This study was performed to comprehend the plasma proteins expressed specifically during early pregnancy in pregnant or non-pregnant Hanwoo using proteomic analysis technique. Plasma samples (0, 2, 3, 4, 7, and 11 weeks after AI) were obtained from pregnant (P, n=3) or non-pregnant (NP, n=4) Hanwoo, respectively. To evaluate proteins differentially expressed, 2-dimensional electrophoresis (2DE) was conducted. Normalized protein spots were selected for the significant expression variation deviated over two fold in its expression level between two groups. Molecular functions of the proteins were DNA binding, protein binding, hemoglobin binding, ferrochelatase and transporter activity and arylestera, respectively. According to western blotting, haptoglobin was specifically expressed only in NP group during early pregnancy; however, paraoxonase 1 was highly expressed in pregnant group. Based on these results, pregnancy was maintained successfully by the activation of specific plasma proteins associated with immune system and antioxidant regulation during early pregnancy in Hanwoo

ABSTRACT   서론   재료 및 방법    공시동물    호르몬 분석    혈장 단백질 추출    2차원 전기영통    질량분석을 위한 단백질 절편화    단백질 통정    Western Blot Analysis    통계분석   결과   고찰   인용문헌

• 김평희(농촌진흥청 국립축산과학원 동물바이오광학과) | Pyung-Hee Kim
• 권대진(농촌진흥청 국립축산과학원 동물바이오광학과) | Dae Jin Kwon
• 오건봉(농촌진흥청 국립축산과학원 동물바이오광학과) | Keon-Bon Oh
• 이휘철(농촌진흥청 국립축산과학원 동물바이오광학과) | Hwi-Cheul Lee
• 양병철(농촌진흥청 국립축산과학원 동물바이오광학과) | Byoung-Chul Yang
• 임기순(농촌진흥청 국립축산과학원 동물바이오광학과) | Gi-Sun Im
• 민관식(농촌진흥청 국립축산과학원 동물바이오광학과) | Kwan-Sik Min
• 윤종택(한경대학교 생물환경보통전문대학원) | Jong-Taek Yoon
• 진동일(한경대학교 생물환경보통전문대학원) | Dong-Il Jin
• 박수봉(충남대학교 축산학과) | Soo-Bong Park
• 황성수(농촌진흥청 국립축산과학원 동물바이오광학과) | Seongsoo Hwang Corresponding author