KOREASCHOLAR

산화적 스트레스에 대한 여주(Momordica charantia Linn.)잎의 항산화 활성 및 간세포 보호능 Antioxidant and Hepatoprotective Activities of Bitter Melon (Momordica charantia Linn.) Leaves against Oxidative Stress

전아영, 천원영, 윤지민, 김대중, 김영화
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  • URLhttps://db.koreascholar.com/Article/Detail/404809
韓國食生活文化學會誌 (한국식생활문화학회지)
제35권 제6호 (2020.12)
pp.597-604
한국식생활문화학회 (The Korean Society Of Food Culture)
초록

This study examined the bioactive compound content and the antioxidant activities of bitter melon (Momordica charantia Linn.) leaves. The content of vitamin C, beta-carotene, and total carotenoids was 69.77, 45.68, and 65.08 mg/100 g, respectively. To investigate the antioxidant capacity, bitter melon leaves were extracted using various concentrations of ethanol (60, 80, or 100%). Highest content of total polyphenols (18.07 mg gallic acid equivalent/g) and flavonoids (4.53 mg cathechin equivalent/g) was found in the 100% ethanolic extract of the leaves (E100). Also, the E100 extract showed the highest levels of 2,2'azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and α-α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging activities. Reducing power was also the highest (39.21 mg Trolox equivalent/g) in E100 extract. The E100 extract effectively inhibited lipid peroxidation by 91.45% compared to the control group. Also, the E100 extract showed a cytoprotective effect against oxidative stress in HepG2 cells and decreased the generation of intracellular reactive oxygen species. These results suggest that bitter melon leaves could be regarded as a potential source of natural antioxidants.

키워드
Bitter melon leafradical scavenging activityhepatoprotective effectintracellular reactive oxygen speciesantioxidant
목차
Abstract
I. 서 론
II. 연구 내용 및 방법
    1. 재료 및 시약
    2. 여주잎 ethanol 추출물 제조
    3. Vitamin C 분석
    4. Carotenoid 및 beta-carotene 분석
    5. 총 폴리페놀 및 플라보노이드 측정
    6. 라디컬 소거능 측정
    7. 환원력 측정
    8. 지질 과산화 저해능 측정
    9. 시료 독성 및 간세포 보호능 측정
    10. 세포 내 활성산소종 측정
    11. 통계분석
III. 결과 및 고찰
    1. 여주잎의 기능성 성분 분석
    2. 라디컬 소거능 및 지질 과산화 억제능 측정
    3. 산화적 스트레스에 대한 간세포 보호 작용
IV. 요약 및 결론
감사의 글
Conflict of Interest
References
저자
  • 전아영(경성대학교 식품생명공학과) | Ahyeong Jeon (Department of Food Science and Biotechnology, Kyungsung University)
  • 천원영(경성대학교 식품생명공학과) | Wonyoung Cheon (Department of Food Science and Biotechnology, Kyungsung University)
  • 윤지민(경성대학교 식품생명공학과) | Jimin Yoon (Department of Food Science and Biotechnology, Kyungsung University)
  • 김대중(국립농산물품질관리원 시험연구소) | Dae-Jung Kim (Experiment research institute, National Agricultural Products Quality Management Service)
  • 김영화(경성대학교 식품응용공학부) | Younghwa Kim (School of Food Biotechnology and Nutrition, Kyungsung University) Corresponding author