논문 상세보기
pp.476-486
1. 옥수수의 자식계 9계통에 화학적 돌연변이 유기제를 처리하여 얻어진 M1 , 식물들은 형태적으로 왜성화, 백색화등 다양한 변이체로 나타났고 생식기관의 변이는 자성개체, 웅성개체, 자웅동화, 다수성들을 보였고 부임개체들이 증가하였다. 2. mutagen의 종자침지처리후 이삭에 수분방지대를 씌웠을때는 3.9-11.2%의 이삭착립율이 나타났으나 이삭당 성숙립수는 3.8-4.3게에 불과하였다. 한편 mutagen의 이삭주입처리에서는 5.1-10%의 착립이삭율과 이삭당 성숙립수는 1.7-6.3이었고 자연수분후 4시간만에 웅사를 절단하였던 이삭들은 착립이삭수가 27%로 증가하였다. 3. Mutagen처리와 수분방지처리에서 형성된 종자들의 조직학적 관찰은 aposporous apomictic reproduction이나 adventitious embryony의 가능성을 보여주었고 M2식물의 근단염색체를 조사하였을때 모두 2배체로서 확인되었다. 4. 무배합생식성 종자에서 출현된 식물들은 초장의 변이계수가 4-6%로 비교적 낮았고 외부형태상의 균일성을 보여주었다. 그러나 이들 무배합생식성과 결부된 표식형질을 발견할수는 없었다.
M1 plants which were produced from seed soaking in chemical mutagen, EMS or NaN3 , appeared wide morphorogical variations such as dwarf, albino, twisted leaf, white streaked leaf, and purpled stem. In mutants of reproductive organs, there were monoecious plants such as female-flower plant and male-flower plant, multiple spikes, and steriled plants among M1 plants. Also, barren stalk was increased significantly in M1 plants. Ear bagging at ear initiation stage prevented seed set on cob in normal plants. In spite of ear bagging, M1 plants which had cobs with seed set was 3.9-11.2% of stalks developed from seeds soaking with mutagens, but only three or four kernels could be matured on a cob. Ear bagging after mutagen injection into initiating ear produced 5.1-10% in cobs with seed set, but only 1.7-6.3 kernels could be matured. Cobs removed silk at four hours after artificial pollination increased the rate of cobs with seed set to 27%. Microscopic observation confirmed that ontogeny of kernels matured from ear bagging and mutagen treatment would be both adventitious and diplosporous apomictic reproduction. Chromosome set of M2 seedling was found to be diploid type in chromosomal counting of root tip. As M2 plants showed an uniform appearence within each lines and their CV of plant height were ranged 4-6% in each lines, we concluded that they were apomictic progeny. But we could not find any marker traits combined with apomixis.
