겨울철 저온에 의한 식물의 반응 및 생리적 상태를 확인하기 위해 문주란(Crinum asiaticum var. japonicum)을 대상으로 엽록소형광과 항산화효소 찬성의 변화를 조사하였다. O-J-I-P곡선을 분석한 결과 겨울철에 J, I, P-단계에서의 형광세기가 현저하게 간소하였다. 문주란 잎의 광계II의 광화학적 효율, 즉 Fv/Fm은 겨울철의 온도변화 추이와 유사하게 낮은 값을 나타내어 겨울철 저온이 스트레스 요인으로. 작용하는 것으로 보인다.

In order to establish the processing condition of salt-fermented liquefaction of sardine (Sardinops melanoslicta), effect of temperature, pH value, and concentration of salinity on crude enzyme activity of sardine viscera were investigated. The optimum temperature range of crude enzyme activity in sardine viscera was 45~50℃ and the optimum pH value of it was 9.8. According to the concentration of salinity increased the crude enzyme activity in sardine viscera decreased. The relationship between concentration of salinity (X) and the crude enzyme activity (Y) in sardine viscera is shown as follows; Y=-0.01363X+0.7676 (r=-0.88). For the purpose of processing conditions of rapid- and low salt-fermented liquefaction of sardine, changes of viable cell count, histamine content, and volatile basic nitrogen (VBN) in the chopped whole sardine with 8% NaCl during preheating process at 40˚, 45˚ and 50℃ for 48 hrs were analyzed. During preheating, initial viable cell counts of chopped whole sardine were 104-7/g, but they decreased 101-5/g after 48 hrs. Histamine contents during preheating process at 40˚ and 45℃ were gradually increased, whereas at 50℃ were almost the same level after 48 hrs. VBN contents were continuously increased during preheating, but preheating at 50℃ samples were lower level than that of 40˚ and 45℃ ones. For the purpose to accelerate the fermentation and liquefaction of chopped whole sardine, preheating at optimum temperature of crude enzyme activity for 48 hrs was useful processing method and the contents of viable cell count, histamine, and VBN were safety level for food sanitation.

To investigate the effect of feeding rats medium-chain triglyceride(MCT), triglyceride containing primarily C8 and C10 fatty acids, it were compared to the effects of feeding triglycerides composed of long-chain triglyceride (LCT), corn oil and lard, on the serum enzyme activity. For 4 weeks rats fed a diet containing 20％ MCT or LCT · MCT, as compared with LCT, had the following effects: 1) The total lactic dehydrogenase(LDH) activities in the serum of all experimental group were significantly decreased then those of control group. 2) The activities of glutamic oxaloacetic transaminase (GOT) in the serum of all experimental group were decreased than those of control group. 3) The activities of glutamic pyruvic transaminase (GPT) in the serum of all experimental group were decreased, MCT and LCT group were singinficantly decreased than of control group. 4) The activities of α-amylase in the serum of all groups were significantly increased than those of control group. 5) According to electrophoresis, LDH of LDH isoezyme activities in MCT and Lard group were increased with those of LDH5 in corn oil group were increased than those of control gourp. It is suggested that the MCT and LCT fed to rats influence on the activity of various serum enzymes.

Tomato과실(果實)의 성숙단계별(成熟段階別), pectin효소(酵素)(PE, PG)의 활성(活性)의 변화(變化)와 polygalacturonase (PG)의 분리(分離), 정제(精製) 및 정제(精製)된 PG isoenzyme의 효소학적(酵素學的) 성질(性質)에 대하여 조사(調査)하였다. Tomato과실(果實)의 pectin esterase (PE)효소(酵素)는 추숙(追熟)이 진행(進行)됨에 따라 그 활성(活性)이 증가(增加)하였으며, 특히 색깔의 변화가 일어나는 시기(時期)에 최대(最大)의 활성(活性)을 나타내었다. PG는 녹숙과(綠熟果)에서 활성(活性)이 거의 나타나지 않았으나 추숙(追熟)이 진행(進行)되면서 크게 증가(增加)하였다. Ripe tomato에서 추출(抽出)한 조효소액(粗酵素液)을 전기영동(電氣泳動)시킨 결과(結果) 6개의 단백질(蛋白質) band를 나타내었다. 조효소액(粗酵素液)을 황산암모니아에 의한 단백질(蛋白質)의 분획(分劃) DEAE-sephadex A-50 column chromatography한 결과(結果) 2개의 isoenzyme I와 II (PG I, PG II)를 분리(分離)하였으며, isoenzyme I와 II는 각각 61배, 98배 정제(精製)되었다. Isoenzyme I와 II는 전기영동상(電氣泳動上) Rm값은 각각 0.25와 0.31이었다. Isoenzyme I의 최적온도(最適溫度) 및 pH는 각각 , pH 4.5에서, isoenzyme II는 각각 , pH 4.7에서 최대활성(最大活性)을 나타내었다. Isozyme I에 대한 pH와 열안정성(熱安定性)을 조사(調査)한 결과(結果), pH 4.3부근에서 안정(安定)하였으며, , 5분간(分間)에서 약 50%의 효소활성(酵素活性)이 억제되었다. 그리고 isozyme II에 대한 pH와 열안정성(熱安定性)을 조사(調査)한 결과(結果), pH 5.6 부근에서 안정(安定)하였으며 , 5분간(分間)에서 약 50%의 효소활성(酵素活性)이 억제(抑制)되었다. Isozyme I와 II에 대한 최적(最適) NaCl농도(濃度)는 각각 0.15M, 0.1M이었으며, 최적(最適) 농도(濃度)는 각각 0.04M, 0.03M이었다.

In order to investigate the acute toxicity of autoxidized methyl linoleate(AOML) on the activity of serum enzymes in the mouse, we administered once 0.45ml of AOML to ICR strain mouse by using stomach tube. The following results were obtained: The total lactic dehydrogenase(LDH) activities in the serum of AOML group were generally increased than those of normal group. According to electrophoresis, the activities of LDH, were increased while those of LDH, were decreased. The activities of glutamic oxaloacetic transaminase(GOT), glutamic-pyruvic transaminase(GPT) and α-amylase in the serum of AOML group were increased more than those of normal group. The activities of alkaline phosphatase in the serum of AOML group were increased but those of isozyme were not confirmed in the normal and AOML group. In the serum protein of AOML group, albumin was increased, on the other hand γ-globulin was decreased. At the peripheral blood slide smear, lymphocytes were significantly decreased but neutrophils were increased and the morphological change of erythrocytes was observed. From these results we conclude that the AOML fed to mouse influences on the activity of various serum enzymes and blood cells in the mouse.

Background: To enhance the taste and physiological characteristics of Lycii fructus (Gugija) extracts, we investigated the changes in the physiological characteristics of Gugija extracts caused by adding white ginseng (WG) and red ginseng (RG) Methods and Results: Gugija extracts, including 10G10, 10GW-G8 : 2, -G6 : 4, -G4 : 6, -G2 : 8, and -G0 (mixtures made by replacing 0, 20, 40, 60, 80, and 100% of Gugija with WG), as well as 10G10, 10GR-G8 : 2, -G6 : 4, -G4 : 6, -G2 : 8, and -G0 (mixture made by replacing 0, 20, 40, 60, 80, and 100% of Gugija with RG) were extracted with water at 10 times the respective mixture's volume. The antioxidant activities of Gugija extracts were investigated by assessing their 1,1-diphenyl-2-picrydrazyl (DPPH) and 2,2’-azinobis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant potential (FRAP) activity, nitrite scavenging activity, and angiotensin converting enzyme (ACE) inhibitory activity. As the amount of WG added increased, the DPPH, and, ABTS radical scavenging activity, and FRAP activity of the Gugija extract decreased. The half maximal inhibitory concentration (IC50) value of 10G10, 10GW-G6 : 4, 10GR-G6 : 4, and 10GR-G0 for DPPH radical scavenging activity were 25.50 ± 1.04, 52.06 ± 1.46, 16.87 ± 1.24, and 9.50 ± 0.16 ㎕/㎖, respectively. On the other hand, the physiological activity of Gugija extract increased with the addition of increasing amounts of RG. However, ACE inhibitory activity was the highest (50.25 ± 2.58%) in the Gugija 10-fold extract without any added RG. Conclusions: From the above results, we suggest that adding RG to Gugija extracts increase their antioxidant, FRAP, and nitrite scavenging activities.

This study aimed to produce fermented soy-powder milk (FSPM) with Lactobacillus plantarum P1201 and to evaluate its anti-obesity activity. Isoflavone and conjugated linoleic acid (CLA) of unfermented soy-powder milk (UFSPM) and FSPM and were analyzed via high-pressure liquid chromatography (HPLC) and gas chromatography (GC). Their inhibitory activities against α-glucosidase, α-amylase, and pancreatic lipase were assayed. Their anti-obesity activities were evaluated on the basis of their inhibitory effects on adipocyte differentiation in 3T3-L1 cells, and the expression of mRNAs associated with adipogenesis and lipid metabolism were analyzed via real time-polymerase chain reaction (RT-PCR) and quantitative PCR (qPCR). FSPM with L. plantarum P1201 increased the isoflavone aglycones (daidzein, glycitein, and genistein) content and produced CLA in soy-powder milk (SPM), both of which possessed bio-activity. Both UFSPM and FSPM showed dose-dependent inhibitory activity for α-glucosidase, α-amylase, and pancreatic lipase. FSPM, but not UFSPM, suppressed adipogenesis in 3T3-L1 cells and reduced their triglyceride content by 23.1% after treatment with 1,000 μg/mL of FSPM, compared with the control group. The anti-obesity effect of FSPM can be attributed to CLA and isoflavone aglycones, which targeted CCAAT/enhancer binding protein α (C/EBP-α) and down-regulated lipoprotein lipase (LPL), adiponectin, adipocyte fatty acid-binding protein (aP2), fatty acid synthase (FAS), and acetyl CoA carboxylase (ACC) mRNA. Furthermore, FSPM enhanced the inhibitory activity of glucosidase and pancreatic enzymes and anti-obesity activity. Further studies are required to investigate whether the anti-obesity effect of FSPM persists in an in vivo mouse model of diet-induced obesity.

This study investigated the nutritional properties and biological activities of Ganoderma lucidum (GL). The round type of GL contained higher carbohydrate content, while the Nokgak type of GL contained higher crude ash, crude fat, and crude protein content. The most abundant amino acid, fatty acid, mineral, and soluble vitamin observed were valine (round type: 11.90 mg/g and Nokgak type: 17.18 mg/g), linoleic acid (round type: 47.56% and Nokgak type: 75.68%), potassium (round type: 116.50 mg/100 g and Nokgak type: 184.36 mg/100 g), and vitamin B3 (round type: 1.78 mg/100 g and Nokgak type: 1.81 mg/100 g), respectively. In addition, the β-glucan content were 34.15 g/100 g (round type) and 30.07 g/100 g (Nokgak type). The GL 70% ethanol extract at 40℃ showed higher radical scavenging as well as carbohydrate and lipid enzyme inhibition than other conditions. At 1 mg/mL of treatment with the 70% ethanol extract at 40℃ of round type GL, the DPPH, ABTS, hydroxyl radical scavenging, and α-glucosidase, α-amylase, and pancreatic lipase inhibition activities obtained were approximately 92.85, 99.74, 58.09, 89.68, 44.68, and 67.56%, respectively.

The purpose of current study is to investigate the beneficial effect of enzyme (Alcalase) hydrolysates of silk protein in rat. Alcalase-treated silk protein hydrolysate (ATSH) itself did not show any cytotoxicity on the hepatic tissues and blood biochemistry, similar to the normal condition. ATSH played a protective role in tert-butyl hydroperoxide (t-BHP)-induced hepatotoxicity and liver damage. The values of AST (aspartate aminotransferase) and ALT (alanine aminotransferase), which are the indicators of the liver function, were effectively alleviated with the ATSH treatment in a dose dependent manner. The level of Lactate dehydrogenase (LDH) and Malondialdehyde (MDA), which were increased with t-BHP treatment, were significantly reduced by ATSH. High dose of ATSH (2 g/kg) reduced the t-BHP-induced LDH release by 48%. Antioxidant and antioxidant enzymes in liver cells were significantly increased by ATSH treatment in their level and activities. ATSH (2 g/kg) increased glutathione (GSH), an intracelluar antioxidant, by 2.5-fold compared with the t-BHP treated group. The activities of glutathione-s-transferase (GST), superoxide dismutase (SOD), and catalase were also elevated by 38%, 60%, and 45%, respectively, with ATSH (2 g/kg) treatment. The antioxidative effect of ATSH was recapitulated to the protection from t-BHP induced liver damages in hematoxylin and eosin (H&E) staining. Thus, ATSH might be used as a hepatoprotective agent.

두둑과 고랑을 재활용한 한국형 무경운 농업에서 유기물 투입과 관수 효과를 구명하고 자 무경운 토양에서 시험을 수행하였다. 1. 토양 미생물상 1회 전량관수 조건에서 대두박 투입 처리구의 토양 세균과 곰팡이 수는 대두박 무 투입 구에 비하여 많았다. 그리고 유기질비료 투입량이 표준시비량 66%까지 증가되면 세균과 곰팡이 수는 증가되었으나, 그 이상에서는 세균과 곰팡이 수가 감소되는 경향이었다. 곰팡 이/세균 비율은 관수 방법과 관계없이 대두박 투입 처리에서 0.6과 1.1로, 무투입 처리의 0.2와 0.5보다 2배 이상 높았다 1회 전량 관수 조건에서 유기질 비료 시비량이 증가되면 대두박을 투입한 처리는 방선균 수는 감소되는 경향이었으나, 대두박 무투입에서는 증가되었다. 2회 분할 관수는 1회 전량 관수에 비하여 대두박 무 투입 조건에서 세균과 곰팡이 수가 증가되었으나, 대두박 투입 조건에서는 방선균 수가 증가되었다. 2. 토양 효소 유기질 비료의 시비량이 증가되면 토양 내 Chitinase 활성은 대두박 투입 토양에서 감소 되고, 대두박 무 투입에서는 증가되는 경향이었다. 그러나 대두박을 투입에 관계없이 2회 분할 관수는 1회 전량관수에 비하여 Chitinase 활성이 증가되었다. 1회 전량관수 조건에서 대두박 투입 처리구의 β-Glucosidase 활성은 무투입에 비하여 높 았으며, 유기질 비료 투입량이 증가되면 표준시비량의 66%까지는 β-Glucosidase 활성이 증 가되었으나, 표준시비량에서는 감소되었다. 대두박 무투입 조건에서 2회 분할관수 토양 내 β-Glucosidase 활성은 1회 전량관수에 비하여 현저하게 증가되었다. 1회 전량관수 조건에서 대두박을 투입한 처리의 N-acetyl-β-D-glucosaminidase의 활성은 무투입구에 비하여 높았다. 대두박 투입 처리에서 유기질 비료 투입량이 표준시비량의 66 %까지 증가되면 N-acetyl-β-D-glucosaminidase의 활성은 증가되었으나, 표준시비량에서는 유의적인 차이가 없었다. 대두박 무투입 조건에서 2회 분할관수는 1회 전량관수에 비하여 N-acetyl-β-D-glucosaminidase의 활성은 증가되었다. 대두박 무투입 조건에서 유기질 비료 시비량이 표준량의 66% 수준에서는 토양 내 산성인 산가수분해효소(Acid phosphatase)의 활성 높았다. 대두박 투입 조건에서는 유기질 비료 시비 량이 증가되면 산성인산가수분해효소(Acid phosphatase)의 활성은 증가되는 경향이었다. 3. 토양 AMF 대두박 무투입 조건에서 유기질 비료의 투입량이 표준시비량의 66%까지 증가되면 토양 의 내생균근균의(AMF) 포자수는 증가되었으나, 유기질 비료 투입량이 표준시비량에서는 근균의 포자수는 감소되었다. 그러나 대두박 투입에서 근균의 포자수는 유기질 비료 투입 량에 따른 유의적인 차이가 없었다. 그리고 내생 근균의 고추 뿌리에 정착률은 대두박 투 입량에 따른 유의적인 차이가 없었으며, 2회 분할 관수도 같은 경향이었다.

The microbial composition in Nuruk, a Korean cereal fermentation starter, is a critical factor for the quality and organoleptic properties of traditional alcoholic beverages. This study was aimed at monitoring the compositional change and enzyme activity of culturable lactic acid bacteria (LAB) in two types of Nuruk fermented at different temperatures. All culturable LAB were isolated at various time points (0, 3, 6, 10, 20, and 30 days) and identified by 16S rRNA sequencing. In traditional Nuruk type A (TN-A), which was fermented at 36℃, the population of total culturable LAB during the fermentation period was between 104 and 105 log CFU/mL. On the other hand, the LAB population in traditional Nuruk type B (TN-B) fermented at 45℃ (primary fermentation for 10 days) and 35℃ (secondary fermentation for 20 days) was 102 log CFU/mL; however, these bacteria could not be detected after 6 days. Major LAB strains were identified in both Nuruk types: (1) from the MRS-culture of TN-A, Pediococcus pentosaceus at 3-30 days; (2) from MRS-culture of TN-B, P. pentosaceus at 3 days and Enterococcus hirae at 6 days. The protease activities of the dominant LAB isolated from the TN-A and TN-B cultures were within the ranges of 0.64~1.03 mg/mL and 0.74~0.81 mg/mL (tyrosine content), respectively, whereas the α-amylase activities were 0.75~0.98 mg/mL and 0.78~0.79 mg/mL (amylose content), respectively.

This experiment was conducted to obtain the have higher contents of pharmaceutical constituents as well as higher yield from colchicine induced diploid and tetraploid extracts of Platycodon grandiflorum. In order to determine the biological activity, this study was focused to evaluate the cytotoxicity, antimicrobial on the bronthus disease bacteria, antioxidant enzyme activity of diploid and tetraploid extracts in P. grandiflorum. The activities of antioxidant enzyme according to different solvent extracts were measured as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The cytotoxicity of methanol extracts of P. grandiflorum showed significant differences between tetraploid and diploid. That is, the cytotoxic effect against human cancer cell was higher in tetraploid than in diploid. At all extracts concentration, tetraploid samples showed high toxicity and the IC50 (concentration causing 50% cell death) value showed the highest on HCT-116 cell (105.91 μg/mL), and exhibited significant activity against the Hep 3B cell (140.67 μg/mL), SNU-1066 cell (154.01 μg/mL), Hela cell (158.37 μg/mL), SNU-601 cell (182.67 μg/mL), Calu-6 cell (190.42 μg/mL), MCF-7 cell (510.19 μg/mL). Antimicrobial activities of diploid P. grandiflorum were relatively low compared to tetraploid P. grandiflorum on most of the bacterial strains. In tetraploid P. grandiflorum, K. pneumoniae showed the clear zone formation (18~19 mm) of growth inhibition, followed by the clear zone formation of 13~15 mm on C. diphtheria and S. pyogenes. The antimicrobial activities in diploid P. grandiflorum were the highest on K. pneumonia (14~15 mm), and showed the clear zone formation of 11~12 mm on C. diphtheria and 12~13 mm on S. pyogenes. The antimicrobial activity is thought to look different depending on the bacterial strains and the polyploidy of P. grandiflorum. The root extract of P. grandiflorum had the highest (97.2%) SOD enzyme activity in ethyl acetate partition layer of tetraploid while water partition layer of diploid showed the lowest (48.6%) SOD enzyme activity. The activity of CAT showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all partition layers except butyl alcohol. The activities of APX and POD showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all fraction solvents except water layer. These results indicate that the tetraploid P. grandiflorum can be used as a source for developing cytotoxic agent and antimicrobials which can act against bronchus diseases bacterial strains.