KOREASCHOLAR

We evaluated the protective effects of cricket methanol extract (CME) on ultra-violet B (UVB)-induced photoaging in human skin fibroblasts. The fibroblast cells were treated with 10, 50, and 100 μg/mL of CME for 24 h, and then exposed to UVB (30 mJ/cm2). CME showed a dose-dependent cytoprotective effect without any observable cytotoxicity. CME reduced UVB-induced production of reactive oxygen species (ROS) by 34.4, 34.9, 40.6% at concentrations of 10, 50, 100 μg/mL respectively. CME inhibited the release of matrix metalloproteinase (MMP) 1 and 3. Furthermore, CME also reduced UVB-induced collagen degradation in the fibroblast cells. Taken together, our data suggests that CME has a significant protective effect on UVB-induced photoaging of the skin. This benefit occurs through multiple mechanisms. The results also suggest a potential role for CME as an ingredient in anti-photoaging cosmetic products in the future.

Abstract
I. 서 론
II. 연구 내용 및 방법
    1. 재료 및 시료의 추출
    2. 세포배양 및 세포 독성 실험
    3. UVB 조사
    4. 활성산소종(ROS) 생성 측정
    5. MMP-1, MMP-3 및 수용성 콜라겐 생성 측정
    6. 통계처리
III. 결과 및 고찰
    1. 귀뚜라미 메탄올 추출물의 세포 독성 및 피부세포 보호효과
    2. ROS 생성에 대한 귀뚜라미 메탄올 추출물의 억제효과
    3. MMP-1, MMP-3 및 수용성 콜라겐 생성에 대한 귀뚜라미메탄올 추출물의 효과
IV. 요약 및 결론
References

• 정택영(충북대학교 식품생명공학과) | Taekyoung Jeong (Department of Food Science and Biotechnology, Chungbuk National University)
• 유명남(충북대학교 식품생명공학과) | Myeongnam Yu (Department of Food Science and Biotechnology, Chungbuk National University)
• 허희진(충북대학교 식품생명공학과) | Huijin Heo (Department of Food Science and Biotechnology, Chungbuk National University)
• 양진우(국립식량과학원 밀연구팀) | Jinwoo Yang (Wheat research team, National Institute of Crop Science)
• 정헌상(충북대학교 식품생명공학과) | Heonsang Jeong (Department of Food Science and Biotechnology, Chungbuk National University)
• 이준수(충북대학교 식품생명공학과) | Junsoo Lee (Department of Food Science and Biotechnology, Chungbuk National University) Corresponding author