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Disc Electrophoresis에 의한 면화의 Genome과 종간관계 분석 KCI 등재

Analysis of Genome and Species Relationships in Cotton by Disc Electrophoresis

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한국작물학회지 (Korean Journal of Crop Science)
한국작물학회 (Korean Society Of Crop Science)
초록

Protein spectra from 6 species of Gossypium were obtained by disc electraphoresis of seed extracts. Protein extracts were made by soaking 0.5g. of seed in 15ml of Tris-glycine buffer for 24 hours. Gels 24 hours. Gels were stained in 0.5% Amido Black solution for 1 hour, and destained in 7% acetic acid for 72 hours. Nine to 15 bands were visible in each gel. Homologies of Protein bands among the species were determined by migration velocity. Evidences obtained from electrophoretic separation of seed Protein were consistent with those from genetic, cytological, morphological and Phenogenetic methods regarding the origin of New World cultivated cottons. Possibility, however, does not exist to exclude Gossypium herbaceum from one of the Progenitors of New World cultivated cottons from electrophoretic evidences alone.

저자
  • 崔鍾烈(강원대학 농학부) | 최종열
  • 張炳晧(강원대학 농학부) | 장병호