony morphology was observed on PDA, MEA, CYA, and OA media. Microstructures of fruiting body, mycelia, and spores were examined using a light microscope. Molecular identification was performed by PCR and sequencing of PCR amplicons, and molecular phylogenetic analysis of sequenced DNA markers, including the Internal Transcribed Spacer (ITS), the 28S large subunit of the nuclear ribosomal RNA (LSU rDNA), the β-tubulin (BenA) gene, the Calmodulin (CaM) gene, and the DNA-directed RNA polymerase II subunit 2 (RPB2) gene. Through this identification process, we found two fungal species, Aspergillus miraensis and Dichotomopilus ramosissimus, which are unrecorded species in Korea. We will now report their morphological and molecular features.

Background: Largemouth bass (Micropterus salmoides) is introduced species that has caused aquatic ecology activity both in vitro and in vivo were investigated for the possibility of application of the bass extract as an alternative feed ingredient. Methods: The bass oil was extracted using a 1-L supercritical extractor, while the protein was extracted from 250 g of bass dry matter, which was dissolved in 1 mL of H2O at 50℃. Both oil and protein extracts were evaluated antioxidant activities and the level of DPPH radical scavenging assay and nitric oxide (NO) production assay with lipopolysaccharide response. Oral administration of 6.6 μL/g bass protein and 5.38 μL/g bass oil conducted for investigating serological and physiological effect. Results: DPPH radical scavenging showed similar radical scavenging ability of 50 μM of ascorbic acid at 200 μg of protein and 10% of oil treatment. NO concentration was diminished by the treatment of bass oil. Oral administration of both bass oil and proteins to mice showed that the body weight increase rate of the bass oil treated group was significantly reduced by 1.55 g compared to the other groups. The number of white blood cells (WBC) was increased by 4.52 k/μL in the bass protein-treated group and 4.44 k/μL in the bass oil-treated group compared to the control group. However, the serum IgG level did not show a significant difference between the bass extract-treated groups and the control group. Conclusions: These studies demonstrate that both bass oil and proteins extracted from the bass not only provide excellent effects of antioxidant and immune activity but can also be used as functional food supplements.

해양공간계획(Marine spatial planning)은 해양의 합리적인 이용과 지속 가능한 해양 공간 활용을 위한 중요한 요소이다. 특히 어업활동 보호구역은 지속 가능한 어업을 위한 핵심 용도구역으로, 해양공간계획 경계 내에서 약 45.6%를 차지한다. 그러나, 현재 어업활동보호구역의 지정 과 평가는 미래 수요와 잠재적 가치를 충분히 반영하지 못하고 있으며, 중장기 계획 수립을 위한 보다 합리적인 평가 방법과 예측 도구가 필요한 상황이다. 이러한 문제를 해결하기 위해, 본 연구는 어업활동보호구역 내 주요 어종인 고등어, 갈치, 멸치, 참조기를 대상으로 어종 분포 예측을 시도하고, 현재 용도구역과의 비교를 통해 예측 도구의 가능성을 평가하였다. 한편, IPCC 6차 기후변화 시나리오(SSP1-2.6 및 SSP5-8.5)를 적용한 종분포 모델(MaxEnt)을 사용하여 미래 기후변화에 따른 어종의 이동 및 분포 변화를 분석한 결과, 고등어, 갈치, 참조기의 분포 면적은 현재보다 약 28~86% 증가했으나, 멸치의 분포 면적은 약 6~11% 감소했다. 이 결과를 바탕으로 주요 4종의 종풍부도 지도를 작성하였으며, 해양공간계획 경 계 내에서 ‘높음’으로 평가된 종풍부도 해역과 어업활동보호구역이 중복되는 비율은 약 15%, SSP1-2.6 시나리오에서 21%, SSP5-8.5 시나리오에서 34%로 증가하였다. 연구 결과는 향후 용도구역 평가나 유보구역 변경 시 과학적 근거로 활용될 수 있으며, 어종의 현재 종분포와 기후변화에 따 른 분포 예측을 통해 현재 용도구역 평가의 한계를 보완하고, 지속 가능한 유용 해양 자원의 이용을 위한 계획 수립에 기여할 것으로 판단된다.

River estuaries are dynamic and productive ecosystems with high regional biodiversity. Environmental DNA (eDNA) has become a useful approach to assessing biodiversity in aquatic ecosystems. This study was conducted to investigate fish community characteristics and species diversity in two river estuary ecosystems, the Taehwa River and Changwon Stream. We further compared conventional and eDNA metabarcoding analyses of the fish communities. The conventional survey was performed in May, July, and October 2022, while the eDNA analysis was conducted only in May. We observed various fish species with different life histories, including carp, goby, and marine fish. We also found that migratory fish, such as dace Tribolodon hakonensis, sweetfish Plecoglossus altivelis, and eel Auguilla japonica, occurred in the Taehwa River, suggesting high river connectivity. Marine fish species were predominant in the Changwon Stream, as this river is located close to the sea. The diversity indices showed that the Taehwa River generally had higher species richness, evenness, and diversity values than the Changwon Stream. A total of 9-19 species were detected in the conventional survey for the three sites, whereas 11-18 species were found from eDNA analysis. The findings indicate that the sensitivity of eDNA was similar to or higher than that of the conventional method. Our study findings suggest the efficiency and efficacy of eDNA-based fish community monitoring, although with some shortcomings in applying the genetic marker to Korean fish, including no clear-cut distinction for Korean endemic species and/or genetically closely related species groups.

Species distribution model (SDM) is used to preserve biodiversity and climate change impact. To evaluate biodiversity, various studies are being conducted to utilize and apply SDM. However, there is insufficient research to provide useful information by identifying the current status and recent trends of SDM research and discussing implications for future research. This study analyzed the trends and flow of academic papers, in the use of SDM, published in academic journals in South Korea and provides basic information that can be used for related research in the future. The current state and trends of SDM research were presented using philological methods and text-mining. The papers on SDM have been published 148 times between 1998 and 2023 with 115 (77.7%) papers published since 2015. MaxEnt model was the most widely used, and plant was the main target species. Most of the publications were related to species distribution and evaluation, and climate change. In text mining, the term ‘Climate change’ emerged as the most frequent keyword and most studies seem to consider biodiversity changes caused by climate change as a topic. In the future, the use of SDM requires several considerations such as selecting the models that are most suitable for various conditions, ensemble models, development of quantitative input variables, and improving the collection system of field survey data. Promoting these methods could help SDM serve as valuable scientific tools for addressing national policy issues like biodiversity conservation and climate change.

Stomatal pore is an important physiological trait that is closely linked to photosynthesis and transpiration as carbon dioxide and water vapor move through it between the atmosphere and plants. The present study investigated stomatal traits, such as stomatal density, index and size, of herbaceous native and alien plant species living in a riparian park on the Nakdong River to understand how those traits vary and to know if successful settlement of alien plants is attributed to those traits. There was no difference in stomatal density, index and size between native and alien plants with kidney-shaped stomata, suggesting that an empty ecological niche is not an essential prerequisite for the successful settlement of alien plants. Stomatal density showed a negative correlation with leaf thickness and leaf dry weight content (LMDC), but there was no correlation with Specific leaf area (SLA). All plants with kidney-shaped stomata had amphistomatous leaves, and the density and size of dumbell-shaped stomata were lower than those of kidney-shaped stomata.

The limitation in deriving the species richness representing the entire country of South Korea lies in its relatively short history of species field observations and the scattered observation data, which has been collected by various organizations in different fields. In this study, a comprehensive compilation of the observation data for plants held by agencies under the Ministry of Environment was conducted, enabling the construction of a time series dataset spanning over 100 years. The data integration was carried out using minimal criteria such as species name, observed location, and time (year) followed by data verification and correction processes. Based on the integrated plant species data, the comprehensive collection of plant species in South Korea has occurred predominantly since 2000, and the number of plant species explored through these surveys appears to be converging recently. The collection of species survey data necessary for deriving national-level biodiversity information has recently begun to meet the necessary conditions. Applying the Chao 2 method, the species richness of indigenous plants estimated at 3,182.6 for the 70-year period since 1951. A minimum cumulative period of 7 years is required for this estimation. This plant species richness from this study can be a baseline to study future changes in species richness in South Korea. Moreover, the integrated data with the estimation method for species richness used in this study appears to be applicable to derive regional biodiversity indices such as for local government units as well.