본 연구는 소의 미성숙 난포란의 체외성숙시 의 첨가가 체외성숙, 체외수정 후 웅성전핵의 형성 및 세포질 내의 GSH 수준에 미치는 영향을 알아보고자 실시하였다. 체외성숙시 과 의 를 첨가한 경우 대조구에 비하여 성숙율이 증가하는 것으로 나타났으며(p<0.05), 모든 실험구에 있어서 12시간 체외성숙보다 24시간 체외성숙에서 높은 성숙율을 나타냈다(p<0.05). 체외수정 후 웅성전핵 형성에 있어서는 와 농도의 첨가구에서 대조구에 비하여 높게 나타났으나

Experiments were conducted to determine the effects of beta-mercaptoethanol() supplements to the maturation medium on in vitro fertilization(IVF) and intracellular glutathione(GSH) concentration. Bovine cumulus-intact oocytes were matured in TCM-199 medium containing FBS, hormonal supplements, and (0, 25 and ) for 12h and 24 h. After culture, cumulus-free matured oocytes were co-incubated with frozen-thawed spermatozoa for 24h. Maturation rate increased(p<0.05) in treatment group, but no significant differences among treatment groups. Also, increases(p<0.05) in intracellular GSH concentration before and after fertilization were observed in supplements to the maturation medium. Male pronuclear formations after IVF was increased(p<0.05) in treatment group, but no significant difference among treatment groups. In conclusion, supplementing into the maturation medium increased maturation rates, fertilization rates, and intracellular GSH concentrations.

• 오신애
• 김창근
• 정영채
• 방명걸