감태나무 뿌리 추출물에 의한 대장암세포의 성장억제 및 세포사멸유도 Anti-proliferative and Apoptotic Activity of Extracts of Lindera glauca Blume root in Human HCT116 Colorectal Cancer Cells
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/407038
pp.235-245
Lindera glauca Blume has been used in Korean traditional medicine to treat the symptoms of paralysis, abdominal pain, speech disorders, extravasations, contusions, and pain caused by rheumatoid arthritis. We investigated the effect of L. glauca Blume extracts on the proliferation of colorectal cancer cells in vitro using HCT116 human colorectal cancer cell lines. We also investigated its mechanism of action. For this purpose, we used the MTT assay, western blotting, DNA fragmentation analysis, and flow cytometry. HCT116 cells were cultured in several concentrations of ethanol extracts of L. glauca Blume root (0, 50, 100 μg/mL). In this study, colon cancer cell growth was inhibited by L. glauca Blume root extract in a dose-dependent manner. It was associated with induction of apoptosis as assessed by nuclear fragmentation and cell cycle analysis. Apoptosis was assessed using western blotting for TNF-α, IL-6, NF-κB, Caspase-3, PARP, Bax, Bcl-2, and SIRT1. The extract also dose-dependently upregulated the expression Bax, the pro-apoptotic gene and downregulated the expression of the anti-apoptotic gene Bcl-2. Furthermore, the extract enhanced Caspase-3 activity in a dose-dependent manner. Our findings provide evidence that L. glauca Blume extract may mediate its anti-proliferative effect via the modulation of apoptosis.
I. 서 론
II. 연구 내용 및 방법
1. 실험재료
2. 추출물의 제조
3. 세포배양
4. 세포 증식 억제능 측정
5. Western blot analysis
6. DNA 분절 현상 측정
7. 세포주기 측정
8. 통계처리
III. 결과 및 고찰
1. 감태나무 뿌리 추출물 처리에 의한 HCT116 암세포 성장억제효과
2. 감태나무 뿌리 추출물 처리에 의한 DNA 분절 유도효과
3. 감태나무 뿌리 추출물 처리에 의해 Caspase의 활성 및PARP분절
4. HCT116 세포의 Bcl-2와 Bax의 발현에 감태나무 뿌리 추출물 처리가 미치는 영향
5. 감태나무 뿌리 추출물이 세포주기에 미치는 영향
6. 염증성 사이토카인과 NF-κB 유전자 발현에 감태나무 뿌리추출물의 영향
7. 감태나무 뿌리 추출물 처리가 HCT116 세포의 SIRT1 발현에 미치는 영향
IV. 요약 및 결론
References