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사삼 및 그 추출물에 대한 지표물질 분석법 확립 및 안전성 조사 Establishment of Analytical Methods for Marker Compounds and Safety Evaluation of Adenophora triphylla and its extract

편지예, 김윤정, 유영은, 정철종, 이도상, 나임정, 김영화
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  • URLhttps://db.koreascholar.com/Article/Detail/446638
韓國食生活文化學會誌 (한국식생활문화학회지)
제40권 제5호 (2025.10)
pp.200-209
한국식생활문화학회 (The Korean Society Of Food Culture)
초록

This study aimed to establish an analytical method for the use of Adenophora triphylla and its extract using citric acid as a marker compound. The analysis of A. triphylla and its extract revealed citric acid contents of 671.993 mg/100 g and 433.218 mg/100 g, respectively. The analytical method was validated according to the Association of Official Analytical Chemists (AOAC) guidelines, and the calibration curve for citric acid standard solution exhibited excellent linearity (R2>0.999). The intra-day and inter-day precision showed low relative standard deviations of 0.564 and 0.809%, respectively. Furthermore, the recovery rate for citric acid ranged from 96.578 to 100.525%, fulfilling the AOAC’s acceptable range, indicating high accuracy. The detection limit for citric acid in this method was 0.002 mg/100 g, and the quantification limit was 0.005 mg/100 g, demonstrating high sensitivity. Additionally, an investigation of potentially harmful compounds in A. triphylla and its extract revealed five compounds that were predicted to be harmful according to the RISCTOX database. However, their actual concentrations were far below the lethal dose 50 (LD50) values, suggesting they are not harmful to the humans. These findings provide foundational data for the quality control of A. triphylla as a food ingredient.

키워드
Adenophora triphyllamarker compoundanalytical methodsafety evaluationmethod validation
목차
Abstract
I. 서 론
II. 연구 내용 및 방법
    1. 실험재료 및 시약
    2. 지표물질 선정 및 추출, 분석
    3. 분석법 검증
    4. 사삼 및 사삼 추출물의 안전성 조사
    5. 잠재적 유해물질 정량분석
    6. 통계분석
III. 결과 및 고찰
    1. 사삼의 지표물질 선정 및 분석법 검증
    2. 사삼과 사삼 추출물의 citric acid 함량
    3. 사삼의 잠재적 위험 요소 및 함량 분석
IV. 결론 및 요약
저자정보
감사의 글
Conflict of Interest
References
저자
  • 편지예(경성대학교 식품생명공학과 BB21plus 프로젝트 팀) | Jiye Pyeon (Department of Food Science and Biotechnology, BB21plus project team, Kyungsung University)
  • 김윤정(경성대학교 식품생명공학과 BB21plus 프로젝트 팀) | Yoonjeong Kim (Department of Food Science and Biotechnology, BB21plus project team, Kyungsung University)
  • 김영화(경성대학교 식품생명공학과 BB21plus 프로젝트 팀) | Younghwa Kim (Department of Food Science and Biotechnology, BB21plus project team, Kyungsung University) Corresponding author
  • 유영은((주)옥천당 중앙연구소) | Yeong-Eun Yu (Central Research Center, Okchundang Inc.)
  • 정철종((주)옥천당 중앙연구소) | Cheol-Jong Jung (Central Research Center, Okchundang Inc.)
  • 이도상((주)애터미오롯 R&D 센터) | Do Sang Lee (Atomy R&D Center)
  • 나임정((주)애터미오롯 R&D 센터) | Im-Joung La (Atomy R&D Center)