PURPOSES: The scaling of a concrete surface caused by the combined effects of frost and de-icing salts is one of the main reasons for the need to repair transportation infrastructures in cold-climate regions. This study describes the results of attempts to determine the scaling resistance of concrete incorporating mineral admixtures such as fly ash, GGBFS, and silica fume, and subjected to the actions of frost and salt. METHODS : Conventionally, to evaluate the fundamental properties of concrete, flexural and compressive strength measurements are regularly performed. Based on the ASTM C 672 standard, concrete is subjected to 2%, 4%, and 8% CaCl2 salt solutions along with repeated sets of 50 freeze/thaw cycles,and the scaling resistance was evaluated based on the mass of the scale and a visual examination. RESULTS : It was observed that silica fume is very effective in enhancing the scaling resistance of concrete. Meanwhile, concrete incorporating GGBFS exhibited poor resistance to scaling, especially in the first ten freeze/thaw cycles. However, fly ash concrete generally exhibited the maximum amount of damage as a result of the frost-salt attack, regardless of the concentrations of the solutions. CONCLUSIONS: It can be concluded that the scaling resistance of concrete is highly dependent on the type of the mineral admixture used in the concrete. Therefore, to provide a durable concrete pavement for use in cold-climate regions, the selection of a suitable binder is essential.

Eight minerals (Ca, Cu, Fe, K, Mg, Mn, Na and Zn) were determined in Schizandra chinensis fruits and their infusions in Seoul. The average mineral contents per Schizandra chinensis fruits (100 g) were K (966.08 mg), Mg (87.20 mg), Ca (15.19 mg), Mn (6.19 mg), Fe (3.99 mg), Zn (2.78 mg), Na (2.15 mg) and Cu (0.31 mg) respectively by Inductively Coupled Plasma-Mass Spectrometer. The quantity of K and Zn were found significantly higher in Korean fruits than the Chinese fruits (p < 0.05). The other 6 minerals did not reveal remarkable difference. In the case of processed Schizandra chinensis tea, it had K (217 mg), Mg (20.40 mg), Ca (7.16 mg), Na (2.95 mg), Mn (1.34 mg), Fe (0.95 mg), Zn (0.25 mg) and Cu (0.09 mg) per 100 g respectively. In extraction of Schizandra chinensis fruits, as it took a longer extraction time, and in a powder form rather than a raw form, it had a higher contents of minerals. When a powder type of Schizandra chinensis fruits was extracted for 24 hours at 25℃, the transfer rate appeared with Ca (36.27%), Mn (25.44%), K (22.43%), Zn (21.00%), Na (19.91%), Mg (19.55%) Cu (13.78%) and Fe (6.45%) respectively.

The objective of this research was to investigate the effect of Taekwondo training for 16 weeks on obese elementary school students’ growth hormone and bone mineral density(BMD). The study was conducted on male obese elementary school students who reside in D metropolitan city and have body mass index (BMI) higher than 25%. The whole subject group had no particular diseases and did not regularly exercise more than twice a week. Based on basal movement, low section, Taekwondo gym and form pattern, the Taekwondo train program was composed of total 16 weeks; 4 weeks of introductory phase, 4 weeks of maintenance phase and 4 weeks of improvement phase one and two, respectively. BMD was analyzed using Dual-energy X-ray absorptiometry standard protocol. As a result, growth hormone was not significantly difference in group and exericse effects. In BMD, there was a significant difference between the two groups in BMD lumber spine(LS). In BMD-femur, there was a significant difference in the group (p<0.01) and time (p<0.05). In body composition, there was no statistically significant difference between body mass, body mass index, body fat mass and muscle volume. In conclusion, since 16 weeks of Taekwondo training had a positive effect on elementary students’ metabolic syndrome index, BMD and postural balance. We can deduce that elementary students need constant physical activity for their growth, physical development and physical health.

As people seek a healthy, wealthy, and quality life more than before, interests in mineral water are picking up and the consumption continues to increase accordingly. In an experiment to examine changes in the water quality after mineral water on the market was kept indoor and outdoor for four weeks, there seemed to be no problem in external appearance. Water kept in an outdoor storage contained more odor-savor than water kept in an indoor storage. Odor was not detected in 90% of the sample, and savor not in 80% of it. As for the mechanism of odor-savor generation, the elution of chemical substances from a container may be taken into consideration. In general, odor and savor have turned out to be in close correlation to each other. The average number of bacteria in the sample mineral water was 200±10 CFU/mL, and such factors as area of storage and ozone post-treatment did not cause significant effects on the reproduction of common microbes. Hence, it is thought that strict regulations on a production process and reduction of the period of circulation will contribute to customers consumption rates.

In order to use as a new functional food material, we analyzed the chemical components including the organic compounds, minerals and Vitamin C of cherry and acacia honey which were produced in South Korea. The condensed rate of methanol extraction in honey was 87.51% of cherry honey and it was 93.06% of acacia honey. In the case of cherry honey, main organic compounds that extract by organic solvents in GCMS analysis were trichloromethane, propylcarbinol, methacide, cyclopentane, tetrafinol etc. and main aromatic compounds that extract by organic solvents in SPME analysis were formyl trichloride, propanal, furfurylaldehyde, pyrazole, benzenecarbonal etc. Also, in occasion of acacia honey, main organic compounds were trichloromethan, Acetoxyethane, Hexanaphthene, acetidin etc. and main aromatic compounds were Hydrazomethan, Azulene, Cyclotrisiloxane, Hydrazine etc. Proximate composition was crude protein 0.33%, crude fat 0.15%, crude ash 0.47% in cherry honey and crude protein 0.10%, crude fat 0.44%, crude ash 0.06% in acacia honey. Free sugar that analyze by HPLC was fructose 37.05%, glucose 27.29%, total sugars 64.34% in cherry honey and fructose 48.52%, glucose 24.29%, total sugars 72.81% in acacia honey. Vitamin C was not detected in two sample honeys. Minerals by ICP analysis were detected total 25 kinds in cherry honey, K 9.762 ppm¤Si 5.628 ppm ¤Na 5.096 ppm¤Ca 2.224 ppm etc. and total 22 kinds in sacacia honey, Na 4.527 ppm¤Si 3.420 ppm¤K 3.091 ppm¤Zn 1.482 ppm etc.

효과적인 광물자원 탐사 기술을 개발하기 위하여 광물자원의 포텐셜 지도 구축 연구를 수행하였다. 대상 지역은 광상 성인모델이 알려진 가곡광산으로 선정하였다. 성인모델을 바탕으로 관계화성암, 단층, 탄산염암 등의 지질학적 요인들을 평가 인덱스로 선정하였다. 각 요인들과 광체간의 공간적 상관관계를 분석하여 각 지질 구조 들로부터의 거리에 따른 가중치를 해석, 가곡광산에 대한 포텐셜 지도를 구축하였다. 해석된 포텐셜 지도는 현재 알려진 광체의 위치에서 높은 포텐셜 값을 갖으며, 높은 포텐셜 영역의 패턴 역시 광체 패턴과 유사하게 나타나고 있다. 이 결과는 추후 수행될 자원 탐사 시 현재 알려진 광체와 유사한 지질환경을 갖는 영역을 효과적으로 제시할 수 있기 때문에 그 효용성이 높다 할 수 있다.

This study was carried out investigate the effects of dietary addition of mineral and sugar on the dry matter intake, daily gain, yield grade and quality grade of Hanwoo (Bos taurus coreanae) steers. Three diets fed to steers included a control diet (concentrate mix and rice straw : C) and two treatments diet (control diet + black sugar 100 g + mineral 100 g : T1, and control diet + black sugar 150 g + mineral 50 g : T2). The results are summarized as follows; total feeding intake, body weight gain and daily gain did not show significant differences among the three treatments. Cold carcass weight was significantly (p<0.05) higher in T2 than in the other two treatments (C and T1). There was no significant difference in yield traits of back fat thickness, longissimus muscle area and yield grade among the three treatments (C, T1 and T2). Marbling score showed significantly (p<0.05) higher in order of T2 (5.67) > T1 (4.67) > C (3.67). Meat color, fat color, texture and maturity were no significant difference. Quality grade was higher in T2 than in the other two treatments (C and T1), but there was no significant difference. The results show that marbling score and quality grade of Hanwoo can be increased by high dry matter intake with feeding addition of mineral and sugar.

The purpose of this study was to investigate the effect of calcium (Ca) and vitamin D (vit. D) levels on metabolism of various minerals such as Ca, P, Mg, Fe, Zn, Cu, and Cr. The comparison was made on the rats that were placed on diet containing powdered skim milk with different Ca and vit. D levels for 5 weeks. A total of 42 5-week-old Sprague- Dawley rats were divided into 7 groups as follows: Control group consisted of normal Ca and normal vit. D (0.5% Ca, 1,000 IU vit. D); Experimental groups were divided into low (0.25%) and high (1.0%) calcium levels; and vit. D group was divided into low (10 IU), normal (1,000 IU), and high (5,000 IU) subgroups. The weight gain and food efficiency ratios of the rats were not significantly different with increasing dietary Ca levels. The absorption rates of 7 minerals (Ca, P, Mg, Fe, Zn, Cu, and Cr) were significantly decreased with increasing dietary Ca levels. Also, fecal excretion of P significantly increased with increasing dietary vit. D levels (p<0.05), and urine excretion of Fe was significantly increased with increasing dietary vit. D levels (p<0.001). The result indicated that higher Ca intake affected on bioavailability of other minerals, due to interactions among minerals in the process of intestinal absorption. However, vitamin D intake had no effect on bioavailability of several minerals. Therefore, it could be suggested that adequate Ca intake is important for balance of the minerals.

The purpose of this study was to estimate usage of vitamin and mineral supplements as over-the-counter (VM-OTC) drugs as well as examine factors associated with VM-OTC usage in Korean adolescents. A total of 1,407 adolescents attending middle or high school in all parts of country were included in the analysis. Prevalence of VM-OTC usage was 56.1%, and it was higher as monthly income, father’s education level, and socioeconomic status of family increased (p<0.001). VMOTC intake was higher in middle school students than in high school students as well as in rural areas or small & mediumsized city residents than big city residents (p<0.01). Subjects mainly received information on VM-OTC mainly from ‘family and relatives’ (46.6%), whereas only 20.3% received information from experts. Subjects took VM-OTC ‘when they are healthy’ (49.1%), ‘when they feel sick’ (17.7%), ‘when they are on a diet’ (17.3%), and ‘when they are stressful’ (15.9%). The effectiveness of taking VM-OTC were mainly ‘fatigue recovery’ (35.0%), ‘health improvement’ (30.6%), and ‘nutritional status improvement’ (13.2%). The most frequently used VM-OTC was vitamin C (49.1%), multi-vitamins (18.6%), multi vitamins & minerals (13.2%), and calcium (9.2%). Among VM-OTC users, only 21.9% replied that they usually check the nutrition facts when they buy products, 62.4% follow the recommended dosage, and 9.7% fully understand the nutrition labels of the products. According to logistic regression analysis, the most influential factor affecting VM-OTC use was parents’ and siblings’ VM-OTC consumption (p<0.001). In addition, school type (middle or high school) (p<0.01), residence (p<0.05), self-concerns about health (p<0.05), father’s education level (p<0.05), and socioeconomic status of family (p<0.05) all influenced VM-OTC use. These results show that VM-OTC use is widespread among adolescents, few users actually check and fully understand the nutrition labels when they purchase VM-OTC, and they are highly dependent on unprofessional advice and information. Therefore, it is necessary to educate adolescents to help them select proper VM-OTC and read nutrition labels.

This experiment was conducted to investigate effect of brine mineral water (BMW) on growth performance and properties of blood in weaning pigs. Treatments allotted were 0% (general water), 2%, 3% and 5% BMW. In growth trial, a total of 36 weaning pig barrows [(Landrace×Yorkshire)×Duroc] weaned after 21 days with an average initial weight of 5.38±0.89 kg were used. Each treatment had 3 replications of 3 pigs per pen in a randomized complete block design. Weaning pigs were investigated for growth performance, complete blood corpuscle count and blood biochemical assay. In results, growth performance of 2% and 3% treatment groups were significantly higher than other groups (p<0.05). In addition, high density lipoprotein cholesterol was significantly (p<0.05) higher in 2% group than other treatment groups. On the other hand, mean corpuscular volume of supplement of BMW treatment groups were significantly (p<0.05) increased than control. Therefore, this study suggests that supplementation of BMW could improve growth performance and level of red blood cell in weaning pigs.

This study was conducted in order to determine the functionality of mineral-rich salt with lower NaCl and higher mineral contents on blood pressure and lipid metabolism in Dahl salt-sensitive rats. A 1% salt solution was administered to five-week-old male Dahl rats– one normal and three salt groups (Purified salt, sun-dried salt, and bamboo salt) for 15 weeks. On the basis of the salt production process, the sun-dried group was classified into two subgroups: SS1 (2-year) and SS2 (>5-year) depending on the storage period of the mineral-rich salt. The relationships between salt intake and changes in blood pressure, serum lipids, and serum mineral concentrations were then examined. The results showed that intake of SS2, which is stored for five years, and BS (bamboo salt) resulted in continuous delay of the increase in blood pressure and inhibited angiotensin–converting enzyme (ACE) activity. In addition, a significant decrease in the triglyceride level in serum lipids of approximately 30% was observed in the SS2 group compared to the PS (purified salt) group. However, all salt intake groups showed an increase in total cholesterol levels compared to the normal group. The results demonstrate that intake of mineral-rich salt is beneficial for the human body and results in reduced blood pressure and triglyceride levels in serum lipids, however, conduct of more research will be needed in order to explore other functions.

The purpose of this study was to investigate the effect of combined exercise training during 12weeks on physical fitness, body composition and bone mineral density in university students. Nineteen male university students who were enrolled of 1st grade at department of physical education in Korea National University of Education were participated in this experiment. Subjects were performed combined exercise training - running, physical training using plyometrics and resistance training - of 5days per week, 70min per 1 time, during 12weeks. The measurement items were health related fitness(muscle strength, muscular endurance, cardiovascular endurance, flexibility), motor skill fitness(power, balance), body composition(% body fat, muscle mass) and bone mineral density(T-score). On the basis of the results analyzed in this study and consideration of many pre studies related it, the result were as follows. First, data analysis of 12 week combined exercise training on health related fitness, post level was revealed more significant increase than pre level at muscle strength(p<.05), muscular endurance(p<.05), cardiovascular endurance(p<.001) and flexibility(p<.05). Second, data analysis of 12 week combined exercise training on motor skill fitness, It was not revealed significant differences between the pre and the post level of the power and balance). Third, data analysis of 12 week combined exercise training on body composition, post level revealed more significant improvement than pre level at muscle mass(p<.05), but It was not revealed significant differences between the pre and the post level of the % body fat. Fourth, data analysis of 12 week combined exercise training on bone mineral density, post level revealed more significant improvement than pre level at T-score(p<.05). In conclusion, It was revealed that 12 week combined exercise training increased physical fitness, and improved body composition and bone mineral density. I hope that more studies will be followed on physical fitness, body composition and bone mineral density on various exercise types, exercise intensity, time and frequency in university students in the future.

PURPOSES : This study is to evaluate ASR(alkali silica reactivity) resistance of ternary blended binder adding ultra fine mineral admixture. METHODS : This study analyzes ASR expansion using ASTM C 1260 and 1567. RESULTS : This study showed that the fineness of mineral admixture had no effect on ASR expansion. The expansion of ternary blended binder(UFFA 20%+FGGBS 10%) were below 0.1%, and this binder met the ASR standard. Also when adding the CSA expansion agent, ASR expansion slightly decreased. The expansion of latex modified mixture increased by 80% comparing plain mixture. CONCLUSIONS : Ternary blended binder met the ASR standard, and this binder is available in concrete bridge deck overlay.

The purpose of this study was to estimate the consumption prevalence of vitamin and mineral supplements as health functional foods (VM-HFF) and to examine the factors associated with VM-HFF consumption behaviors in adolescents. A total of 1,407 adolescents attending middle or high schools from various cities and rural communities in Korea participated in this study. The prevalence of VM-HFF consumption was 41.7%, with boys showing a higher consumption than girls (p<0.01). VM-HFF consumption was higher in families with higher socioeconomic status and for families with parents that exhibited higher concerns about their child's health, growth, and nutritional intake (p<0.001). Most consumers of VM-HFF consumed HFFs 'when healthy' (50.1%), and acquired nutritional information from 'their families & relatives' (50.9%). Most consumers responded that VM-HFF was 'a little effective' (54.3%), followed by 'no obvious effects' (37.1%), and 'very effective' (7.4%). The effectiveness of consuming VM-HFF was mainly for 'fatigue recovery' (39.0%) and 'health improvement' (28.2%). Most consumers purchased HFFs at 'pharmacies & oriental medicine clinics' (53.8%) and at 'health functional food stores' (18.8%). Most consumers 'occasionally' (51.1%) or 'seldom' (27.3%) checked nutrition facts when purchasing, with 58.9% of consumers understanding the nutritional label for 'the most part', but only 8.7% of them understanding it 'very well'. Among the VM-HFF, consumers preferred calcium- and vitamin C-supplements. Consumers' mini-dietary assessment scores were higher than those of non-consumers. The results above showed that VM-HFF consumption was widely spread among adolescents, but few consumers checked and understood the nutrition label when they purchased VM-HFF, and were highly dependent on the advice and information from non-professional nutritionists, such as families & relatives. Therefore, it is necessary to educate adolescents to help them read nutrition labels and select the proper VM-HFF.

부영양호 신갈지의 수질개선을 위하여 이미 개발된 천 연물질 혼합제(PMC)의 수질개선능(WQI)에 수온변화가 어떠한 영향을 미치는지 현장실험을 실시하였다. 실험결 과 식물플랑크톤, BOD, 총인 등은 20􀆆C 이하에서는 70% WQI 이상을 보였으나 25􀆆C 이상에서는 40% WQI 이하로 오히려 감소하였다. 한편 수온의 증가에 따라 남조류에 대해서는 WQI는 감소하였고, 규조류는 수온변화에 상관 없이 90% 이상 높은 WQI를 나타냈으며, 박테리아와 총 질소는 수온과 상관없이 매우 낮은 WQI를 보였다. 이상 의 결과들은 PMC의 수질개선능이 수온과 밀접한 관계를 가지며, 25􀆆C 이상의 고온기에 Microcystis aeruginosa가 우점하는 남조대발생보다 겨울철 저온기에 우점하는 규 조류에 더 효과적일 것으로 판단되었다.

무용수는 공연에 대한 스트레스, 식이 조절, 근골격계 손상 등으로 신체조성의 수준이나 골밀 도에 문제가 있는 것으로 알려지고 있다. 이러한 문제로 인하여 한국무용수가 가지고 있는 신체 조성과 골밀도의 특성을 평가하여 건강상태를 파악하고 그에 따른 관리가 이루어질 필요가 있 다. 따라서, 본 연구는 dual-energy X-ray absorptiometry(DEXA)를 이용하여 한국무용 전공 여대생 10명과 일반 여대생 10명의 신체조성과 골밀도를 비교분석하는데 그 목적이 있다. 신체조성은 측정된 DEXA의 결과에서 body mass index(BMI), 체지방률, 근육량, 제지방량을 사용하였으며 골 밀도는 팔, 다리, 척추, 골반 부위의 골밀도 값을 사용하였다. 연구결과 두 집단 간의 BMI(p=.313), 체지방률(p=.060), 근육량(p=.264), 제지방량(p=.272)의 신 체조성은 통계적으로 유의한 차이가 없었으며, 팔(p=.055), 다리(p=.105), 척추(p=.276), 골반 (p=.126) 부위의 골밀도 또한 통계적으로 유의한 차이가 없었다. 결론적으로 한국무용 전공 여대 생과 일반 여대생의 신체조성 및 골밀도의 차이는 없었다. 이러한 연구결과는 한국무용수에게 한국무용과 식이 섭취 경향이 골밀도와 신체조성을 부정적으로 변화시키지 않았으며 오히려 긍 정적인 영향을 준 가능성이 시사되었다.

This is a basic study of Cheong-song mineral water, which has high contents of Ca and Mg for making noodles as well as for general cooking. The test results of the noodles manufactured and cooked with mineral water were compared to those manufactured and cooked with distilled water and tap water. Noodles manufactured with mineral water showed higher values than others in the cooked weight, volume, water absorption ability, turbidity, lightness (L) and redness (a) on the minus scale. This means that we can manufacture cooked noodles that have high weight, high volume, high water absorption ability and high lightness (L) along with a slight green color with Cheong-song mineral water. Cooked noodles manufactured with mineral water showed significant differences in the cooked weight and volume compared to those manufactured with distilled water. Also, cooked noodles manufactured with mineral water showed significant differences in moisture absorption, turbidity, lightness (L), redness (a) and yellowness (b) compared to those manufactured with distilled water and tap water. Commercial noodles cooked in mineral water showed significant differences in the cooked weight and redness (a) compared to those cooked in distilled water and tap water. Further, commercial noodles cooked in mineral water showed significant differences in weight, volume and brightness (L) compared to those cooked in distilled water.

참죽나무 순채 조기생산을 위하여 하우스와 노지재배에서 생산된 잎의 무기성분을 분석한 결과는 다음과 같다. 참죽나무 순채의 생장속도는 노지에 비하여 하우스 재배에서 다소 빠른 경향이었고, 정아의 경우 초장, 가지수 등 생육과 생체 중, 엽록소 함량 등 품질은 노지와 하우스재배 처리 간 큰 차이가 없었다. 참죽나무 순채의 색도(a값)는 측아보다는 정아에서 적색도가 다소 높았으나 하우스와 노지재배 간에는 차이가 거의 없었다. 참죽나무 정아 및 측아의 pH는 5.7~6.2 정도였고 N 함량은 측아의 평균 500.5mg·kg-1에 비해 정아에서 3.1배 많았고, 노지재배(1,430mg·kg-1)보다는 하우스 재배에서 1.2배 많았다. P, Ca, Mg 및 Fe 함량도 노지재배에 비하여 하우스 재배에서 유의성 있게 높았는데, P는 1.1배, Ca는 1.7배, Mg는 2.0배, Fe는 2.3배 정도 많았으나 K 함량은 하우스 재배의 2.94mg·kg-1에 비하여 노지재배에서 1.6배 많았다. Ascorbic acid 함량은 하우스 측아에서 1,461mg·kg-1으로 가장 높았고 노지 측아, 노지 정아, 하우스 정아 순이었다. 이상의 결과를 종합하면 고품질 참죽나무 순채 생산을 위해서 하우스 재배가 필요한 것으로 생각된다.

식물공장 내 적색광, 청색광, 적청 혼합광, 자외선, 적외선, 형광등의 광원을 달리하여 어린잎 상추의 생육과 무기성분 흡수를 검토하였다. 잎의 형태는 Red 파장에서 초장 및 하배축의 길이신장이 촉진되어 도장하였고 Far red에서는 생장이 불가능하였으나 Red + Blue의 혼합광원에서는 초장이 짧고 엽수가 많고 생체중이 증가하여 상추의 형태 및 발달 차원에서 유의적으로 좋았다. 광질에 따른 어린잎 상추의 색차 및 상대 엽록소 함량을 조사한 결과, 적색도를 나타내는 Hunter a* 값은 Red + Blue 혼합광 및 형광등에서 높았고 적색광 및 자외선에서는 낮게 나타났는데 상대 엽록소 함량을 나타내는 SPAD도 같은 경향이었다. 특히 상대엽록소 함량은 형광등의 10.5에 비해 혼합광에서 1.8배 향상된 적색도를 나타내었다. 광원별 상추의 무기물 함량을 조사한 결과, pH 및 K 함량은 모든 처리에서 차이가 없었으나 N, Ca, Mg, Mn, Fe, Ascorbic acid 함량은 LED 처리구에서 많았고 P 및 Mn 함량은 형광등 처리구에서 많았다. 이상의 결과를 요약하면 단색광에 비하여 Red + Blue 혼합광에서 상추의 생육이 우수하고 무기물 함량이 증대되어 식물공장 내 생산성 향상을 위해서는 혼합광 조절로 상품성 있는 고품질의 상추 생산이 가능 할 것으로 생각되었다.

We investigated the pulpal response to direct pulp capping in rat molar teeth using mineral trioxide aggregate (MTA) and calcium hydroxide (CH). A palatal cavity was prepared in rat maxillary molar teeth. Either MTA or CH was placed on the exposed pulp and all cavities were restored with composite. Rats were sacrificed for histological evaluation after 12 hours and at 2, 7, 14 and 21 days. In both the MTA and CH groups, reparative dentin formation was clearly observed on histology after 14 days. The MTA-capped pulps were found to be mostly free from inflammation, and hard tissue of a tubular consistent barrier was observed. In contrast, in CH-capped teeth, excessive formation of re¬parative dentin toward residual pulp was evident. The pulpal cell response beneath the reparative dentin layer was examined by immunofluorescence using antibodies against DSP. After 2 days, a few DSP immunopositive cells, most of which showed a cuboidal shape, appeared beneath the predentin layer. At 7 days, DSP-immunopositive cells with columnar odontoblast-like cells were seen beneath the newly formed hard tissues. At 14 and 21 days, DSP was more abundant in the vicinity of the odontoblastic process along the dentinal tubules than in the mineralized reparative dentin. The CH group showed strong expression patterns in terms of DSP immunoreactivity. Our results thus indicate that MTA may be a more effective pulp capping material as it induces the differentiation of odontoblast-like cells and the formation of reparative dentin without the loss of residual pulp functions.