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털두꺼비하늘소(Moechotype diphysis)로부터 Xylanase를 생산하는 Paenibacillus sp. HY-8 균주의 분리 및 특성 KCI 등재

Isolation and Characterization of Xylanase-producing Paenibacillus sp. HY-8 from Moechotype diphysis

허선연, 오현우, 박두상, 김향미, 배경숙, 박호용
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한국응용곤충학회지 (Korean Journal of Applied Entomology)
한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

From the course of screening of useful xylanase producing microorganism from a phytophagous longicorn beetle, we isolated an extra-cellular xylanase producing strain, Paenibacillus sp. HY-8 from the intestine of Moechotypa diphysis adult. On the basis of morphological, biochemical and phylogenetic studies of the new isolate was identified as a Paenibacillus species. Production of xylanase in this strain was strongly induced by adding xylan to the growth medium and repressed by glucose or xylose. The highest xylanase production was attained in the M9 media containing 1% yeast extract and 0.5% birchwood xylan when cultured at 25℃ for 24 hrs. HY-8 producing xylanase showed superior hydrolytic activities against various plant source feedstuff than control xylanase produced by Tricoderma sp. at pH 6.0.

키워드
Paenibacillus sp. HY-8Moechotypa diphysisXylanaseInsect symbiotic microorganism털두꺼비하늘소자일라나제곤충공생미생물
저자
  • 허선연
  • 오현우
  • 박두상
  • 김향미
  • 배경숙
  • 박호용