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LPS로 자극한 microglia BV2 cell에서 Cyrtomium fortunei J.Sm. 추출물의 항염증 효과 Anti-inflammation Effect of Cyrtomium fortunei J.Sm. Extracts in Lipopolysaccharides-induced Microglia BV2 Cell

최지원, 김신태, 최상윤, 최인욱, 허진영
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  • URLhttps://db.koreascholar.com/Article/Detail/423406
韓國食生活文化學會誌 (한국식생활문화학회지)
제38권 제3호 (2023.06)
pp.176-183
한국식생활문화학회 (The Korean Society Of Food Culture)
초록

In this study, we investigated the effect of the extracts of Cyrtomium fortunei J.Sm. (CFJ) on lipopolysaccharide (LPS) induced inflammation in mouse BV-2 microglial cells. Nitric oxide (NO) production and cell viability were measured using the Griess reagent and the (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) assay. Inflammatory cytokines were detected by quantitative polymerase chain reaction (qPCR) in BV-2 microglial cells with and without CFJ extracts. Subsequently, mitogen-activated protein kinases (MAPKs) and antioxidant markers were assessed by western blot analysis. It was found that the CFJ extract significantly decreased the production of pro-inflammatory cytokines (interleukin [IL]-6, tumor necrosis factor [TNF]-, and IL-1) and NO in BV-2 microglial cells that were stimulated with LPS. In addition, the expression levels of the phosphorylation of the MAPK family (p38, c-Jun N-terminal kinases [JNK], and extracellularsignal regulated kinase [ERK]) were reduced by CFJ. Also, treatment with CFJ significantly increased the activities of superoxide dismutase type 1(SOD1) and Catalase in BV-2 microglial cells. Our results indicate that CFJ has a potent suppressive effect on the pro-inflammatory responses of activated BV-2 microglia. Therefore, CFJ has the potential to be an effective treatment for neurodegenerative diseases, as it can inhibit the production of inflammatory mediators in activated BV-2 microglial cells.

목차
I. 서 론
II. 연구 내용 및 방법
    1. 재료 및 방법
    2. Cyrtomium fortunei J.Sm. 추출물 (CFJ) 제조
    3. 세포배양
    4. 세포 생존율 측정
    5. Nitric Oxide (NO) 생성 저해 효과
    6. Western blot을 통한 단백질 발현
    7. RT-PCR을 통한 유전자 발현
    8. 통계분석
III. 결과 및 고찰
    1. CFJ의 세포 생존률과 NO 생성 억제 효능
    2. CFJ의 염증성 매개 인자인 iNOS, COX-2 생성 억제 효능
    3. CFJ의 염증성 cytokine 생성 억제 효능
    4. CFJ의 MAPKs 활성화 억제 효능 검증
    5. CFJ의 산화적 스트레스 억제효능
IV. 요약 및 결론
감사의 글
Conflict of Interest
저자
  • 최지원(한국식품연구원) | Jiwon Choi (Korea Food Research Institute)
  • 김신태(한국식품연구원) | Shintae Kim (Korea Food Research Institute)
  • 최상윤(한국식품연구원) | Sang Yoon Choi (Korea Food Research Institute)
  • 최인욱(한국식품연구원) | Inwook Choi (Korea Food Research Institute)
  • 허진영(한국식품연구원, 한국과학기술연합대학원 대학교) | Jinyoung Hur (Korea Food Research Institute, University of Science Technology (UST)) Corresponding Author