논문 상세보기
pp.74-78
밀 약배양에 있어서 Callus 유기, 식물체분화 및 분화식물체의 염색체 변이에 미치는 돌연변이 유기제의 처리 효과를 검토코자 감수 분열기 1 핵기 이삭에 X-ray는 4수준( 100, 200, 500, 1,000 rad) Ethyle Methane Sulphonate 는 3수준 ( 0.1, 0.2, 0.3M)으로 각각 처리하여 Cl7배지에서 callus를 유기시키고 1/2 MS 배지에서 식물체를 분화시켰다. 그 결과 callus 유기는 억제되었으나 식물체분화는 다소 높았으며 특히 약치상 후 61～80일째 장기치상된 약의 callus에서 식물체분화율이 높았다. 분화식물체의 염색체 변이는 반수체(n=3 x=21) 발생율이 증가되었고 n=20, n=21개의 염색체를 갖는 이수체가 발생되어 염색체 변이가 컸다.다.
This experiment was conducted to know the effects of mutagen treatments on callus induction, plant regeneration and their ploidy in the anther culture of wheat. The winter wheat cultivars, 'apos;and 'apos;Wonkwang'apos;, were treated at the mid or late-uninucreate stage under 4 different doses (100, 200, 500 and 1,000 rad.) of X-ray and 3 different levels(0.1, 0.2 and 0.3 mole) of Ethyl Methane Sulphonate. The anthers treated were set on the C~ 17/medium for callus induction, and callus induced was transfered to 1/2 MS medium for plant regeneration. The mutagen treatments inhibited the callus induction but increased the plant regeneration in the callus which were induced from the anther set on the medium for the long time of 60 to 80 days. Also, the chromosome number to the regenerated plant varied largely by increasing of haploid plants(n=3x=21) and by occurring of aneuploidy having n=20 and n=22 of chromosome number.aried largely by increasing of haploid plants(n=3x=21) and by occurring of aneuploidy having n=20 and n=22 of chromosome number.
