Our environment is faced with serious problems related to the air pollution from automobiles in these days. In particular, the exhaust emissions of diesel engines are recognized as main causes of the air pollution. DI diesel engine is widely used for the sake of minimization on exhaust emission. Because biodiesel fuel is a renewable and alternative fuel for diesel engine, its usability is expanded. The smoke emission of esterfied rapeseed oil is reduced remarkably in comparison with commercial gas oil, that is, it was reduced approximately 44.5% at 1500rpm. But, brake specific energy consumption showed very slight differences. It was concluded that esterfied rapeseed oil can utilize effectively as an alternative and renew-able fuel for diesel engine.