Rice and weed interference in the paddy field caused by resource competition and allelopathy. Evaluation method of active weed suppressive behavior of rice to weed was developed by eliminating light competition at soil condition. Twenty eight days old rice seedlings (6-7 leaf stage) which was grown at saturated, no drainage pot were clipped above 3~~4cm from the soil surface. Weeds seeded around clipped rice stem, named ratoon seeding screening method, showed varietal suppressive difference to Echinochloa crus-galli, Echinochloa crusgalli var. praticola and Monochoria vaginalis. Potential allelopathic rice varieties, Sathi, AC1423 and PI312777 showed better suppressive activity to weed seedling growth than Nonganbyeo and Keumobyeo. Weed suppression of one plant of rice cultivars could be evaluated by the cell size of 2.5~times2.5cm at rice clipping of seedling 29 days after rice seeding.