Acyl-CoA synthetase 4(ACSL4) is an arachidonate-preferring enzyme abundant in steroidogenic tissues and postulated to modulate eicosanoid production. The human and mouse ACSL4 gene are mapped on chromosome X. The female mice heterozygous for ACSL4 deficiency became pregnant less frequently and produced small litters, with 40% of embryos surviving gestation. In this study, we examined the regulation of ACS4 by estradiol-17β and progesterone (P4) in the human endometrial cancer cell line HTB-1B. ACSL4 mRNA was increased in a dose-dependent manner. Also, expression of ACSL4 gene was up-regulated in a time-dependent manner in HTB-1B cells. However, combined treatment with progesterone and estradiol-17β modestly decreased the levels of ACS4L mRNA as compared with the estradiol-17β and progesterone respectively. Overall, these results suggest that the ACSL4 gene is regulated by progesterone and estradiol-17β in the HTB-1B cells.

ABSTRACT   서론   재료 및 방법    세포배양    Total RNA의 제조 및 RT-PCR    Real-time PCR    결과    Progesterone과 Estradiol-17β의 농도에 따른 ACSL4 유전자의 발현 조절    Progesterone과 Estradiol-17β의 처리시간 따른 ACSL4 유전자의 발현 조절    Progesterone과 Estradiol-17β의 연속 처리에 따른 ACSL4 유전자의 발현 조절   고찰   인용문헌

• 박효영(전남대학교 농업생명과학대학 동물자원학부) | Hyo Young Park
• 강만종(전남대학교 농업생명과학대학 동물자원학부) | Man-Jong Kang Corresponding author