생쥐 정자의 수정능력획득과 첨체반응에 작용하는 /H antiporter의 역할을 조사하고자 하였다. /H antiporter를 특이적으로 억제하는 dimethyl amiloride는 정자의 자발적인 첨체반응을 농도 의존적으로 억제한 반면 난포액 및 calcium ionophore인 A23l87에 의해 유도된 첨체반응은 억제하지 못하였다. 이러한 결과는 정자내 /H antiporter에 의한 1가이온의 출입과 이에 따른 세포질내 pH 조절이 정자의 수정능

The possible role of Na/H antiporter in both the capacitation and the acrosome reaction (AR) was examined in mouse epididymal spermatozoa. Spontaneous acrosome reaction was inhibited by dimethyl amiloride (DMA), a specific inhibitor of Na/H antiporter, with dose dependent manner. Follicular fluid- or A23l 87-induced acrosome reaction was not inhibited by DMA. It suggests that change in pH by monovalent cation transport through the Na/H antiporter is possibly engaged in the capacitation and that agonist- as well as A23l87-induced AR in capacitated sperm might be independent from the Na/H antiporter. Conclusively, changes in pH through the Na/H antiporter might be important for sperm capacitation and it virtually occurs upstream of the influx which precedes the acrosome reaction in mouse epididymal spermatozoa.pididymal spermatozoa.

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