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몽골과 미얀마 식물 14종의 3T3-L1 및 HepG2 세포에서 지질 축적 억제효과 Inhibitory Effects of 14 Plants from Mongolia and Myanmar on Lipid Accumulation in 3T3-L1 and HepG2 Cells

김숙진, 김건희
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  • URLhttps://db.koreascholar.com/Article/Detail/405720
韓國食生活文化學會誌 (한국식생활문화학회지)
제36권 제1호 (2021.02)
pp.130-142
한국식생활문화학회 (The Korean Society Of Food Culture)
초록

This study examined the antioxidative and lipid accumulation inhibitory effects of 14 plants from Mongolia and Myanmar on 3T3-L1 and HepG2 cells. The total phenolic and flavonoid contents (TPC and TFC) of 14 plant extracts were measured, and the antioxidative activities were analyzed using DPPH, ABTS, FRAP, and ORAC. After measuring the pancreatic lipase levels and performing the thiobarbituric acid assay, the degree of lipid accumulation was determined by lipid (Oil Red O) staining and triglyceride assay in 3T3-L1 and HepG2 cells. M. paniculate (259.43 mgGAE/g) and C. benghalensis (130.78 mgNAE/g) had the highest TPC and TFC, respectively, among the 14 plants. R. acicularis Lindl. had the highest antioxidant activity in DPPH. The ABTS, FRAP, and ORAC results showed that the antioxidant activity of 11 species was higher than that of the positive control. The pancreatic lipase inhibitory effect of C. angustifolium Scop. was reduced to 23.65% at 0.1 mg/mL, and the level of lipid peroxidation of C. abrorescens Lam. was 0.63 nmol/mg. Five selected plants inhibited the lipid accumulation and triglyceride content, respectively, in 3T3-L1 and HepG2 cells. These results provide scientific evidence for developing functional foods using 14 plants from Mongolia and Myanmar, which have antioxidant activities and lipid accumulation reduction effects.

키워드
Mongoliamyanmarantioxidantlipid accumulation3T3-L1hepG2
목차
Abstract
I. 서 론
II. 연구내용 및 방법
    1. 실험재료
    2. 시료 준비
    3. 총 폴리페놀 및 플라보노이드 함량
    4. DPPH radical 소거능 측정
    5. ABTS radical 소거능 측정
    6. FRAP assay
    7. ORAC assay
    8. Pancreatic lipase 저해활성 측정
    9. TBA assay
    10. 3T3-L1 및 HepG2 세포 배양 및 분화
    11. MTT assay
    12. Oil red O staining
    13. Triglyceride assay
    14. 통계처리
III. 결과 및 고찰
    1. 몽골 및 미얀마 식물의 총 폴리페놀 및 플라보노이드 함량
    2. 몽골 및 미얀마 식물의 항산화 활성
    3. 몽골 및 미얀마 식물의 Pancreatic lipase 저해 활성
    4. 몽골 및 미얀마 식물의 지질 과산화물 함량 억제 활성
    5. 몽골 및 미얀마 식물의 세포 독성 평가
    6. 몽골 및 미얀마 식물의 3T3-L1 세포에서 지질 축적 및 중성지방 함량 억제 효과
    7. 몽골 및 미얀마 식물의 HepG2 세포에서 지질 축적 및 중성지방 함량 억제 효과
IV. 요약 및 결론
References
저자
  • 김숙진(덕성여자대학교 바이오헬스융합학과) | SukJin Kim (Department of Bio-Health Convergence Major, Duksung Women’s University)
  • 김건희(덕성여자대학교 바이오헬스융합학과) | Gun-Hee Kim (Department of Bio-Health Convergence Major, Duksung Women’s University) Corresponding author