Chrysanthemum white rust, caused by Puccinia horiana, is one of the most destructive fungal diseases in chrysanthemum cultivation worldwide. For increasing efficiency of resistant breeding, molecular markers linked to chrysanthemum white rust resistance gene were developed in pseudo F1 cross population between ‘Puma White’ as susceptible and ‘Dancer’ as resistant using bulked segregant analysis (BSA). Of 280 RAPD primers (Operon 10 mer), 18 primers found to be polymorphic. After screening of these primers in 20 individual lines, only OPI-13520 was selected as closely linked marker to white rust disease resistance. Based on correspondence between phenotypic resistant level and marker in 187 segregation population, the genetic distance between white rust resistance gene and OPI-13520 marker assumed to be 3.8 cM. For OPI-13520 marker conversion into sequence characterized amplified region (SCAR) marker, the amplified fragment of OPI-13520 was purified, cloned and sequenced. Based on the DNA sequence of OPI-13520, SCAR maker was generated and verified in 20 individual lines used in BSA-RAPD.The results showed SCAR marker could be used to identify white rust resistance in chrysanthemum.

• Sang Kun Park(Floricultural Research Division, National Institute of Horticultural and Herbal Science) Corresponding Author
• Jae-A Jung(Floricultural Research Division, National Institute of Horticultural and Herbal Science)
• Young Soon Kwon(Floricultural Research Division, National Institute of Horticultural and Herbal Science)
• Bong Sik Yoo(Floricultural Research Division, National Institute of Horticultural and Herbal Science)
• Kyeong Seong Cheon(Floricultural Research Division, National Institute of Horticultural and Herbal Science)
• Hak-ki Shin(Floricultural Research Division, National Institute of Horticultural and Herbal Science)