Varietal Responses of Soybean Germination and Seedling Elongation to Temperature and Polyethylene Glycol Solution
온도(15℃ 및 30℃ )와 PEG용액(O, 20, 30 및 35g / 100g H2O ) 농도를 달리여 대두품종(방사콩, Hill 콩, 팔달콩, 두엽콩, 백운콩, 광교, 장엽콩 및 황금콩)의 발아와 묘신장 반응을 측정하였다. PEG 용액 농도의 조절은 상온에서 0.0, -0.5, -1.1 및 -1.5 MPa 수분포텐셜을 기초로 하였다. 시료의 채취는 15℃ 에서 3, 6, 9 및 12일 간격으로, 30℃ 에서는 1, 2, 3 및 4일간격으로 실시하였다. 품종당 15립의 종자를 plastic petridish 안의 Whatman No.1(9cm) 여지위에 자동 주사기로 0.2% 0.2% Thiram을 첨가한 농도별로 PEG용액을 15ml 씩 조절하였다. Plastic petri-야노를 Cap으로 대하여 15℃ 및 30℃ 항온의 무광조건하에서 발아시켰다. 8개 품종의 묘수분함량과 묘장이 15℃ 및 30℃ 에서 공히 PEG용액 농도가 증가할수록 감소되었다. 묘수분함량과 묘장의 품종간차이가 두 온도에서 공히 발견되었다. 대립중이 소립중보다 많은 양의 수분을 흡수하였으나 수분함량(%)은 소립중이 높았다. Hill 콩과 팔달콩은 두 온도에서 공히 PEG 용액농도 20에서 30g/100g H2O 까지 타품종들에 비교하여 큰 묘장을 보였다. 본 시험의 연구결과에서 PEG용액 농도에 대한 대두발아반응 시험으로 조기시 출현 증진을 위한 대두품종 선발 가능성이 있음을 보였다.
Germination and seedling elongation of soybeans [Glycine max. (L.) Merr., cults. Bangsakong, Hill, Paldalkong, Danyupkong, Baegwun-kong. Kwangkyokong, Changyupkong and Hwangkeumkong] were measured at two temperatures (15 and 30~circC ) and four polyethylene glycol 10,000 (PEG) solutions (0, 20, 30 and 35g/100g-H2 O). Adjustments of PEG solution were based on water potentials of 0.0, -0.5, -1.1 and -1.5 MPa at room temperature. Observations were made at 3, 6, 9 and 12 days for 15~circC and 1, 2, 3 and 4 days for 30~circC . Fifteen seeds of each cultivar were placed on Whatman No. 1 (9cm) filter paper in plastic pertridishes, and adjusted to 15 ml of the proper PEG solution supplemented with 0.2 percent thiram using automatic syringe. The dishes were covered with cap. The seeds were germinated at a continuous temperature of 15 or 30~circC under dark conditions for programmed period. Seedling moisture content and seedling length of eight soybean cultivars decreased as PEG concentration increased both at 15 and 30~circC . Cultivar differences in seedling moisture content and seedling length were found among eight soybean cultivars at temperatures of 15 and 30~circC . Larger sized cultivar absorbed more moisture than samller sized cultivar. However, reverse results were obtained on the seedling moisture content of each of eight soybean cultivars. Cultiver Hill and Paldalkong showed greater seedling length than the other six cultivars from 20 to 30g/100g water of PEG concentrations both at 15 and 30~circC . The results of this study indicated that germination test of soybean seeds in aqueous solutions of PEG has potential for screening soybean cultivars for improved emergence during moisture stress.