Insulin과 tumor necrosis factor alpha(TNF-)에 의한 초기 배아 발생의 조절기작을 알아보고자 생쥐의 상실배를 대상으로 이들이 첨가된 배양액에서 형태발생, 세포증식을 조사하고, 포배에서 mitogen activated protein kinase(MAPK, ERK1/2)의 활성 변화에 미치는 영향을 조사하였다. Insulin은 상실배의 체외발생 및 포배내 할구 수를 대조군에 비해 유의하게 증가시켰으며, TNF-는 발생율을 유의

Present study was aimed to verify the role of insulin and TNF- in development of preimplantation embryos. Mouse morula were cultured for 40 hr in the presence or absence of insulin(400 ng/ml) and TNF- (50 ng/ml). The morphological development, cell number of blastomeres per blastocyst, and mitogen activated protein kinase(MAPK) activity were examined. The developmental rate and cell number per embryo were the highest in insulin treatment group and the lowest in TNF- treatment group. There was no significant difference in developmental rate between control and insulin plus TNF- group. Taken together, it suggested that TNF- impaired embryonic development and that insulin rescued developmental impairment imposed by TNF-. In blastocysts, insulin treatment significantly increased MAPK activity. TNF- decreased the MAPK activity in a concentration-dependent manner. In the TNF-(50 ng/ml) -primed embryos, activation of MAPK by insulin was attenuated. In conclusion, these results suggest that there was a cross talk between insulin and TNF- by means of activation of MAPK in preimplantation embryos and that insulin might rescue damage of embryos exposed to TNF-.

• 계명찬
• 한현주
• 최진국